The Healing Effect of Resveratrol on Ischemic Tissue Injury in an Experimental Carbon Monoxide Poisoning Model

Year 2020, , 288 - 296, 01.09.2020

Aynur Sahin Sinan Pasli Mustafa Cicek Suleyman Caner Karahan Ahmet Mentese Esin Yulug Yunus Karaca Ozgur Tatli

https://doi.org/10.30621/jbachs.2020.1197

Cited By: 1

Abstract

Purpose: To investigate the healing effect of resveratrol on ischemia, inflammation and oxidative injury in brain and heart tissue of rats in an experimentally induced carbonmonoxide CO intoxication model. Methods: Twenty-eight mature female Spraque Dawley rats were randomized into four groups of seven animals each. Groups I and II inhaled room air for 60 minutes. Groups III and IV inhaled a high concentration of a 5000 ppm CO gas mixture at 4 L/min until loss of consciousness. Blood CO levels were measured from 1 mL blood specimens collected from the tail vein. Groups I and III received 1 mL of normal saline while groups II and IV received 25 mg/kg resveratrol intraperitoneally. At the end of 48 hour, the rats were sacrified and later blood, brain and heart tissue samples were taken for histopathological and biochemical evaluation. Neuron damage scores, percentages of degenerate neurons and apoptosis rates in the cortex and hypothalamus were calculated in brain tissues. Myocyte degeneration, vascular congestion and myocyte apoptosis rates were calculated in heart tissues. Total antioxidant TAS , total oxidant TOS and oxidative stress index OSI levels were also calculated from the tissue samples. Results: Histopathological evaluation revealed that resveratrol significantly reduced tissue damage in the brain when groups III and IV were compared p

Keywords

CO poisoning, resveratrol, brain, heart

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