Optimization of Different Surface Modifications for Binding of Tumor Cells in a Microfluidic Systems

Abstract

Objectives:Microfluidic technology is a fast-growing area and provide high-efficient MEMS Micro-Electro-Mechanical-Systems sensor integration platform that helps to advance healthcare systems. Due to proper the chemical and mechanical properties of polymers, PDMS Polydimethylsiloxane 6 and PMMA Poly-methyl-methacrylate , they became on the best candidate for health care studies in microfluidic studies 7 . Besides, they perform great optical properties for observation of living cell experiments. To increase their performance, surface interactions works with cells, modification techniques are widely used in microfluidic chips. In this paper, our primary purpose is to modify such polymers and glass with matrigel, PDA and APTES so as to increase cell-surface interaction.Patients and Methods:Cells were seeded into the micro-channels that is modified with 1%, 3% and 5% matrigel. The cell culture were observed 48-hours and images were taken. In the next stage, static culture experiments were performed on glass surfaces modified with PDA polydopamine and APTES 3-Aminopropyl triethoxysilane . 3-hour images were received.Results:The area filled by the cells was calculated using the ImageJ software ver.1.149. In the Matrigel modified chips, at the end of the 48th hour, the surface area of the cells reached to 22.58%-29.14- 26.97% for the matrigel sample rates 1%, 3% and 5%, respectively. In PDA, APTES modified surfaces dissolved in ethanol and APTES modified surface dissolved in water , the cells overlaid on surface after 3rd hour 5,32% 8,08%; and 3,33%, respectively.Conclusion:The tendency of the cells to attach and colonize on increasing matrigel concentrations and on PDA, APTES modified surfaces was higher than the unmodified surface

Keywords

• Microfluidic Chips,Surface Modification,Cell Culture

References

1. Zhang X, Jones P, SHaswell S. Attachment and detachment of living cells on modified microchannel surfaces in a microfluidic-based lab- on-a-chip system. Chem Eng J 2008;135:S82-S88. [CrossRef]
2. Sakamoto C, Yamaguchi N. Yamada M, Nagase H, Seki M, Nasu M. Rapid quantification of bacterial cells in potable water using a simplified microfluidic device. J Microbiol Methods 2007;68:643– 647. [CrossRef]
3. Timur S. Protein Analitiği. Bölüm: Protein Chip’ler. Telefoncu A, Kılınç A, editörler. İzmir, Bornova: Ege Üniversitesi Basımevi; 2010.
4. Manz A, Becker H, editors. Microsystem Technology in Chemistry and Life Sciences. Berlin: Springer Verlag; 1998.
5. Zhang Z, Nagrath S. Microfluidics and cancer: are we there yet? Biomed Microdevices 201315:595–609. [CrossRef]
6. Fujii T. PDMS-based microfluidic devices for biomedical applications. Microelectron Eng 2002;61-62:907–914. [CrossRef]
7. Mas Haris MRH, Kathiresan S, Mohan S. FT-IR and FT-Raman spectra and normal coordinate analysis of polymethylmethacrylate. Der Pharma Chem 2010;2:316–323.
8. Prakash S, Long TM, Selby JC, Moore JS, Shannon MA. “Click” Modification of Silica Surfaces and Glass Microfluidic Channels. Anal Chem 2007;79:1661–1667. [CrossRef]

9. Park S, Joo YK, Chen Y. Dynamic adhesion characterization of cancer cells under blood flowmimetic conditions: effects of cell shape and orientation on drag force. Microfluidics Nanofluidics 2018;22:108. [CrossRef]
10. Kleinman HK, Martin GR. Matrigel: basement membrane matrix with biological activity. Semin Cancer Biol 2005;15:378–386. [CrossRef]
11. Lee H, Dellatore SM, Miller WM, Messersmith PB. Mussel-inspired surface chemistry for multifunctional coatings. Science 2007;318:426– 430. [CrossRef]
12. Ding YH, Floren M, Tan W. Mussel-inspired polydopamine for bio- surface functionalization. Biosurf Biotribol 2016;2:121–136. [CrossRef]
13. Vansant EF, van Der Voort P, Vrancken KC. Characterization and Chemical Modication of the Silica Surface, Chapter 9. New York: Elsevier; 1995.
14. Blitz JP, Shreedhara Murthy RS, Leyden DE. The role of amine structure on catalytic activity for silylation reactions with Cab-O-Sil. J Colloid Interface Sci 1988;126:387–392. [CrossRef]
15. Chaudhuri PK, Warkiani ME, Jing T, Kenry K, Lim CT. Microfluidics for research and applications in oncology. Analyst 2016;141:504–524. [CrossRef]
16. Chen J, Li J, Sun Y. Microfluidic approaches for cancer cell detection, characterization, and separation. Lab Chip 2012;12:1753–1767. [CrossRef]
17. Siyan W, Feng Y, Lichuan Z, et al. Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance. J Pharm Biomed Anal 2009;49:806–810. [CrossRef]
18. Zhang C, Gong L, Xiang L, et al. Deposition and Adhesion of Polydopamine on the Surfaces of Varying Wettability. ACS Appl Mater Interfaces 2017;9:30943–30950. [CrossRef]