Adverse Effects to Suckling Mice Following Indirect Exposure to a Pesticide Mixture and Ameliorative Effect of α-Tocopherol Coadministration

Year 2017, , 71 - 78, 01.09.2017

Sameeh Mansour Mohamed Abdel-mageed Khaled Mohamed Marwa Gad Laurence Gamet-payrastre

https://doi.org/10.5152/jbachs.2017.227

Cited By: 1

Abstract

Purpose: The present study was conducted to evaluate the oxidative damage and biochemical and histopathological alterations in suckling mice whose mothers were exposed to a mixture of three pesticides atrazine “A,” chlorpyrifos “C,” and endosulfan “E”; ACE with and without vitamin E α-tocopherol supplementation.Methods: During gestation, the mouse dams were fed a diet free of chemicals. Just after parturition and up to offspring weaning ca. 20 days , the mouse dams received one of the following diets: a a diet free of ACE, b a diet enriched with ACE at Acceptable Daily Intake ADI : 0.005, 0.01, and 0.006 mg/kg bw/day , c a diet free of ACE+oral vitamin E 100 ul/mouse/day , or d a diet enriched with ACE+oral vitamin E. Drinking water was allowed ad libitum up to 42 days. After weaning, blood samples and organs were collected from pups and dams for analyses. Results: Compared with the control results of dams or their pups, the ACE mixture induced high elevation in AST, ALT, ALP, urea, and MDA and high decline in BuChE, SOD, and CAT. The suckling pups were more affected than the dams with respect to alterations in MDA and BuChE activities, whereas the opposite was observed with respect to SOD activities. Vitamin E supplementation alleviated the oxidative stress exerted by the tested mixture. Conclusion: The findings of this study explore extension of the adverse effects of the ACE mixture to mouse offspring and the powerful ameliorating effect of vitamin E supplementation

Keywords

Pesticide mixture, lactation exposure, oxidative stress, vitamin E, amelioration

References

• Anderson HA, Wolff MS. Environmental contaminations in human milk. J Expo Anal Environ Epidemiol 2000; 10:755-760. [CrossRef]
• Nawaz A, Razpotnik A, Rouimi P, de Sousa G, Cravedi JP, Rahma- ni R. Cellular impact of combinations of endosulfan, atrazine, and chlorpyrifos on human primary hepatocytes and HepaRG cells after short and chronic exposures. Cell Biol Toxicol 2014; 30:17-29. [CrossRef]
• Rouimi P, Zucchini-Pascal N, Dupont G, et al. Impacts of low doses of pesticide mixtures on liver cell defence systems. Toxicol Vitro 2012; 26:718-726. [CrossRef]
• ATSDR. Toxicological Profile for Endosulfan. Agency for Toxic Sub- stances and Disease Registry, Division of Toxicology and Human Health Sciences/Environmental Toxicology Branch, 1600 Clifton Road, NE Mailstop F-57 Atlanta, Georgia 30329-4027; 2015.
• Mansour SA, Gamet-Payrastre L, Mohamed DA, Gad MF. Ame- liorative effect of vitamin E to mice dams and their pups following exposure of mothers to atrazine during gestation and lactation pe- riods. ScienceJet 2013; 32:3.
• Mansour SA, Mohamed DA, Gamet-Payrastre L. Indirect exposure of mice pups to endosulfan via their dams during gestation and lac- tation periods and the ameliorative effect of vitamin E. Hum Exper Toxicol 2014; 33:911-927. [CrossRef]
• Mansour SA, Gamet-Payrastre L. Ameliorative effect of vitamin E to mouse dams and their pups following exposure of mothers to chlorpyrifos during gestation and lactation periods. Toxicol Industr Health 2016; 32: 1179-1196. [CrossRef]
• Carpenter DO, Arcaro K, Spink DC. Understanding the human health effects of chemical mixtures. Environ Health Perspec 2002; 110: 25-42. [CrossRef]
• Belden JB, Lydy MJ. Impact of atrazine on organophosphate insec- ticide toxicity. Environ Toxicol Chem 2000; 19:2266-2274. [CrossRef]
• DeLorenzo ME, Lorimar S. Individual and mixture toxicity of three pesticides; atrazine, chlorpyrifos, and chlorothalonil to the marine phytoplankton species Dunaliella tertiolecta. J Environ Sci Health B 2003; 5:529-538. [CrossRef]
• Abdollahi M, Mostafalou S, Pournourmohammadi S, Shadnia S. Ox- idative stress and cholinesterase inhibition in saliva and plasma of rats following sub chronic exposure to malathion. Comp Biochem Physiol C: Toxicol Pharmacol 2004; 137:29-34.
• Gawish A, Elhalwagy MEA. Which can attenuate hepatotoxicity induced by pesticides mixture natural or synthetic phenolic antioxi- dant. Nat Sci 2009; 5:29-44.
• Verhagen H, Buijsse B, Jansen E, Bueno-de-Mesquita B. The state of antioxidant affairs. Nutr Today 2006; 41:244-250. [CrossRef]
• Merhi M, Demur C, Racaud-Sultan C, et al. Gender-linked haematopoi- etic and metabolic disturbances induced by a pesticide mixture ad- ministered at low dose to mice. Toxicology 2010; 267:80-90. [CrossRef]
• FAO/WHO. Pesticide Residues in Food 2010. Report of the Joint Meeting of the FAO Panel of Experts on Pesticide Residues in Food and the Environment and the WHO Core Assessment Group on Pesticide Residues, Rome, Italy; September 2010.
• Kalender S, Kalender Y, Ogutcu A, Uzunhisarcikli M, Durak D, Açikgoz F. Endosulfan-induced cardiotoxicity and free radical metabolism in rats: the protective effect of vitamin E. Toxicology 2004; 202:227-235. [CrossRef]
• Fishbeck KL, Rasmussen KM. Effect of repeated cycles on maternal nutritional status, lactational performance and litter growth in ad libitum fed and chronically food. J Nutr 1987; 117:1967-1975.
• Brunt EM, Janney CG, Di Bisceglie AM, Neuschwander-Tetri BA, Ba- con BR. Nonalcoholic steatohepatitis: a proposal for grading and staging the histological lesions. Am J Gastroenterol 1999; 94:2467- 2474. [CrossRef]
• Infante-Rivard C, Labuda D, Krajinovic M, Sinnett D. Risk of child- hood leukemia associated with exposure to pesticides and with gene polymorphisms [comment]. Epidemiology 1999; 10:481-487. [CrossRef]
• Perera FP, Illman SM, Kinney PL, et al. The challenge of prevent- ing environmentally related disease in young children: communi- ty-based research in New York City. Environ Health Perspec 2002; 110:197-204. [CrossRef]
• Scheuplein R, Charnley G, Dourson M. Differential sensitivity of chil- dren and adults to chemical toxicity I. Biological basis. Regul Toxicol Pharmacol 2002; 35:429-447. [CrossRef]
• Kostka G, Kopeć-Szlezak J, Palut D. Early hepatic changes induced in rats by two hepatocarcinogenicorganohalogen pesticides: bro- mopropylate and DDT. Carcinogenesis 1996; 17:407-412. [CrossRef]
• Rajesh MG, Latha MS. Preliminary evaluation of the antihepatotox- ic activity of Kamilari, a polyherbal formulation. J Ethnopharmacol 2004; 91:99-104. [CrossRef]
• Xing-Jiu H, Yang-Kyu C, Hyung-Soon I, Oktay Y, Euisik Y, Hak-Sung K. Aspartate Aminotransferase (AST/GOT) and Alanine Amino- transferase (ALT/GPT) detection techniques. Sensors 2006; 6:756- 782. [CrossRef]
• Walmsley RN, White GH. A guide to diagnostic clinical chemistry. 3rd ed. London, UK: Blackwell Publication; 1994, 543.
• Brown SS, Kalow W, Pilz W, Whittaker M, Woronick CL. The plasma cholinesterases: a new perspective. Adv Clin Chem 1981; 22:1-123. [CrossRef]
• Moss DM, Henderson AR. Tietz Textbook of Clinical Enzymology. In: Burtis CA, Ashwood ER, editors. WB Saunders Co: Philadelphia, PA, USA; 1999; 617-721.
• Ranjbar A, Pasalar P, Abdollahi M. Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorous pesticide manufacturing workers. Hum Exper Toxicol 2002; 21:179-182. [CrossRef]
• Mansour SA, Mossa AH. Adverse effects of lactational exposure to chlorpyrifos in suckling rats. Hum Exp Toxicol 2010; 29 :77-92. [CrossRef]
• Mansour SA, Mossa AH. Adverse effects of exposure to low doses of chlorpyrifos in lactating rats. Toxicol Industr Health 2011; 27:213-224. [CrossRef]
• McCord JM, Fridovich I. Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244:6049-6055.
• Lahouel A, Kebieche M, Lakroun Z, et al. Chronic exposure of female rats to a low dose POPs mixture induced oxidative stress in brain cytosol and mitochondria. Int Res J Earth Sci 2015; 3: 1-8.
• Horwitt MK. Vitamin E: a reexamination. Am J Clin Nutr 1976; 29:569-578.
• de Castro VLS, Chiorato SH. Effects of separate and combined expo- sure to the pesticides methamidophos and chlorothalonil on the de- velopment of suckling rats. Int J Hyg Environ Health 2007; 210:169-176. [CrossRef]