BibTex RIS Cite

The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion

Year 2018, Volume: 2 Issue: 3, 68 - 75, 01.09.2018
https://doi.org/10.30621/jbachs.2018.432

Abstract

Objectives: The effects of ozone treatment on direct mechanical injury and the inflammation secondary to the injury resulting from blunt thoracic trauma were evaluated with the histopathological results obtained in our study.Patients and Methods: 24 adult male Wistar albino rats of the same colony with an average weight of 200-250 gr were used in our study.The rats were divided into 3 groups. First group were anaesthetized with ketamine HCl/xylazine. Second group were given contusion with isolated unilateral lung contusion. Third group were given 30 µgr/ml ozone at a dose of 1.1mg/kg intrarectally after the contusion for five days.Four different parameters; atelectasis, parenchymal inflammation, perivascular mononuclear inflammation and bronchial injury, were used in histopathological evaluation and all parameters were scaled.Results: Cell concentration, perivascular mononuclear inflammation and leukocyte infiltration were significantly higher in the lung tissues of all the rats in the trauma group compared to the anesthesia group. However, when the control, trauma and ozone groups were compared, there was no significant difference in terms of alveolar edema and bronchial injury. Similarly, among subgroubs of trauma and ozone groups, there was not a statistical significance in terms of histopathological data.Conclusion: When inflammatory stages and cell distribution were compared between the study groups, advanced acute inflammatory changes were found on the fifth day of inflammation in both contusion groups and no significant difference was observed in terms of inflammatory response pattern. Consequently, no positive effect of ozone treatment was shown on the acute and subacute inflammatory response in decreasing the primary and secondary injury caused by the trauma

References

  • Eren MN, Balcı AE. Toraks Travmaları. In: Ökten İ, Güngör A, editors. Göğüs Cerrahisi. Ankara: Sim Matbaacılık; 2003. p.661–688.
  • Battistella FD, Benfield JR, Blunt and Penetrating Injuries of the Chest Wall, Pleura, Lungs. In: Shields TW, editor. Genaral Thoracic Surgery, 5th ed. Philadelphia 2000; p.815–836.
  • Raghavendran K, Davidson BA, Woytash JA, et al. The evolution of isolated bilateral lung contusion from blunt chest trauma in rats: cellular and cytokine responses. Shock 2005;24:132–138. [CrossRef]
  • Raghavendran K, Davidson BA, Helinski JD, et al. A rat model for isolated bilateral lung contusion from blunt chest trauma. Anesth Analg 2005;101:1482–1489. [CrossRef]
  • Oppenheimer L, Craven KD, Forkert L, Wood LD. Pathophysioogy of pulmonary contusion in dogs. J Appl Physiol 1979;47:718–728. [CrossRef]
  • Fulton RL, Peter ET, Wilson JN. Pathophysiology and treatment of pulmonary contusions. J Trauma 1970;10:719–730.
  • Strohmaier W, Trupka A, Pfeiler C, et al. Bilateral lavage with diluted surfactan improves lung function after unilateral lung contusion in pigs. Crit Care Med 2005;33:2286–2293. [CrossRef]
  • Pepe PE, Potkin RT, Reus DH, Hudson CD, Carrico CJ. Clinical predictors of the adult respiratory distress syndrome. Am J Surg 1982;144:124–130. [CrossRef]
  • Bocci V. Physical-chemical properties of ozone. Natural production of ozone. The toxicology of ozone. In: OZONE A New Medical Drug, Chapter 1. Dordrecht: Springer 2005. p.5–8.
  • Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006;37:425–35. [CrossRef]
  • Re L, Mawsouf MN, Menendez S, Leon OS, Sanchez GM, Hernandez F. Ozone therapy: clinical and basic evidence of its therapeutic potential. Arch Med Res 2008;39:17–26. [CrossRef]
  • Van Gijn ME, Blankesteijn WM, Smits JF, Hierck B, Gittenberger-de Groot AC. Frizzled 2 is transiently expressed in neural crest containing areas during development of the heart and great arteries in the mouse. Anat Embryol 2001;203:185–192. [CrossRef]
  • Ward PA. Acute lung injury: how lung inflammatory response works. Eur Respir J 2003;22:22s–23s. [CrossRef]
  • Wang ND, Stevens MH, Doty DB, Hammond EH. Blunt chest trauma: an experimental model for heart and lung contusion. J Trauma 2003;54:744–749. [CrossRef]
  • Knöferl MW, Liener UC, Seitz DH, Perl M, Brückner UB, Kinzl L, Gebhard F. Cardiopulmonary, Histological, and Inflamatory Alterations After Lung Contusion in A Novel Mouse Model of Blunt Chest Trauma. Shock 2003;19:519–525. [CrossRef]
  • Proctor KG. Blood substitutes and experimental models of trauma. J Trauma 2003;54:S106–S109.
  • Lee CC, Marill KA, Carter WA, Crupi RS. A current concept of trauma- induced multiorgan failure. Ann Emerg Med 2001;38:170–176. [CrossRef]
  • Marshal JC. Inflamation, coagulupathy and pathogenesis of multiple organ disfunction syndrome. Crit Care Med 2001;29:S99–S106. [CrossRef]
  • Windsor AC, Mullen PG, Fowler AA. Acute lung injury; what have we learned from animal models? Am J Med Sci 1993;306:111–116. [CrossRef]
  • Bocci V, Paulesu L. Studies on the biological effects of ozone 1. Induction of interferon gamma on human leucocytes. Haematologica 1990;75:510–515.
  • Biedunkiewicz B, Tylicki L, Rachon D, et al. Natural killer cell activity unaffected by ozonated autohemotherapy in patients with end-stage renal disease on maintenance renal replacement therapy. Int J Artif Organs 2004;27:766–771. [CrossRef]
  • Bernardino Clavo, Gutiérrez D, Martín D, Suárez G, Hernández MA, Robaina F. Intravesical Ozone Therapy for Progressive Radiation- Induced Hematuria: J Altern Complement Med 2005;11:539–541. [CrossRef]
  • González R, Borrego A, Zamora Z, et al. Reversion by ozone treatment of acute nephrotoxicity induced by cisplatin in rats. Mediators Inflamm 2004;13:307–312. [CrossRef]
Year 2018, Volume: 2 Issue: 3, 68 - 75, 01.09.2018
https://doi.org/10.30621/jbachs.2018.432

Abstract

References

  • Eren MN, Balcı AE. Toraks Travmaları. In: Ökten İ, Güngör A, editors. Göğüs Cerrahisi. Ankara: Sim Matbaacılık; 2003. p.661–688.
  • Battistella FD, Benfield JR, Blunt and Penetrating Injuries of the Chest Wall, Pleura, Lungs. In: Shields TW, editor. Genaral Thoracic Surgery, 5th ed. Philadelphia 2000; p.815–836.
  • Raghavendran K, Davidson BA, Woytash JA, et al. The evolution of isolated bilateral lung contusion from blunt chest trauma in rats: cellular and cytokine responses. Shock 2005;24:132–138. [CrossRef]
  • Raghavendran K, Davidson BA, Helinski JD, et al. A rat model for isolated bilateral lung contusion from blunt chest trauma. Anesth Analg 2005;101:1482–1489. [CrossRef]
  • Oppenheimer L, Craven KD, Forkert L, Wood LD. Pathophysioogy of pulmonary contusion in dogs. J Appl Physiol 1979;47:718–728. [CrossRef]
  • Fulton RL, Peter ET, Wilson JN. Pathophysiology and treatment of pulmonary contusions. J Trauma 1970;10:719–730.
  • Strohmaier W, Trupka A, Pfeiler C, et al. Bilateral lavage with diluted surfactan improves lung function after unilateral lung contusion in pigs. Crit Care Med 2005;33:2286–2293. [CrossRef]
  • Pepe PE, Potkin RT, Reus DH, Hudson CD, Carrico CJ. Clinical predictors of the adult respiratory distress syndrome. Am J Surg 1982;144:124–130. [CrossRef]
  • Bocci V. Physical-chemical properties of ozone. Natural production of ozone. The toxicology of ozone. In: OZONE A New Medical Drug, Chapter 1. Dordrecht: Springer 2005. p.5–8.
  • Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res 2006;37:425–35. [CrossRef]
  • Re L, Mawsouf MN, Menendez S, Leon OS, Sanchez GM, Hernandez F. Ozone therapy: clinical and basic evidence of its therapeutic potential. Arch Med Res 2008;39:17–26. [CrossRef]
  • Van Gijn ME, Blankesteijn WM, Smits JF, Hierck B, Gittenberger-de Groot AC. Frizzled 2 is transiently expressed in neural crest containing areas during development of the heart and great arteries in the mouse. Anat Embryol 2001;203:185–192. [CrossRef]
  • Ward PA. Acute lung injury: how lung inflammatory response works. Eur Respir J 2003;22:22s–23s. [CrossRef]
  • Wang ND, Stevens MH, Doty DB, Hammond EH. Blunt chest trauma: an experimental model for heart and lung contusion. J Trauma 2003;54:744–749. [CrossRef]
  • Knöferl MW, Liener UC, Seitz DH, Perl M, Brückner UB, Kinzl L, Gebhard F. Cardiopulmonary, Histological, and Inflamatory Alterations After Lung Contusion in A Novel Mouse Model of Blunt Chest Trauma. Shock 2003;19:519–525. [CrossRef]
  • Proctor KG. Blood substitutes and experimental models of trauma. J Trauma 2003;54:S106–S109.
  • Lee CC, Marill KA, Carter WA, Crupi RS. A current concept of trauma- induced multiorgan failure. Ann Emerg Med 2001;38:170–176. [CrossRef]
  • Marshal JC. Inflamation, coagulupathy and pathogenesis of multiple organ disfunction syndrome. Crit Care Med 2001;29:S99–S106. [CrossRef]
  • Windsor AC, Mullen PG, Fowler AA. Acute lung injury; what have we learned from animal models? Am J Med Sci 1993;306:111–116. [CrossRef]
  • Bocci V, Paulesu L. Studies on the biological effects of ozone 1. Induction of interferon gamma on human leucocytes. Haematologica 1990;75:510–515.
  • Biedunkiewicz B, Tylicki L, Rachon D, et al. Natural killer cell activity unaffected by ozonated autohemotherapy in patients with end-stage renal disease on maintenance renal replacement therapy. Int J Artif Organs 2004;27:766–771. [CrossRef]
  • Bernardino Clavo, Gutiérrez D, Martín D, Suárez G, Hernández MA, Robaina F. Intravesical Ozone Therapy for Progressive Radiation- Induced Hematuria: J Altern Complement Med 2005;11:539–541. [CrossRef]
  • González R, Borrego A, Zamora Z, et al. Reversion by ozone treatment of acute nephrotoxicity induced by cisplatin in rats. Mediators Inflamm 2004;13:307–312. [CrossRef]
There are 23 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Fatma Ilknur Ulugun

Aydin Sanli This is me

Ensari Guneli This is me

Duygu Gurel This is me

Publication Date September 1, 2018
Published in Issue Year 2018 Volume: 2 Issue: 3

Cite

APA Ulugun, F. I., Sanli, A., Guneli, E., Gurel, D. (2018). The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion. Journal of Basic and Clinical Health Sciences, 2(3), 68-75. https://doi.org/10.30621/jbachs.2018.432
AMA Ulugun FI, Sanli A, Guneli E, Gurel D. The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion. JBACHS. September 2018;2(3):68-75. doi:10.30621/jbachs.2018.432
Chicago Ulugun, Fatma Ilknur, Aydin Sanli, Ensari Guneli, and Duygu Gurel. “The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion”. Journal of Basic and Clinical Health Sciences 2, no. 3 (September 2018): 68-75. https://doi.org/10.30621/jbachs.2018.432.
EndNote Ulugun FI, Sanli A, Guneli E, Gurel D (September 1, 2018) The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion. Journal of Basic and Clinical Health Sciences 2 3 68–75.
IEEE F. I. Ulugun, A. Sanli, E. Guneli, and D. Gurel, “The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion”, JBACHS, vol. 2, no. 3, pp. 68–75, 2018, doi: 10.30621/jbachs.2018.432.
ISNAD Ulugun, Fatma Ilknur et al. “The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion”. Journal of Basic and Clinical Health Sciences 2/3 (September 2018), 68-75. https://doi.org/10.30621/jbachs.2018.432.
JAMA Ulugun FI, Sanli A, Guneli E, Gurel D. The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion. JBACHS. 2018;2:68–75.
MLA Ulugun, Fatma Ilknur et al. “The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion”. Journal of Basic and Clinical Health Sciences, vol. 2, no. 3, 2018, pp. 68-75, doi:10.30621/jbachs.2018.432.
Vancouver Ulugun FI, Sanli A, Guneli E, Gurel D. The Effect of Ozone Treatment to the Inflammatory Response in Lung Contusion. JBACHS. 2018;2(3):68-75.