Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study

Year 2023, Volume: 7 Issue: 1, 507 - 515, 31.01.2023

Ahu Pakdemirli , Meral Sarper , Elif Güler Kazancı

https://doi.org/10.30621/jbachs.1210789

Abstract

Background and purpose: Mesenchymal stem cells are multipotent cells of mesodermal origin,
which have the ability to differentiate into other cells in the bone marrow microenvironment under
suitable conditions in vitro. Homeopathy is a holistic practice method that aims to improve health
status with personalized homeopathic medicines. At the same time, it is among the certified training
programs of the Traditional and Complementary Medicine Practices of the Ministry of Health. Studies
evaluating the effect of homeopathic remedies in the field of stem cells are rarely encountered in the
literature. As a matter of fact, the material and method parts of these studies are not clear enough. In
our study, the methodology of working with mesenchymal stem cells of homeopathic remedies has
been presented in detail and it has been aimed to observe their proliferative effects.

Material and Methods: Mesenchymal stem cells were exposed to 10%, 20%, 50% and 100% ratios
of homeopathic remedies (30°C potency: Aloe, Calendula, Hypericum, Staphysagria) for 24 and 48
hours. The percent viability of cells at 24 and 48 hours was evaluated with the MTT (3-4,5-dimethyl-
thiazolyl-2,5-diphenyltetrazolium bromide) test.

Results: Aloe vera C 30 – Staphysagria C30 – Calencula C30 – Hypericum C30 remedies were found
to have an effect on mesenchymal stem cell proliferation at all doses, and the results were statistically
more significant when maintained in 10% media.

Conclusion: It is thought that the methodology used in this study on working methods with
homeopathic remedies will contribute to the literature and many scientific studies to be done in the
future.

Keywords

mesenchymal stem cell, homeopathy, remedy, methodology, proliferation, traditional and complementary medicine

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