Research Article
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Year 2024, Volume: 8 Issue: 3, 726 - 733, 30.09.2024
https://doi.org/10.30621/jbachs.1540783

Abstract

Project Number

TSG-2022- 2576

References

  • Bhat SM, Badiger VA, Vasishta S, Chakraborty J, Prasad S, Ghosh S, Joshi MB. 3D tumor angiogenesis models: recent advances and challenges. J Cancer Res Clin Oncol 2021;147(12):3477-3494.
  • Baker BM, Chen CS. Deconstructing the third dimension: how 3D culture microenvironments alter cellular cues. J Cell Sci 2012;125(Pt 13):3015-3024.
  • Knight E, Przyborski S. Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro. J Anat 2015;227(6):746-756.
  • Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol 2011;21(12):745-754.
  • Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol 2007;8(10):839-845.
  • Yamada KM, Cukierman E. Modeling tissue morphogenesis and cancer in 3D. Cell 2007;130(4):601-610.
  • Birgersdotter A, Sandberg R, Ernberg I. Gene expression perturbation in vitro--a growing case for three-dimensional (3D) culture systems. Semin Cancer Biol 2005;15(5):405-412.
  • Rensen SS, Doevendans PA, van Eys GJ. Regulation and characteristics of vascular smooth muscle cell phenotypic diversity. Neth Heart J 2007;15(3):100-108.
  • Cukierman E, Pankov R, Stevens DR, Yamada KM. Taking cell-matrix adhesions to the third dimension. Science 2001;294(5547):1708-1712.
  • Edmondson R, Broglie JJ, Adcock AF, Yang L. Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors. Assay Drug Dev Technol 2014;12(4):207-218.
  • Hashimoto K, Yamashita K, Enoyoshi K, et al. The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation. J Biol Phys 2020;46(4):351-369.
  • O'Sullivan D, O'Neill L, Bourke P. Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture. ACS Omega 2020;5(39):25069-25076.
  • Chua P, Lim WK. The strategic uses of collagen in adherent cell cultures. Cell Biol Int 2023;47(2):367-373.
  • Karimi M, Mosaddad SA, Aghili SS, Dortaj H, Hashemi SS, Kiany F. Attachment and proliferation of human gingival fibroblasts seeded on barrier membranes using Wharton's jelly-derived stem cells conditioned medium: An in vitro study. J Biomed Mater Res B Appl Biomater 2024;112(1):e35368.
  • Ziaei Amiri F, Pashandi Z, Lotfibakhshaiesh N, Mirzaei-Parsa MJ, Ghanbari H, Faridi-Majidi R. Cell attachment effects of collagen nanoparticles on crosslinked electrospun nanofibers. Int J Artif Organs 2021;44(3):199-207.
  • Sorushanova A, Delgado LM, Wu Z, et al. The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development. Adv Mater 2019;31(1):e1801651.
  • Provenzano PP, Eliceiri KW, Inman DR, Keely PJ. Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration. Curr Protoc Cell Biol 2010;Chapter 10:Unit-10.17.
  • Rezvani Ghomi E, Nourbakhsh N, Akbari Kenari M, Zare M, Ramakrishna S. Collagen-based biomaterials for biomedical applications. J Biomed Mater Res B Appl Biomater 2021;109(12):1986-1999.
  • Owens GK, Kumar MS, Wamhoff BR. Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev 2004;84(3):767-801.
  • Kural MH, Billiar KL. Regulating tension in three-dimensional culture environments. Exp Cell Res. 2013;319(16):2447-2459.
  • Tracqui, P. Biophysical models of tumour growth. Reports on Progress in Physics 2009;72:056701.
  • Mack CP. Signaling mechanisms that regulate smooth muscle cell differentiation. Arterioscler Thromb Vasc Biol 2011;31(7):1495-1505.
  • Hynes RO. The extracellular matrix: not just pretty fibrils. Science 2009;326(5957):1216-1219. Frantz C, Stewart KM, Weaver VM. The extracellular matrix at a glance. J Cell Sci 2010;123(Pt 24):4195-4200.
  • Geiger B, Yamada KM. Molecular architecture and function of matrix adhesions. Cold Spring Harb Perspect Biol 2011;3(5):a005033.
  • Cummings CL, Gawlitta D, Nerem RM, Stegemann JP. Properties of engineered vascular constructs made from collagen, fibrin, and collagen-fibrin mixtures. Biomaterials 2004;25(17):3699-3706.

Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells

Year 2024, Volume: 8 Issue: 3, 726 - 733, 30.09.2024
https://doi.org/10.30621/jbachs.1540783

Abstract

Purpose: Collagen is a critical extracellular matrix (ECM) component that significantly influences cellular behaviors such as adhesion, migration, and proliferation. Optimizing collagen coating protocols is essential for developing accurate in vitro models, particularly for studying vascular smooth muscle cells (HVSMCs). The aim of this study was to optimize collagen coating protocols for in vitro models using HVSMCs by assessing cell morphology, adhesion potential, and viability under various collagen concentrations and incubation conditions.
Methods: HVSMCs were cultured on surfaces coated with different concentrations of Type 1 Rat Tail Collagen with different cell number (as 104 cells/well and 204 cells/well). The cells were incubated at various temperatures (4°C, 25°C, and 37°C). Morphological analysis was performed using phase-contrast microscopy to observe the alignment and phenotype of the cells. Cell adhesion was assessed using DAPI staining, and cell viability was evaluated using the Presto Blue assay after 96 hours of incubation.
Results: Collagen coating significantly influenced HVSMC behavior. The cells transitioned to a contractile phenotype, evidenced by tight, parallel bundle alignment, which is critical for maintaining vascular tone. Enhanced cell adhesion was observed in specific collagen-coated groups across different temperatures, particularly in the F, G, and H groups. Additionally, collagen coating did not significantly increase cell proliferation, making it suitable for in vitro vascular models. Optimal results were observed in groups seeded with 104 cells and incubated at 25°C and 37°C.
Conclusion: The study highlights the importance of optimizing extracellular matrix components like collagen in developing functional in vitro models. The identified optimal conditions for collagen coating will be valuable for future vascular modeling studies, providing a reliable foundation for in vitro research.

Ethical Statement

This study was conducted as part of the project "Effects of Different Dietary Components on Metastasis/Angiogenesis Pathway in Cancer," supported by the Dokuz Eylul University Scientific Research Projects. The project was approved by the Dokuz Eylul University Non-Invasive Research Ethics Committee (Date: 22.02.2023, No: 2023/05-32).

Supporting Institution

The study is funded by Dokuz Eylul University Scientific Research Projects Coordination Unit with project number TSG-2022- 2576. O.B. was supported by TUBITAK 2211C Domestic Priority Doctoral Scholarship Program, and Council of Higher Education 100/2000 scholarship in priority field program.

Project Number

TSG-2022- 2576

References

  • Bhat SM, Badiger VA, Vasishta S, Chakraborty J, Prasad S, Ghosh S, Joshi MB. 3D tumor angiogenesis models: recent advances and challenges. J Cancer Res Clin Oncol 2021;147(12):3477-3494.
  • Baker BM, Chen CS. Deconstructing the third dimension: how 3D culture microenvironments alter cellular cues. J Cell Sci 2012;125(Pt 13):3015-3024.
  • Knight E, Przyborski S. Advances in 3D cell culture technologies enabling tissue-like structures to be created in vitro. J Anat 2015;227(6):746-756.
  • Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol 2011;21(12):745-754.
  • Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol 2007;8(10):839-845.
  • Yamada KM, Cukierman E. Modeling tissue morphogenesis and cancer in 3D. Cell 2007;130(4):601-610.
  • Birgersdotter A, Sandberg R, Ernberg I. Gene expression perturbation in vitro--a growing case for three-dimensional (3D) culture systems. Semin Cancer Biol 2005;15(5):405-412.
  • Rensen SS, Doevendans PA, van Eys GJ. Regulation and characteristics of vascular smooth muscle cell phenotypic diversity. Neth Heart J 2007;15(3):100-108.
  • Cukierman E, Pankov R, Stevens DR, Yamada KM. Taking cell-matrix adhesions to the third dimension. Science 2001;294(5547):1708-1712.
  • Edmondson R, Broglie JJ, Adcock AF, Yang L. Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors. Assay Drug Dev Technol 2014;12(4):207-218.
  • Hashimoto K, Yamashita K, Enoyoshi K, et al. The effects of coating culture dishes with collagen on fibroblast cell shape and swirling pattern formation. J Biol Phys 2020;46(4):351-369.
  • O'Sullivan D, O'Neill L, Bourke P. Direct Plasma Deposition of Collagen on 96-Well Polystyrene Plates for Cell Culture. ACS Omega 2020;5(39):25069-25076.
  • Chua P, Lim WK. The strategic uses of collagen in adherent cell cultures. Cell Biol Int 2023;47(2):367-373.
  • Karimi M, Mosaddad SA, Aghili SS, Dortaj H, Hashemi SS, Kiany F. Attachment and proliferation of human gingival fibroblasts seeded on barrier membranes using Wharton's jelly-derived stem cells conditioned medium: An in vitro study. J Biomed Mater Res B Appl Biomater 2024;112(1):e35368.
  • Ziaei Amiri F, Pashandi Z, Lotfibakhshaiesh N, Mirzaei-Parsa MJ, Ghanbari H, Faridi-Majidi R. Cell attachment effects of collagen nanoparticles on crosslinked electrospun nanofibers. Int J Artif Organs 2021;44(3):199-207.
  • Sorushanova A, Delgado LM, Wu Z, et al. The Collagen Suprafamily: From Biosynthesis to Advanced Biomaterial Development. Adv Mater 2019;31(1):e1801651.
  • Provenzano PP, Eliceiri KW, Inman DR, Keely PJ. Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration. Curr Protoc Cell Biol 2010;Chapter 10:Unit-10.17.
  • Rezvani Ghomi E, Nourbakhsh N, Akbari Kenari M, Zare M, Ramakrishna S. Collagen-based biomaterials for biomedical applications. J Biomed Mater Res B Appl Biomater 2021;109(12):1986-1999.
  • Owens GK, Kumar MS, Wamhoff BR. Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev 2004;84(3):767-801.
  • Kural MH, Billiar KL. Regulating tension in three-dimensional culture environments. Exp Cell Res. 2013;319(16):2447-2459.
  • Tracqui, P. Biophysical models of tumour growth. Reports on Progress in Physics 2009;72:056701.
  • Mack CP. Signaling mechanisms that regulate smooth muscle cell differentiation. Arterioscler Thromb Vasc Biol 2011;31(7):1495-1505.
  • Hynes RO. The extracellular matrix: not just pretty fibrils. Science 2009;326(5957):1216-1219. Frantz C, Stewart KM, Weaver VM. The extracellular matrix at a glance. J Cell Sci 2010;123(Pt 24):4195-4200.
  • Geiger B, Yamada KM. Molecular architecture and function of matrix adhesions. Cold Spring Harb Perspect Biol 2011;3(5):a005033.
  • Cummings CL, Gawlitta D, Nerem RM, Stegemann JP. Properties of engineered vascular constructs made from collagen, fibrin, and collagen-fibrin mixtures. Biomaterials 2004;25(17):3699-3706.
There are 25 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Research Article
Authors

Özge Bayrak 0000-0002-6223-1272

Gizem Çalıbaşı Koçal 0000-0002-3201-4752

Yasemin Başbınar 0000-0001-9439-2217

Meltem Alper 0000-0001-6359-9979

Serdar Bayrak 0000-0003-1458-9023

Project Number TSG-2022- 2576
Publication Date September 30, 2024
Submission Date August 29, 2024
Acceptance Date September 7, 2024
Published in Issue Year 2024 Volume: 8 Issue: 3

Cite

APA Bayrak, Ö., Çalıbaşı Koçal, G., Başbınar, Y., Alper, M., et al. (2024). Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells. Journal of Basic and Clinical Health Sciences, 8(3), 726-733. https://doi.org/10.30621/jbachs.1540783
AMA Bayrak Ö, Çalıbaşı Koçal G, Başbınar Y, Alper M, Bayrak S. Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells. JBACHS. September 2024;8(3):726-733. doi:10.30621/jbachs.1540783
Chicago Bayrak, Özge, Gizem Çalıbaşı Koçal, Yasemin Başbınar, Meltem Alper, and Serdar Bayrak. “Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells”. Journal of Basic and Clinical Health Sciences 8, no. 3 (September 2024): 726-33. https://doi.org/10.30621/jbachs.1540783.
EndNote Bayrak Ö, Çalıbaşı Koçal G, Başbınar Y, Alper M, Bayrak S (September 1, 2024) Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells. Journal of Basic and Clinical Health Sciences 8 3 726–733.
IEEE Ö. Bayrak, G. Çalıbaşı Koçal, Y. Başbınar, M. Alper, and S. Bayrak, “Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells”, JBACHS, vol. 8, no. 3, pp. 726–733, 2024, doi: 10.30621/jbachs.1540783.
ISNAD Bayrak, Özge et al. “Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells”. Journal of Basic and Clinical Health Sciences 8/3 (September 2024), 726-733. https://doi.org/10.30621/jbachs.1540783.
JAMA Bayrak Ö, Çalıbaşı Koçal G, Başbınar Y, Alper M, Bayrak S. Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells. JBACHS. 2024;8:726–733.
MLA Bayrak, Özge et al. “Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells”. Journal of Basic and Clinical Health Sciences, vol. 8, no. 3, 2024, pp. 726-33, doi:10.30621/jbachs.1540783.
Vancouver Bayrak Ö, Çalıbaşı Koçal G, Başbınar Y, Alper M, Bayrak S. Effect of Collagen-Coating Variations on the Morphology and Viability of Human Vascular Smooth Muscle Cells. JBACHS. 2024;8(3):726-33.