Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

Year 2025, Volume: 9 Issue: 3, 457 - 464, 30.09.2025

Seher Elif Koçoğlu , Leyla Çimen , Nur Tufanoğlu , Semih Yılmaz , Aysun Çetin

https://doi.org/10.30621/jbachs.1588609

Abstract

Project Number

TYL-2018-8508

Role of CAPE on Protein Profile and Oxidative Stress Panel in Healthy Liver Tissue

Abstract

Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE), one of the active ingredients of propolis, on protein expression differences and some antioxidant parameters in rat liver.
Methods: 3 groups were formed as control (ip SF), CAPE 10 (ip 10 μmol/kg CAPE) and CAPE 50 (ip 10 μmol/kg CAPE for 5 days). Protein expression differences were analyzed by SDS-PAGE gel electrophoresis, GSH-Px, SOD enzyme activities and MDA levels were measured by ELISA.
Results: The amount of protein with a molecular weight (MW) of 53 kDa in CAPE 10 was found to be higher than control group (p<0.05). The amount of protein with a MW of 35 kDa in CAPE 10 group was found to be higher than control (p<0.001). The amount of protein with a MW of 14 kDa in CAPE 50 group was found to be higher than control (p<0.05). MDA levels were statistically higher in CAPE groups compared to control group (p<0.001). GSH-Px and SOD activities in CAPE groups was found to be higher than control group, and the difference was not statistically significant.
Conclusions: CAPE didn’t show the expected antioxidant effect on liver tissues, a prooxidant effect was observed.

Keywords

propolis , caffeic acid phenethyl ester , liver , prooxidant , antioxidant , oxidative stress

Project Number

TYL-2018-8508

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