Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum)

Sara Yasemin; Nezihe Köksal; Saadet Büyükalaca

Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum)

Abstract

In this study, we investigated the disinfection methods and germination conditions for seeds of sea daffodil (Pancratium maritimum). Seeds were exposed to 20% and 40% NaOCl (Sodium hypochlorite) solutions for 15, 20, and 25 minutes, as disinfection treatments. The rate of 20% NaOCl was the most appropriate concentration for percentage of seed germination (58%) and plant formation (34%). Effects of three culture media on seed germination, plant formation, and growth were investigated: A. Agar (4 gl-1) with sucrose (30 gl-1); B. MS (Murashige - Skoog) with agar (4 gl-1) and sucrose (30 gl-1); C. MS with agar (4 gl-1), sucrose (30 gl-1), and plant growth regulators (1 mgl-1 BA, 0.1 mgl-1 2,4-D). Evaluated plant growth parameters were total plant weight; number, width, and length of leaf; thickness and length of root; and diameter, length, and weight of bulb. All culture media were found to be successful in terms of in vitro germination of the seeds. The most suitable medium for plant formation and all the plant growth parameters were found to be Medium B. This is an innovative report in that it has been thoroughly investigated in terms of disinfection practices and seed germination media with different evaluation parameters.

Keywords

Amaryllidaceae, Disinfection, Growth parameters, Plant formation, Seed germination

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Details

Primary Language

English

Subjects

Agricultural Biotechnology (Other)

Journal Section

Research Article

Authors

Sara Yasemin ^*
Türkiye

Nezihe Köksal
Türkiye

Saadet Büyükalaca
Türkiye

Publication Date

May 25, 2018

Submission Date

March 8, 2018

Acceptance Date

April 13, 2018

Published in Issue

Year 2018 Volume: 12 Number: 34

IZ

https://izlik.org/JA95AG64PM

APA

Yasemin, S., Köksal, N., & Büyükalaca, S. (2018). Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum). Journal of Biological and Environmental Sciences, 12(34), 13-22. https://izlik.org/JA95AG64PM

AMA

1.Yasemin S, Köksal N, Büyükalaca S. Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum). JBES. 2018;12(34):13-22. https://izlik.org/JA95AG64PM

Chicago

Yasemin, Sara, Nezihe Köksal, and Saadet Büyükalaca. 2018. “Effects of Disinfection Conditions and Culture Media on in Vitro Germination of Sea Daffodil (Pancratium Maritimum)”. Journal of Biological and Environmental Sciences 12 (34): 13-22. https://izlik.org/JA95AG64PM.

EndNote

Yasemin S, Köksal N, Büyükalaca S (May 1, 2018) Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum). Journal of Biological and Environmental Sciences 12 34 13–22.

IEEE

[1]S. Yasemin, N. Köksal, and S. Büyükalaca, “Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum)”, JBES, vol. 12, no. 34, pp. 13–22, May 2018, [Online]. Available: https://izlik.org/JA95AG64PM

ISNAD

Yasemin, Sara - Köksal, Nezihe - Büyükalaca, Saadet. “Effects of Disinfection Conditions and Culture Media on in Vitro Germination of Sea Daffodil (Pancratium Maritimum)”. Journal of Biological and Environmental Sciences 12/34 (May 1, 2018): 13-22. https://izlik.org/JA95AG64PM.

JAMA

1.Yasemin S, Köksal N, Büyükalaca S. Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum). JBES. 2018;12:13–22.

MLA

Yasemin, Sara, et al. “Effects of Disinfection Conditions and Culture Media on in Vitro Germination of Sea Daffodil (Pancratium Maritimum)”. Journal of Biological and Environmental Sciences, vol. 12, no. 34, May 2018, pp. 13-22, https://izlik.org/JA95AG64PM.

Vancouver

1.Sara Yasemin, Nezihe Köksal, Saadet Büyükalaca. Effects of Disinfection Conditions and Culture Media on in vitro Germination of Sea Daffodil (Pancratium maritimum). JBES [Internet]. 2018 May 1;12(34):13-22. Available from: https://izlik.org/JA95AG64PM

Journal of Biological and Environmental Sciences is the official journal of Bursa Uludag University

Bursa Uludag University, Gorukle Campus, 16059, Bursa, Türkiye.