The Effects of Different Diet Total Volatile Nitrogen Levels on Blood Biochemical Parameters in Broilers

Year 2015, Volume: 9 Issue: 27, 151 - 158, 01.04.2016

Mohammad Ghasemi-sadabadi Yahya Ebrahimnezhad Behrad Eshratkhah Naser Maheri-sis

Abstract

A study was conducted to determine the effects of different diet total volatile nitrogen levels on blood biochemical parameters in broilers. A total of 320 one day old, Ross 308 broiler chicks, were randomly allocated to one of the 20 floor pens in a completely randomized design with five treatments and four replicate groups and sixteen chicks in each group. The aim of using different levels of urea in this study due to created various levels of total volatile nitrogen in the diet. Dietary treatments consisted five different levels of urea including zero (control), 0.5, 1, 1.5 and 2 percentages of urea in the diets. The total content of volatile nitrogen (TVN) measured after adding different levels of urea in diet and found as 13.30, 14.95, 17.26, 23.26 and 27.47 mg per 100 g respectively in starter diets and 16.66, 15.02, 17.81, 24.66 and 26.25 TVN mg per 100 g growing diets, respectively. The chicks were fed without adding urea during the first week. At 42 days of age, four chickens (two male and two female) from each pen were selected and blood samples collected from taken wing vein. Glucose, triglyceride (TG), cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL), phosphorus, calcium, albumin, total protein, globulin, uric acid and urea were measured. The results indicated that high level of total volatile nitrogen in diet significantly increased blood urea, uric acid, TG, cholesterol, LDL, phosphorus, total protein and globulin levels, while significantly decreased blood glucose level in our study (P<0.05). In conclusion, the results of this study concluded that, increasing TVN in diet had deleterious effect on blood biochemical parameters in broilers.

Keywords

Total volatile nitrogen , Urea , Blood biochemical parameter , Broiler

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