Clastogenic studies on Tandaha Dam water in Asser

Abstract

Abstract Clastogenic effects of home wastes and agricultural contaminates of Tandaha dam located at Alahad municipality in the southwestern part of Saudi Arabia were investigated in peripheral erythrocytes of Rana ridibunda. Examination of blood smears showed that the formation of micronuclei significantly increased during one year (f=9.89, df=3) with p value < 0.05 and were more abundant compared to the control group. This increase in the formation of micronucleus indicates that agricultural pollution increases the risk of clastogenic effects on peripheral erythrocytes of Rana ridibunda and may has similar effects on the human population located around the dam.

Keywords

• Key words: Clastogenic effects, Rana ridibunda, environmental genotoxicity, micronucleus test, Tandaha dam, peripheral erythrocytes.

References

1. Agrawal, R. C. (1999). Induction of chromosomal aberrations by propoxur in mouse bone marrow cells. Biomed. Environ. Sci. 12: 292-295.
2. Al-Sabti, K. (1986). Comparative micronucleated erythrocyte cell induction in three cyprinids by five carcinogenic-mutagenic chemicals. Cytobios 47: 147- 154.
3. Al-Sabti, K. (1995). An in vitro binucleated blocked hepatic cell technique for genotoxicity testing in fish. Mutat. Res. 335: 109-120.
4. Al-Sabti, K. and Metcalfe, C.D. (1995). Fish micronuclei for assessing genotoxicity in water. Mutat. Res. 343: 121-135.
5. Andrade, V.M., Silva, J., Silva, F.R., Heuser, V.D., Dias, J.F., Yoneama, M.L. and Freitas, T.R. (2004). Fish as bioindicators to assess the effects of pollution in two southern Brazilian rivers using the Comet assay and micronucleus test. Environ. Mol. Mutagen. 44: 459-468.
6. Bhalli, J.A., Khan, Q.M., Haq, M.A., Khalid, A.M. and Nasim, A. (2006). Cytogenetic analysis of Pakistani individuals occupationally exposed to pesticides in a pesticide production industry. Mutagenesis 21: 143-148.
7. Bhunya, S.P. and Jena, G.B. (1993). Studies on the genotoxicity of monocrotophos, an organophosphate insecticide, in the chick in vivo test system. Mutat. Res. 292: 231-239.
8. Çelikler, S., Yildiz, G., Vatan, O. and Bilaloğlu, R (2008). In vitro antigenotoxicity of Ulva rigida C. Agardh (Chlorophyceae) extract against induction of chromosome aberration, sister chromatid exchange and micronuclei by mutagenic agent MMC. Biomed. Environ. Sci. 21:492-498.

9. Fernandez, M. and L'Haridon, J. (1992). Influence of lighting conditions on toxicity and genotoxicity of various PAH in the newt in vivo. Mutat Res 298: 31-41.
10. Hayashi, M., Ueda, T., Uyeno, K., Wada, K., Kinae, N., Saotome, K., Tanaka, N., Takai, A., Sasaki, Y.F., Asano, N., Sofuni, T. and Ojima, Y. (1998). Development of genotoxicity assay systems that use aquatic organisms. Mutat. Res. 399:125-33.
11. Heddle, J.A. (1973). A rapid in vivo test for chromosomal damage. Mutation. Res. 18: 187-190.
12. Heddle, J. A., Salamone, M.F., Hite, M., Kirkhart, B., Mavournin, K., MacGregor, J.G. and Newell, G.W. (1983). The induction of micronuclei as a measure of genotoxicity. Mutation. Res. 123:61-118.
13. Heddle, J.A. (1990). Micronuclei in vivo. Prog. Clin. Biol. Res. 340: 185-194.
14. Heddle, J.A., Cimino, M.C., Hayashi, M., Romagna, F., Shelby, M.D., Tucker, J.D., Vanparys, P. and MacGregor, J.T. (1991). Micronuclei as an index of cytogenetic damage: past, present and future. Environ. Mol. Mutagen. 18: 277- 291.
15. Konopacka, M., Widel, M. and Rzeszowska-Wolny, J. (1998). Modifying effect of vitamins C, E and beta-carotene against gamma-ray-induced DNA damage in mouse cells. Mutat. Res. 417 :85-94.
16. Krishna, G., Kropko, M.L., Ciaravino, V. and Theiss, J.C. (1991). Simultaneous micronucleus and chromosome aberration assessment in the rat. Mutat. Res. 264: 29-35.
17. Lajmanovich, R.C., Cabagna, M., Peltzer, P.M., Stringhini, G.A., and Attademo, A.M. (2005). Micronucleus induction in erythrocytes of the Hyla pulchella tadpoles (Amphibia: Hylidae) exposed to insecticide endosulfan. Mutat. Res. 587: 67-72.
18. Lyne, T.B.B.J., Lamb, T. and Gibbons, J.W. (1992). The application of bioassays in risk assessment of environmental pollution. Risk Anal. 12: 361-365. Ma, T.H., Harris, M.M., Anderson, V.A., Ahmed, I., Mohammad, K., Bare, J.L. and Lin, G. (1984). Tradescantia-Micronucleus (Trad-MCN) tests on 140 health-related agents. Mutat. Res. 138: 157-167.
19. Ma, T.H., Cabrera, G.L. and Owens, E. (2005). Genotoxic agents detected by plant bioassays. Rev. Environ. Health 20:1-13.
20. Marques, S.M., Antunes, S.C., Pissarra, H., Pereira, M.L., Goncalves, F. and Pereira, R. (2009). Histopathological changes and erythrocytic nuclear abnormalities in Iberian green frogs (Rana perezi Seoane) from a uranium mine pond. Aquat. Toxicol. 91: 187-195.
21. Orhan, G., Ekmekci, A. and Menevse, S. (1993). The effect of the male contraceptive agent gossypol acetic acid on mouse bone marrow cells in vivo: micronuclei and mitotic index. Contraception 47: 377-385.
22. Saleh, K. and Zeytinoğlu, H. (2001). Micronucleus test in peripheral erythrocytes of Rana ridipunda as an indicator of environmental pollution. Ana. Uni. J. Sci. and Tech. 2:77-82.
23. Saleh, K. and Sarhan, M. (2007). Clastogenic analysis of chicken farms using micronucleus test in peripheral blood. J. Applied Sci. Res. 3: 1646-1649.
24. Schmid, W. (1975). The micronucleus test. Mutat. Res. 31: 9-15.
25. Siboulet, R., Grinfeld, P. and Jaylet, A. (1984). Micronuclei in red blood cells of the new Pleurodeles watl Michah: induction with X-rays and chemicals. Mutat. Res. 125: 275-28.
26. Villarini, M., Moretti, M., Pasquini, R., Scassellati-Sforzolini, G., Fatigoni, C., Marcarelli, M., Monarca, S. and Rodriguez, A.V. (1998). In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage ('comet' assay) in relation to the induction of sister-chromatid exchanges and micronuclei. Toxicology 130: 129-139.
27. Villela IV, D.O.I., Da Silva, J. and Henrigues, J.A. (2006). DNA damage and repair in haemolymph cells of golden mussel exposed to environmental contaminants. Mutat. Res. 605: 78-86.
28. Yamamoto, K.I. and Kikuchi, Y. (1980). A comparison of diameters of micronuclei induced by clastogens and by spindle poisons. Mutation. Res. 71: 127-131.
29. Zhuleva, L., Umnova, N.V. and Rumak, V.S. (1996). Detection of micronuclei in desquamating cells of the human oral mucosa in the territory of South Vietnam. Genetika 32: 1700-1704.
30. Zuniga, G., Torres-Bugarin, O., Ramirez-Munoz, M.P., Ramos, A., Fanti- Rodriguez, E., Portilla, E., Garcia-Martinez, D., Cantu, J.M., Gallegos-Arreola, M.P. and Sanchez-Corona, J. (1996). Spontaneous micronuclei in peripheral blood erythrocytes from 35 mammalian species. Mutat. Res. 369:123-127.