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Cell-wall images of marine algae by metachromatic method

Year 2015, Volume: 21 Issue: 1, 12 - 22, 20.09.2015

Hüseyin Erdugan Kasım Cemal Güven Burak Coban Rıza Akgül

Abstract

Abstract Metachromatic image of cell-walls were investigated for seven algae. Species; red: Acanthophora nayadiformis , Chondria capillaris , Phyllophora crispa , brown: Petalonia fascia , Cystoseira barbata , Dictyota dichotoma and green: Ulva linza . The tested methachromatic dyes were azur II, methylene blue and thioflavine. In this work, locations of algal acidic and sulfated polysaccharides in cell-wall of red and brown algae were demonstrated by metachromasy , except in green algae. The metachromatic method clearly showed the cell wall constituents of algae. Cuticle and epidermal cells intensely stained but also the inner cell wall polysaccharide accumulation clearly shown. The most obvious staining and color change was seen by azur II.

Keywords

Keywords: Metachromasy algae cell wall components acidic polysaccharides

References

  • Akatsuka, J., Iwamoto, K. (1979) Histochemical localization of agar and cellulose in the tissue of Gelidium pacificum (Gelidiacea, Rhodophyta). Bot. Mar. 22: 367-370.
  • Anderson, N.S., Dolan, T.C.S., Rees, D.A. (1965) Evidence for a common structural pattern in the polysaccharide sulphates of the Rhodphyceae. Nature 205: 1060-1062.
  • Ando, Y. (1955) Methachromasia in the mucilaginous cells of Laminariaceae.
  • Igaku to Seibutsugaku 37: 21-25. Andrade, L.R., Salgado, L.T., Farina, M., Pereira, M.S., Mourão, P.A.S., Filho, G.M.A. (2004) Ultrastructure of acidic polysaccharides from the cell walls of brown algae. J. Struct. Biol. 145 (3): 216-225.
  • Balkan, G., Coban, B., Guven, K.C. (2005) Fractionation of agarose and Gracilaria verrucosa agar and comparison of their IR spectra with different agar. Acta Pharm. Turcica 47: 93-106.
  • Bank, O., Bungenberg de Jong, H.G. (1939) Untersuchungen über
  • Metachromasie. Protoplasma 32: 489. Barnes, H. (2005) Oceanography and Marine Biology. An Annual Review.
  • Scotland Aberdeen University Pres. 26: 221-273. Chiovitti, A., Bacic, A., Craik, D.J., Munro, S.L.A., Kraft, G.T., Liao, M.L. (1997) Cell-wall polysaccharides from Australian red algae of the family
  • Solieriaceae (Gigartinales, Rhodophyta): novel, highly pyruvated carrageenans from the genus Callophycus. Carbohyd. Res. 299: 229-243. Cronshaw, J., Myers, A., Preston, R.D. (1958) A chemical and physical investigation of the cell walls of some marine algae. Biochim. Biophys. Acta. 27: 89
  • Ehrlich, P. (1887) Beitrage zur Kenntniss der Anilinfarbungen und ihrer
  • Verwendung in der Mikroskopischen Tecknik. Archiv. P. Mikrosk. 13: 263-277. Evans, L.V., Holligan, M.S. (1972) Correlated light and electron microscope studies on brown algae. New Phytol. 71: 1161-1172.
  • Frei, F., Preston, R.D. (1961) Variants in the structural polysaccharides of algal cell walls. Nature 192: 939-943.
  • Frei, F., Preston, R.D. (1962) Configuration of alginic acid in marine brown algae. Nature 196: 130-134.
  • Gordon-Mills, E.M., McCandless, E.L. (1975) Carrageenans in the cell walls of
  • Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody. Phycologia 14: 275-281. Gretz, M.R., Aronson, J.M., Sommerfeld, N.R. (1980) Cellulose in the cell walls of the Bangiophyceae (Rhodophyta). Science 207: 779-781.
  • Guiry, M.D., Guiry, G.M. (2014) AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org (Accessed 02 May 2014).
  • Guven, K.C., Coban, B. (2013) LAS pollution of the Sea of Marmara, Golden
  • Horn and Istanbul Strait (Bosphorus) during 2004-2007. J. Black Sea/Med. Environ. 19: 331-354.
  • Guven, K.C., Ertan, G. (1984) The correlation equations for the assays of heparin in metachromatic and thrombin time methods. Thromb. Res. 33(2): 197- 20
  • Guven, K.C., Gurakin, N., Akıncı, S., Bektas, A., Oz, V. (1994) Metachromatic method for the assay of LAS in town and sea water. Acta Pharm. Turc. 36: 136
  • Guven, K.C., Guvener, B. (1985a) A metachromatic method for identification of alginic acid, agar and carragenen. Fette. Seifen. Antrichmittel 87: 172-176.
  • Guven, K.C., Guvener, B. (1985b) Metachromatic identification of (iota-, kappa-, lambda-) carragenens. Bot. Mar. 28: 221-222.
  • Guven, K.C., Ozsoy, Y., Ozturk, B., Topaloglu B., Ulutin O.N. (1991) Raparin a new heparinoid from Rapana venosa Valenciennes. Die Pharmazie 46: 547- 5
  • Guvener, B., Guven, K.C., Peremeci, E. (1988) A metachromatic assay method for carrageenans in raw material and in tooth pastes. Sci. Pharm. 56: 283-288.
  • Haslin, C., Lahaye, M., Pellegrini, M. (2000) Chemical composition and structure of sulphated water-soluble cell-wall polysaccharides from the gametic, carposporic and tetrasporic stages of Asparagopsis armata Harvey
  • (Rhodophyta, Bonnemaisoniaceae). Bot. Mar. 43: 475-482. Ji, M., Hon Beng, G.W., Fan, X. (1996) Studies on the structural features of sulfated of galactan polysaccharides from some red seaweeds using 13-6 NMR spectroscopy. Hiyong Yu Muzhau. 27: 330-335.
  • Jones, J. (1930) An investigation into the bacterial associations of some
  • Cyanophyceae. Ann. Bot. 44: 721-740. Jones, J.K.N. (1950) The structure of the mannan present in Porphyra umbilicalis. J. Chem. Soc. 1950: 3292-3295.
  • Jorpes, E.J. (1939) Heparin Oxford University Press, London.
  • Kıran, E., Teksoy, I., Guven, K.C., Guler, E., Guner, H. (1980) Studies on seaweeds for paper production. Bot. Mar. 23: 205-208.
  • Kreger, D.R. (1962) Physiology and Biochemistry of Algae (ed., R. A. Lewin), Academic, New York, pp. 315-335.
  • Kylin, H. (1915) Untersuchungen über die Biochemie der Meeresalgen. Hoppe
  • Seyler’s. Z. physiol. Chem. 94: 337-429. La Claire, J.W., Dawes, C.J. (1976) An autoradiographic and histochemical localization of sulphated polysaccharides in Euchema nudum (Rhodophyta). J. Phycol. 12: 368-375.
  • Lison, L. (1935) Etudes sur la metachromasie colorants metachromatiques et substances chromotropes. Arch. Biol. 46: 599-668.
  • McCandless, E.L., West, J.A., Wollmer, C.M. (1981) Carrageenans of species in the genus Phyllophora. XII. Int. Seaweed Sypmosium, Walter de Gruyter Co., Berlin, Newyork, pp.473-478.
  • McCully, M.E. (1966) Histological studies on the genus Fucus. I. Light microscopy of the mature vegetative plant. Protoplasma 42: 287-305.
  • McHugh, D.J. (2003) A guide to the seaweed industry, FAO Fisheries Technical Paper 441. FAO, Rome.
  • Mirande, R. (1913) Sur la composition chimique de la membrane et le morcellement du thalle che les Siphonales. Ann. Sci. Nat. Botan. et biol. Vegetale. 18: 147-264.
  • Naylor, G., Russell-Wells, B. (1934) On the presence of cellulose and its distribution in the cell walls of brown and red algae. Ann. Bot. (London) 48: 635-6
  • Nicolai, E., Preston, R.D. (1952) Cell wall studies in the Chlorophyceae. I. A general survey of submicroscopic structure in filamentous species. Proc. Roy.
  • Soc. B. pp. 140-244. DOI: 10.1098/rspb.1952.0061. Published 16 October 1952.
  • Okamura, K., Oishi, Y. (1905) Structure and agar localization in Gelidium amansii Lamx. Suisan Kosyuzyo Shiken Hokoku 3: 93-104.
  • Pal, M.K., Schubert, M. (1962) Measurement of the stability of metachromatic compounds. J. Am. Chem. Soc. 84: 4384-4393.
  • Parker, B.C., Diboll, A.G. (1966) Alcian stains for histochemical localization of acid and sulfated polysaccharides in algae. Phycologia 6: 37-46.
  • Peats, S. (1981) The infrared spectra of carrageenans extracted from various algae. Xth International Seaweed Symposium (Goteborg Aug. 11-15.1980) (Ed., T. Lewring), Walter de Gruyter, Berlin, pp. 495-507.
  • Ray, B., Lahaye, M. (1995) Cell-wall polysaccharides from the marine green alga Ulva rigida (Ulvales, Chlorophyta). Extraction and chemical composition. Carbohydr. Res. 274: 251–261.
  • Sakai, S., Ueta, Y., Terauchi, Y. (1993) Band gap energy and band lineup of III
  • V alloy semiconductors incorporating nitrogen and boron. Japn. J. Appl. Phys. 32: 4413-4417.
  • Senni, K., Gueniche, F., Foucault-Bertaud, A., Igondjo-Tchen, S., Fioretti, F., Colliec-Jouault, S., Durand, P., Guezennec, J., Godeau, G., Letourneur, D. (2006) Fucoidan a sulfated polysaccharide from brown algae is a potent modulator of connective tissue proteolysis. Arch. Biochem. Biophys. 445(1): 56
  • Soedjak, H.S. (1994) Colorimetric determination of carrageenans and other anionic hydrocolloids with methylene blue. Anal. Chem. 66: 4514-4518.
  • Stone, A.L., Childers, L.G., Bradley, D.F. (1963) Investigation of structural aspects and classification of plant sulfated polysaccharides on the basis of the optical properties of their complexes with metachromatic dyes. Biopolymers 1: 11
  • Thompson, E.W., Preston, R.D. (1967) Proteins in the cell walls of some green algae (Nitella opaca, Cladophora, Chaetomorpha, Codium). Nature 213: 648- 6
  • Usov, A.I. (1984) NMR spectroscopy of red seaweed polysaccharides: agars, carrageenans, and xylans. Bot. Mar. 27: 189-202.
  • Yamagaki, T., Maeda, M., Kanazawa, K., Ishizuka, Y., Nakanishi, H. (1997)
  • Structural clarification of Caulerpa cell wall β-1.3-xylan by NMR spectroscopy. Biosci. Biotechnol. Biochem 61: 1077-1080.

Year 2015, Volume: 21 Issue: 1, 12 - 22, 20.09.2015

Hüseyin Erdugan Kasım Cemal Güven Burak Coban Rıza Akgül

Abstract

References

  • Akatsuka, J., Iwamoto, K. (1979) Histochemical localization of agar and cellulose in the tissue of Gelidium pacificum (Gelidiacea, Rhodophyta). Bot. Mar. 22: 367-370.
  • Anderson, N.S., Dolan, T.C.S., Rees, D.A. (1965) Evidence for a common structural pattern in the polysaccharide sulphates of the Rhodphyceae. Nature 205: 1060-1062.
  • Ando, Y. (1955) Methachromasia in the mucilaginous cells of Laminariaceae.
  • Igaku to Seibutsugaku 37: 21-25. Andrade, L.R., Salgado, L.T., Farina, M., Pereira, M.S., Mourão, P.A.S., Filho, G.M.A. (2004) Ultrastructure of acidic polysaccharides from the cell walls of brown algae. J. Struct. Biol. 145 (3): 216-225.
  • Balkan, G., Coban, B., Guven, K.C. (2005) Fractionation of agarose and Gracilaria verrucosa agar and comparison of their IR spectra with different agar. Acta Pharm. Turcica 47: 93-106.
  • Bank, O., Bungenberg de Jong, H.G. (1939) Untersuchungen über
  • Metachromasie. Protoplasma 32: 489. Barnes, H. (2005) Oceanography and Marine Biology. An Annual Review.
  • Scotland Aberdeen University Pres. 26: 221-273. Chiovitti, A., Bacic, A., Craik, D.J., Munro, S.L.A., Kraft, G.T., Liao, M.L. (1997) Cell-wall polysaccharides from Australian red algae of the family
  • Solieriaceae (Gigartinales, Rhodophyta): novel, highly pyruvated carrageenans from the genus Callophycus. Carbohyd. Res. 299: 229-243. Cronshaw, J., Myers, A., Preston, R.D. (1958) A chemical and physical investigation of the cell walls of some marine algae. Biochim. Biophys. Acta. 27: 89
  • Ehrlich, P. (1887) Beitrage zur Kenntniss der Anilinfarbungen und ihrer
  • Verwendung in der Mikroskopischen Tecknik. Archiv. P. Mikrosk. 13: 263-277. Evans, L.V., Holligan, M.S. (1972) Correlated light and electron microscope studies on brown algae. New Phytol. 71: 1161-1172.
  • Frei, F., Preston, R.D. (1961) Variants in the structural polysaccharides of algal cell walls. Nature 192: 939-943.
  • Frei, F., Preston, R.D. (1962) Configuration of alginic acid in marine brown algae. Nature 196: 130-134.
  • Gordon-Mills, E.M., McCandless, E.L. (1975) Carrageenans in the cell walls of
  • Chondrus crispus Stack. (Rhodophyceae, Gigartinales). I. Localization with fluorescent antibody. Phycologia 14: 275-281. Gretz, M.R., Aronson, J.M., Sommerfeld, N.R. (1980) Cellulose in the cell walls of the Bangiophyceae (Rhodophyta). Science 207: 779-781.
  • Guiry, M.D., Guiry, G.M. (2014) AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org (Accessed 02 May 2014).
  • Guven, K.C., Coban, B. (2013) LAS pollution of the Sea of Marmara, Golden
  • Horn and Istanbul Strait (Bosphorus) during 2004-2007. J. Black Sea/Med. Environ. 19: 331-354.
  • Guven, K.C., Ertan, G. (1984) The correlation equations for the assays of heparin in metachromatic and thrombin time methods. Thromb. Res. 33(2): 197- 20
  • Guven, K.C., Gurakin, N., Akıncı, S., Bektas, A., Oz, V. (1994) Metachromatic method for the assay of LAS in town and sea water. Acta Pharm. Turc. 36: 136
  • Guven, K.C., Guvener, B. (1985a) A metachromatic method for identification of alginic acid, agar and carragenen. Fette. Seifen. Antrichmittel 87: 172-176.
  • Guven, K.C., Guvener, B. (1985b) Metachromatic identification of (iota-, kappa-, lambda-) carragenens. Bot. Mar. 28: 221-222.
  • Guven, K.C., Ozsoy, Y., Ozturk, B., Topaloglu B., Ulutin O.N. (1991) Raparin a new heparinoid from Rapana venosa Valenciennes. Die Pharmazie 46: 547- 5
  • Guvener, B., Guven, K.C., Peremeci, E. (1988) A metachromatic assay method for carrageenans in raw material and in tooth pastes. Sci. Pharm. 56: 283-288.
  • Haslin, C., Lahaye, M., Pellegrini, M. (2000) Chemical composition and structure of sulphated water-soluble cell-wall polysaccharides from the gametic, carposporic and tetrasporic stages of Asparagopsis armata Harvey
  • (Rhodophyta, Bonnemaisoniaceae). Bot. Mar. 43: 475-482. Ji, M., Hon Beng, G.W., Fan, X. (1996) Studies on the structural features of sulfated of galactan polysaccharides from some red seaweeds using 13-6 NMR spectroscopy. Hiyong Yu Muzhau. 27: 330-335.
  • Jones, J. (1930) An investigation into the bacterial associations of some
  • Cyanophyceae. Ann. Bot. 44: 721-740. Jones, J.K.N. (1950) The structure of the mannan present in Porphyra umbilicalis. J. Chem. Soc. 1950: 3292-3295.
  • Jorpes, E.J. (1939) Heparin Oxford University Press, London.
  • Kıran, E., Teksoy, I., Guven, K.C., Guler, E., Guner, H. (1980) Studies on seaweeds for paper production. Bot. Mar. 23: 205-208.
  • Kreger, D.R. (1962) Physiology and Biochemistry of Algae (ed., R. A. Lewin), Academic, New York, pp. 315-335.
  • Kylin, H. (1915) Untersuchungen über die Biochemie der Meeresalgen. Hoppe
  • Seyler’s. Z. physiol. Chem. 94: 337-429. La Claire, J.W., Dawes, C.J. (1976) An autoradiographic and histochemical localization of sulphated polysaccharides in Euchema nudum (Rhodophyta). J. Phycol. 12: 368-375.
  • Lison, L. (1935) Etudes sur la metachromasie colorants metachromatiques et substances chromotropes. Arch. Biol. 46: 599-668.
  • McCandless, E.L., West, J.A., Wollmer, C.M. (1981) Carrageenans of species in the genus Phyllophora. XII. Int. Seaweed Sypmosium, Walter de Gruyter Co., Berlin, Newyork, pp.473-478.
  • McCully, M.E. (1966) Histological studies on the genus Fucus. I. Light microscopy of the mature vegetative plant. Protoplasma 42: 287-305.
  • McHugh, D.J. (2003) A guide to the seaweed industry, FAO Fisheries Technical Paper 441. FAO, Rome.
  • Mirande, R. (1913) Sur la composition chimique de la membrane et le morcellement du thalle che les Siphonales. Ann. Sci. Nat. Botan. et biol. Vegetale. 18: 147-264.
  • Naylor, G., Russell-Wells, B. (1934) On the presence of cellulose and its distribution in the cell walls of brown and red algae. Ann. Bot. (London) 48: 635-6
  • Nicolai, E., Preston, R.D. (1952) Cell wall studies in the Chlorophyceae. I. A general survey of submicroscopic structure in filamentous species. Proc. Roy.
  • Soc. B. pp. 140-244. DOI: 10.1098/rspb.1952.0061. Published 16 October 1952.
  • Okamura, K., Oishi, Y. (1905) Structure and agar localization in Gelidium amansii Lamx. Suisan Kosyuzyo Shiken Hokoku 3: 93-104.
  • Pal, M.K., Schubert, M. (1962) Measurement of the stability of metachromatic compounds. J. Am. Chem. Soc. 84: 4384-4393.
  • Parker, B.C., Diboll, A.G. (1966) Alcian stains for histochemical localization of acid and sulfated polysaccharides in algae. Phycologia 6: 37-46.
  • Peats, S. (1981) The infrared spectra of carrageenans extracted from various algae. Xth International Seaweed Symposium (Goteborg Aug. 11-15.1980) (Ed., T. Lewring), Walter de Gruyter, Berlin, pp. 495-507.
  • Ray, B., Lahaye, M. (1995) Cell-wall polysaccharides from the marine green alga Ulva rigida (Ulvales, Chlorophyta). Extraction and chemical composition. Carbohydr. Res. 274: 251–261.
  • Sakai, S., Ueta, Y., Terauchi, Y. (1993) Band gap energy and band lineup of III
  • V alloy semiconductors incorporating nitrogen and boron. Japn. J. Appl. Phys. 32: 4413-4417.
  • Senni, K., Gueniche, F., Foucault-Bertaud, A., Igondjo-Tchen, S., Fioretti, F., Colliec-Jouault, S., Durand, P., Guezennec, J., Godeau, G., Letourneur, D. (2006) Fucoidan a sulfated polysaccharide from brown algae is a potent modulator of connective tissue proteolysis. Arch. Biochem. Biophys. 445(1): 56
  • Soedjak, H.S. (1994) Colorimetric determination of carrageenans and other anionic hydrocolloids with methylene blue. Anal. Chem. 66: 4514-4518.
  • Stone, A.L., Childers, L.G., Bradley, D.F. (1963) Investigation of structural aspects and classification of plant sulfated polysaccharides on the basis of the optical properties of their complexes with metachromatic dyes. Biopolymers 1: 11
  • Thompson, E.W., Preston, R.D. (1967) Proteins in the cell walls of some green algae (Nitella opaca, Cladophora, Chaetomorpha, Codium). Nature 213: 648- 6
  • Usov, A.I. (1984) NMR spectroscopy of red seaweed polysaccharides: agars, carrageenans, and xylans. Bot. Mar. 27: 189-202.
  • Yamagaki, T., Maeda, M., Kanazawa, K., Ishizuka, Y., Nakanishi, H. (1997)
  • Structural clarification of Caulerpa cell wall β-1.3-xylan by NMR spectroscopy. Biosci. Biotechnol. Biochem 61: 1077-1080.
There are 55 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Hüseyin Erdugan This is me

Kasım Cemal Güven This is me

Burak Coban This is me

Rıza Akgül This is me

Publication Date September 20, 2015
Published in Issue Year 2015 Volume: 21 Issue: 1

Cite

APA Erdugan, H. ., Güven, K. C. ., Coban, B. ., Akgül, R. . (2015). Cell-wall images of marine algae by metachromatic method. Journal of Black Sea / Mediterranean Environment, 21(1), 12-22.
AMA Erdugan H, Güven KC, Coban B, Akgül R. Cell-wall images of marine algae by metachromatic method. Journal of Black Sea / Mediterranean Environment. April 2015;21(1):12-22.
Chicago Erdugan, Hüseyin, Kasım Cemal Güven, Burak Coban, and Rıza Akgül. “Cell-Wall Images of Marine Algae by Metachromatic Method”. Journal of Black Sea / Mediterranean Environment 21, no. 1 (April 2015): 12-22.
EndNote Erdugan H, Güven KC, Coban B, Akgül R (April 1, 2015) Cell-wall images of marine algae by metachromatic method. Journal of Black Sea / Mediterranean Environment 21 1 12–22.
IEEE H. . Erdugan, K. C. . Güven, B. . Coban, and R. . Akgül, “Cell-wall images of marine algae by metachromatic method”, Journal of Black Sea / Mediterranean Environment, vol. 21, no. 1, pp. 12–22, 2015.
ISNAD Erdugan, Hüseyin et al. “Cell-Wall Images of Marine Algae by Metachromatic Method”. Journal of Black Sea / Mediterranean Environment 21/1 (April 2015), 12-22.
JAMA Erdugan H, Güven KC, Coban B, Akgül R. Cell-wall images of marine algae by metachromatic method. Journal of Black Sea / Mediterranean Environment. 2015;21:12–22.
MLA Erdugan, Hüseyin et al. “Cell-Wall Images of Marine Algae by Metachromatic Method”. Journal of Black Sea / Mediterranean Environment, vol. 21, no. 1, 2015, pp. 12-22.
Vancouver Erdugan H, Güven KC, Coban B, Akgül R. Cell-wall images of marine algae by metachromatic method. Journal of Black Sea / Mediterranean Environment. 2015;21(1):12-2.