BibTex RIS Cite

HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast

Year 2013, Volume: 19 Issue: 1, 58 - 69, 01.04.2013

Abstract

Abstract In this article, homogeneous liquid-liquid extraction (HOLLE), combined with HPLC-fluorescence detector (HPLC-FLD and Fluorescence Spectrophotometer, has been developed for the extraction and determination of polycyclic aromatic hydrocarbons (PAHs) in polluted seawater. A liquid-liquid extraction followed by evaporative concentration method was used to determine the concentration of polycyclic aromatic hydrocarbons (PAH) in Lebanese marine water. Hydrocarbons were extracted from 450 ml of water into 75 ml n-hexane and then concentrated into 1 or 2 ml solution by evaporation. Internal standard addition method was used to determine the pyrene. Under the optimum conditions, repeatability was carried out by spiking pyrene at concentration level of 114.5 μg/l. the RSDs varied between 1.1 and 8.5% (n = 3). The LODs, based on S/N of 3, ranged from 0.025 to 0.25 μg/l. The pyrene determined in marine water was generated from the incomplete combustion of fuel oil in the powerplant of Zouk Mikael (Lebanon). Several PAHs were observed but the pyrene was easily indentified and quantified, due to the relative high concentration of this compound.

References

  • Barranco, A., Alonso-Salces, R. M., Bakkali, A., Berrueta, L. A., Gallo, B., Vicente, F. (2003) Solid-phase clean-up in the liquid chromatographic determination of polycyclic aromatic hydrocarbons in edible oils. J. Chromatogr. A 988: 33-40.
  • Berset, J. D., Ejem, M., Holzer, R., Lischer, P. (1999) Comparison of different drying, extraction and detection techniques for the determination of priority polycyclic aromatic hydrocarbons in background contaminated soil samples. Anal. Chim. Acta. 383: 263-275.
  • Brouwer, E. R., Hermans, A. N. J., Lingeman, H., Brinkman, U. A. T. (1994)
  • El-Deeb, M. K. Z., Emara, I. H. (2005) Polycyclic aromatic hydrocarbons and aromatic plasticizer materials in the seawater of Alexandria coastal area.
  • Egyptian Journal of Aquatic Research 31: 15-24. Filipkowska, A., Lubecki, L., Kowalewska, G. (2005) Polycyclic aromatic hydrocarbon analysis in different matrices of the marine environment. Analytica Chimica Acta 547: 243-254.
  • Giessing, A. M. B., Mayer, L. M., Forbes, T. L. (2003) Synchronous fluorescence spectrometry of 1-hydroxypyrene: a rapid screening method for identification of PAH exposure in tissue from marine polychaetes. Mar. Environ. Res. 56: 599-615.
  • Harrak, R. El., Calull, M., Marce, R. M., Borrull, F. (1996) Determination of polycyclic aromatic hydrocarbons (PAH’s) in water by solid-phase extraction membranes. Int. J. Environ. Anal. Chem. 64: 47-57.
  • Kaag, N. H. B. M., Foekema, E. M., Scholten, M. C. Th., Van Straalen, N. M. (1997) Comparison of contaminant accumulation in three species of marine invertebrates with different feeding habits. Environ. Toxicol. Chem. 16: 837- 8
  • Kanaki, M., Nikolaou, A., Makri, C. A., Lekkas, D. F. (2007) The occurrence of priority PAHs, nonylphenol and octylphenol in inland and coastal waters of central Greece and the island of Lesvos. Desalination 210: 16-23.
  • Kishikawa, N., Wada, M., Kuroda, N., Akiyama, S., Nakashima, K. (2003)
  • Lacorte, S., Guiffard, I., Fraisse, D., Barcelo, D. (2000) Broad spectrum analysis of 109 priority compounds listed in the 76/464/EEC council directive using solid-phase extraction and GC/EI/MS. Anal. Chem. 72 (7): 1430-1440.
  • Lors, C., Perie, F., Grand, C., Damidot, D. (2009) Benefits of ecotoxicological bioassays in the evaluation of a filed biotreatment of PAHs polluted soils.
  • Global NEST Journal 11 (3): 251-259. Miẻge, C., Bouzige, M., Nicol, S., Dugary, J., Pichon, V., Hennion, M. C. (1998) Selective immunoclean-up followed by liquid or gas chromatography for the monitoring of polycyclic aromatic hydrocarbons in urban waste water and sewage sludges used for soil amendment. J. Chromatogr. A 859 (1): 29-39.
  • Muel, B., Lacroix, G. (1960) Analyses des hydrocarbures aromatiques polynucléaires dans l’air. Bulletin de la société chimique de France 11-12: 2139-2147.
  • Nirmaier, H. P., Fisher, E., Meyer, A., Henze, G. (1996) Determination of polycyclic aromatic hydrocarbons in water samples using high performance liquid chromatography with amperometric detection. J. Chromatogr. A 730: 169-1
  • Obana, H., Hori, S., Nakamura, A., Kashimoto, T. (1983) Uptake and release of polynuclear aromatic hydrocarbons by short-necked clams (Tapes japonica). Water Res. 17: 1183-1187.
  • Pino, V., Ayala, J. H., Afonso, A. M., González, V. (2002) Determination of polyaromatic hydrocarbons in seawater by high-performance liquid chromatographywith fluorescence detection following micelle-mediated preconcentration. J. Chromatgr. A 949: 291-299.
  • Rima, J., Lamotte, M., Joussot-Dubien, J. (1982) Application of the shpolskii effect to quantitative analysis of monomethylphenanthrene isomers. Analytical Chemistry 54: 1059-1070.
  • Shariati-Feizabadi, Sh., Yamini, Y., Bahramifar, N. (2003) Determination of some polycyclic aromatic hydrocarbons (PAHs) from aqueous solutions. Anal. Chim. Acta 489 (1): 21-31.
  • Vagi, M. C., Kostopoulou, M. N., Petsas, A. S., Lalousi, M. E., Rasouli, Ch, Lekkas, T. D. (2005) Toxicity of organophoshorous pesticides to the marine alga Tetraselmis suecica. Global NEST Journal 7 (2): 222-227.
  • Varanasi, U., Reichert, W. L., Stein, J. E., Brown, D. W., Sanborn, H. R. (1985)
  • Wise, S. A., Sander, L. C., May, W. E. (1993) Determination of polycyclic aromatic hydrocarbons by liquid chromatography. J. Chromatogr. A 642 (1-2): 329-3
  • Wolska, L., Galer, K., Gỏrecki, T., Namiesnik, J. (1999) Surface water procedure for chromatographic determination for polycyclic aromatic hydrocarbons and polychlorinated biphenyl. Talanta 50 (5): 985-991.
  • Zaghden, H., Kallel, M., Elleuch, B., Oudot, J., Saliot, A. (2007) Sources and distribution of aliphatic and polyaromatic hydrocarbons in sediments of Sfax,
  • Tunisia, Mediterranean Sea. Marine Chemistry 105 (1-2): 70-89.
Year 2013, Volume: 19 Issue: 1, 58 - 69, 01.04.2013

Abstract

References

  • Barranco, A., Alonso-Salces, R. M., Bakkali, A., Berrueta, L. A., Gallo, B., Vicente, F. (2003) Solid-phase clean-up in the liquid chromatographic determination of polycyclic aromatic hydrocarbons in edible oils. J. Chromatogr. A 988: 33-40.
  • Berset, J. D., Ejem, M., Holzer, R., Lischer, P. (1999) Comparison of different drying, extraction and detection techniques for the determination of priority polycyclic aromatic hydrocarbons in background contaminated soil samples. Anal. Chim. Acta. 383: 263-275.
  • Brouwer, E. R., Hermans, A. N. J., Lingeman, H., Brinkman, U. A. T. (1994)
  • El-Deeb, M. K. Z., Emara, I. H. (2005) Polycyclic aromatic hydrocarbons and aromatic plasticizer materials in the seawater of Alexandria coastal area.
  • Egyptian Journal of Aquatic Research 31: 15-24. Filipkowska, A., Lubecki, L., Kowalewska, G. (2005) Polycyclic aromatic hydrocarbon analysis in different matrices of the marine environment. Analytica Chimica Acta 547: 243-254.
  • Giessing, A. M. B., Mayer, L. M., Forbes, T. L. (2003) Synchronous fluorescence spectrometry of 1-hydroxypyrene: a rapid screening method for identification of PAH exposure in tissue from marine polychaetes. Mar. Environ. Res. 56: 599-615.
  • Harrak, R. El., Calull, M., Marce, R. M., Borrull, F. (1996) Determination of polycyclic aromatic hydrocarbons (PAH’s) in water by solid-phase extraction membranes. Int. J. Environ. Anal. Chem. 64: 47-57.
  • Kaag, N. H. B. M., Foekema, E. M., Scholten, M. C. Th., Van Straalen, N. M. (1997) Comparison of contaminant accumulation in three species of marine invertebrates with different feeding habits. Environ. Toxicol. Chem. 16: 837- 8
  • Kanaki, M., Nikolaou, A., Makri, C. A., Lekkas, D. F. (2007) The occurrence of priority PAHs, nonylphenol and octylphenol in inland and coastal waters of central Greece and the island of Lesvos. Desalination 210: 16-23.
  • Kishikawa, N., Wada, M., Kuroda, N., Akiyama, S., Nakashima, K. (2003)
  • Lacorte, S., Guiffard, I., Fraisse, D., Barcelo, D. (2000) Broad spectrum analysis of 109 priority compounds listed in the 76/464/EEC council directive using solid-phase extraction and GC/EI/MS. Anal. Chem. 72 (7): 1430-1440.
  • Lors, C., Perie, F., Grand, C., Damidot, D. (2009) Benefits of ecotoxicological bioassays in the evaluation of a filed biotreatment of PAHs polluted soils.
  • Global NEST Journal 11 (3): 251-259. Miẻge, C., Bouzige, M., Nicol, S., Dugary, J., Pichon, V., Hennion, M. C. (1998) Selective immunoclean-up followed by liquid or gas chromatography for the monitoring of polycyclic aromatic hydrocarbons in urban waste water and sewage sludges used for soil amendment. J. Chromatogr. A 859 (1): 29-39.
  • Muel, B., Lacroix, G. (1960) Analyses des hydrocarbures aromatiques polynucléaires dans l’air. Bulletin de la société chimique de France 11-12: 2139-2147.
  • Nirmaier, H. P., Fisher, E., Meyer, A., Henze, G. (1996) Determination of polycyclic aromatic hydrocarbons in water samples using high performance liquid chromatography with amperometric detection. J. Chromatogr. A 730: 169-1
  • Obana, H., Hori, S., Nakamura, A., Kashimoto, T. (1983) Uptake and release of polynuclear aromatic hydrocarbons by short-necked clams (Tapes japonica). Water Res. 17: 1183-1187.
  • Pino, V., Ayala, J. H., Afonso, A. M., González, V. (2002) Determination of polyaromatic hydrocarbons in seawater by high-performance liquid chromatographywith fluorescence detection following micelle-mediated preconcentration. J. Chromatgr. A 949: 291-299.
  • Rima, J., Lamotte, M., Joussot-Dubien, J. (1982) Application of the shpolskii effect to quantitative analysis of monomethylphenanthrene isomers. Analytical Chemistry 54: 1059-1070.
  • Shariati-Feizabadi, Sh., Yamini, Y., Bahramifar, N. (2003) Determination of some polycyclic aromatic hydrocarbons (PAHs) from aqueous solutions. Anal. Chim. Acta 489 (1): 21-31.
  • Vagi, M. C., Kostopoulou, M. N., Petsas, A. S., Lalousi, M. E., Rasouli, Ch, Lekkas, T. D. (2005) Toxicity of organophoshorous pesticides to the marine alga Tetraselmis suecica. Global NEST Journal 7 (2): 222-227.
  • Varanasi, U., Reichert, W. L., Stein, J. E., Brown, D. W., Sanborn, H. R. (1985)
  • Wise, S. A., Sander, L. C., May, W. E. (1993) Determination of polycyclic aromatic hydrocarbons by liquid chromatography. J. Chromatogr. A 642 (1-2): 329-3
  • Wolska, L., Galer, K., Gỏrecki, T., Namiesnik, J. (1999) Surface water procedure for chromatographic determination for polycyclic aromatic hydrocarbons and polychlorinated biphenyl. Talanta 50 (5): 985-991.
  • Zaghden, H., Kallel, M., Elleuch, B., Oudot, J., Saliot, A. (2007) Sources and distribution of aliphatic and polyaromatic hydrocarbons in sediments of Sfax,
  • Tunisia, Mediterranean Sea. Marine Chemistry 105 (1-2): 70-89.
There are 25 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Jamil Rima This is me

Publication Date April 1, 2013
Published in Issue Year 2013 Volume: 19 Issue: 1

Cite

APA Rima, J. . (2013). HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast. Journal of Black Sea / Mediterranean Environment, 19(1), 58-69.
AMA Rima J. HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast. Journal of Black Sea / Mediterranean Environment. April 2013;19(1):58-69.
Chicago Rima, Jamil. “HPLC and Spectrofluorimetric Determination of Pyrene in the Lebanese Coast”. Journal of Black Sea / Mediterranean Environment 19, no. 1 (April 2013): 58-69.
EndNote Rima J (April 1, 2013) HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast. Journal of Black Sea / Mediterranean Environment 19 1 58–69.
IEEE J. . Rima, “HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast”, Journal of Black Sea / Mediterranean Environment, vol. 19, no. 1, pp. 58–69, 2013.
ISNAD Rima, Jamil. “HPLC and Spectrofluorimetric Determination of Pyrene in the Lebanese Coast”. Journal of Black Sea / Mediterranean Environment 19/1 (April 2013), 58-69.
JAMA Rima J. HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast. Journal of Black Sea / Mediterranean Environment. 2013;19:58–69.
MLA Rima, Jamil. “HPLC and Spectrofluorimetric Determination of Pyrene in the Lebanese Coast”. Journal of Black Sea / Mediterranean Environment, vol. 19, no. 1, 2013, pp. 58-69.
Vancouver Rima J. HPLC and spectrofluorimetric determination of pyrene in the Lebanese coast. Journal of Black Sea / Mediterranean Environment. 2013;19(1):58-69.