In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation

Abstract

Objectives: Ellagic acid is a plant-derived polyphenolic compound. The aim of this study is to investigate in vitro effects of ellagic acid on the proliferation and viability of rat C6 glioma cell lineage depending on dose and time and also is to evaluate ultrastructural changes in C6 glioma spheroids. Materials and methods: Effects of ellagic acid on rat C6 glioma cell line were investigated by using two-dimensional models of tumor cell culture depending on dose and time. The effects of Ellagic acid was evaluated as cell proliferation, cell viability, and synthesis phase of cell cycle, and cell structure at 24th, 48th, 72nd hours. Cell structure was evaluated at 24th and 72nd hours in three dimensional cell culture spheroid models. Results: Reducing effect of ellagic acid on cell proliferation and cell viability was seen in two dimensional cultures (p0. 05). Electron micrographs in which three dimensional cell culture spheroid models was investigated, structural changes was found to be different from the control group. Conclusions: In the evaluation of effects of ellagic acid on rat C6 glioma cells using two-dimensional culture models, it was observed that ellagic acid caused reduced cell viability, deterioration in cell structure and prevented cell proliferation.

Keywords

• Ellagic acid, C6 glioma cells, cell culture, brain tumor, glioma

Elajik asidin sıçan C6 glioma hücre kültürlerinde sitotoksisite ve proliferasyon üzerine in vitro etkileri

Öz

Amaç: Elajik asit bitkilerden elde edilen polifenolik bir bileşiktir. Bu çalışmanın amacı, elajik asidin sıçan C6 glioma hücre dizilerinde proliferasyon ve yaşama yeteneği üzerine in vitro etkilerini doz ve zamana bağlı olarak araştırmak ve C6 glioma küremsi cisimlerinde oluşan elektronmikroskopik değişiklikleri değerlendirmektir. Gereç ve yöntem: Elajik asidin sıçan C6 glioma hücre dizisi üzerine etkileri doz ve zamana bağlı olarak iki boyutlu tümör hücre kültürü kullanılarak araştırıldı. Elajik asidin etkileri için üç boyutlu hücre kültürü küremsi modellerinde; hücre çoğalması, hücre yaşamsallığı, hücre döngüsünün sentez fazı ve hücre yapılarının 24, 48 ve 72. saatlerdeki durumu değerlendirildi. Bulgular: İki boyutlu hücre kültürlerinde hücre yaşama yeteneği ve hücre çoğalması üzerinde elajik asidin baskılayıcı etkisi görüldü (p0.05). Üç boyutlu hücre kültürü küremsi modellerinde, elektronmikroskopik incelemede kontrol grubundan farklı yapısal değişiklikler bulundu. Sonuç: İki boyutlu kültür modelleri kullanıldığında, elajik asidin sıçan C6 glioma hücre kültürleri üzerine olan etkilerinin değerlendirilmesi sonucunda; elajik asidin hücre yaşayabilirliğini azalttığı, hücre yapısında bozulmalara neden olduğu ve hücre çoğalmasını engellediği bulundu.

Anahtar Kelimeler

• Elajik asit, C6 glioma hücreleri, hücre kültürü, beyin tümörü, glioma

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