The effects of propolis on cytokine production in lipopolysaccharide-stimulated macrophages

Abstract

Objectives: Propolis, a bee-product, has attracted researchers\' interest in recent years because of several biological and pharmacological properties. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria and has an important role in the pathogenesis of septic shock and several inflammatory diseases by causing excessive release of inflammatory cytokines. The aim of this study was to investigate the effects of ethanol extract of propolis collected in Kayseri and its surroundings on production of pro-inflammatory cytokines in LPS-stimulated macrophages. Materials and methods: In vitro, U937 human macrophage cells were grown in RPMI-1640 medium supplemented with fetal bovine serum (10%) and penicillin-streptomycin (2%) and divided into: control, LPS treated, and propolis+LPS treated cell groups. After incubation in an atmosphere of 5% CO2 and at 37°C of cells, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α levels were measured in cell-free supernatants by ELISA. Results: IL-1β, IL-6 and TNF-α levels increased in LPS treated cell group according to control, statistically significant. Each cytokine levels significantly decreased in LPS and propolis treated cell group according to only LPS treated cell group (p

Keywords

• Lipopolysaccharide, macrophage, pro-inflammatory cytokine, propolis

The effects of propolis on cytokine production in lipopolysaccharide-stimulated macrophages

Öz

Amaç: Propolis, çeşitli biyolojik ve farmakolojik özelliklerinden dolayı son yıllarda araştırmacıların ilgisini çeken bir arı ürünüdür. Lipopolisakkarit (LPS), Gram negatif bakterilerin dış membran komponenti olup, inflamatuar sitokinlerin aşırı üretimine yol açarak septik şok ve çeşitli inflamatuar hastalıkların patogenezinde önemli rol oynar. Bu çalışmada Kayseri ve çevresinden toplanan propolis\'in etanolik ekstraktının, LPS ile uyarılmış makrofajlarda pro-inflamatuar sitokinlerin üretimi üzerine etkisinin araştırılması amaçlanmıştır. Gereç ve yöntem: İn vitro olarak, fetal bovin serum (%10) ve penisilin-streptomisin (%2) ilaveli RPMI-1640 besiyerinde üretilen U937 insan makrofaj hücreleri, kontrol, LPS uygulanan ve propolis+LPS uygulanan hücreler olmak üzere üç gruba ayrıldı. Hücrelerin 37°C ve %5 CO2\'li atmosferde inkübasyonu sonunda, hücre süpernatantlarında interlökin (IL)-1β, IL-6 ve tümör nekrozis faktör (TNF)-α düzeyleri ELISA ile ölçüldü. Bulgular: LPS uygulanan hücre grubunda, IL-1β, IL-6 ve TNF-α düzeylerinde kontrole göre istatistiksel olarak anlamlı bir artış saptanırken, LPS ve propolis uygulanan hücre grubunda -yalnız LPS uygulanan hücre grubuna göre- her üç sitokin düzeyinde de anlamlı bir azalma olduğu tespit edildi (p

Anahtar Kelimeler

• Lipopolisakkarit, makrofaj, pro-inflamatuar sitokin, propolis

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