Serum paraoxanase-1 activity in patients with acute otitis media

Öz

Amaç: Paraoksonaz (PON) yüksek dansiteli lipoprotein (HDL) ile ilişkili antioksidan bir enzimdir. Bu çalışmada akut otitis media (AOM) hastaları ile sağlıklı kontrol grubu arasında PON 1 aktivitesini karşılaştırmak amaçlanmaktadır. Gereç ve yöntem: AOM hastalarında prospektif, kontrollü bir çalışma yaptık. Çalışmaya 30 AOM hastası (17 erkek; yaş, 15 ± 9 yıl) ve 30 kontrol grubu (16 erkek; yaş, 16 ± 5) olmak üzere 60 birey dahil edildi. Hasta ve kontrol grubunda Mackness\'in metodu ile PON 1 aktivitesi ölçüldü. Bulgular: Akut otitis media hasta grubunda, kontrol grubuna göre PON 1 aktivitesi düşük düzeyde idi (155 ± 95 U/L ve 210 ± 96 U/L, P=0.04). Sonuç: Akut otitis media\'da oksidatif stres oluşmaktadır. Oluşan oksidatif hasarı önlemek amacıyla PON 1 enzimi daha fazla tüketilmektedir. Bu nedenle AOM hastalarında bu enzim aktivitesi düşük düzeyde elde edilmektedir.

Anahtar Kelimeler

• Akut otitis media, paraoksanaz 1 aktivitesi, oksidatif stres

Serum paraoxanase-1 activity in patients with acute otitis media

Abstract

Objectives: Paraoxonase is a high-density lipoprotein (HDL)-associated antioxidant enzyme. We aimed to compare the activity of serum paraoxanase-1 (PON 1) in healthy controls and patients with acute otitis media (AOM). Subjects and methods: We performed a prospective controlled trial in patients with AOM. A total of 60 subjects, 30 patients with AOM (17 male; age, 15 ± 9 years) and 30 age- and sex-matched controls (16 male; age, 16 ± 5 years) were enrolled in this study. PON 1 activity was measured using Mackness\' methods. Results: PON 1 activity was significantly lower in the AOM group compared to control group (155 ± 95 U/L vs. 210 ± 96 U/L, P=0.04). Conclusions: The present study demostrates that serum PON 1 activity is low in AOM, similar to other conditions with increased oxidative stress. We speculate that PON 1 enzyme is consumed at a greater rate during AOM in order to prevent increased oxidative damage. J Clin Exp Invest 2011; 2 (4): 412-415

Keywords

• Acute otitis media, paraoxanase-1 activity, oxidative strees

References

1. Pukander J, Sipila M, Kataja M, Karma P. Estimating the risk of acute otitis media among urban children. Ann Otol Rhinol Laryngol 1990;149(3):18-20.
2. Serafini M, Del Rio D. Understanding the association between dietary antioxidants, redox status, and dis- ease: is the total antioxidant capacity the right tool? Redox Report 2004;9(3):145-52.
3. Seidman MD, Ahmad N, Joshi D, Seidman J, Thawani S, Quirk WS. Age-related hearing loss and its associ- ation with reactive oxygen species and mitochondrial DNA damage. Acta Otolaryngol Suppl 2004;(552):16- 24.
4. Doğru H, Delibaş N, Döner F, Tuz M, Uygur K. Free radical damage in nasal polyp tissue. Otolaryngol Head Neck Surg 2001;124(5):570-2.
5. Parks RR, Huang CC, Haddad J Jr. Evidence of oxy- gen radical injury in experimental otitis media. Laryn- goscope 1994;104(11):1389-92.
6. Rasheed MH, Beevi SS, Geetha A. Enhanced lipid per- oxidation and nitric oxide products with deranged anti- oxidant status in patients with head and neck squamous cell carcinoma. Oral Oncol 2007;43(4):333-8.
7. Tissié G, Guillermet V, Latour E, Coquelet C, Bonne C. Oxidative stress and lens opacity: an overall approach to screening anticataractous drugs. Ophthalmic Res 1988;20(1):27-30.
8. Diaz MN, Frei B, Vita JA, Keaney JF. Antioxidants and atherosclerotic heart disease. N Engl J Med 1997;337(6):408-16.

9. Wickens D, Wilkins MH, Lunec J, Ball G, Dormandy TL. Free radical oxidation (peroxidation) products in se- rum and synovial fluid in rheumatoid arthritis. J Rheu- matol 1981;8(2):233-45.
10. Machlin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrients. FASEB J 1987;1(6):441-5.
11. Lipincott W. Paraoxonase a cadioprotective en- zyme: continuing issuess. Curr Opin Lipidol 2004;15(3):261-7.
12. Mackness MI, Arrol S, Durrington PN. Paraoxonase prevents accumulation of lipoperoxides in low-density lipoprotein. FEBS Lett 1991;286(1-2):152-4.
13. Haliwell B. Reactive oxygen species in living system: Source biochemistry, and role in human disease. Am J Med 1991(3);91:149-235.
14. Takoudes TG, Haddad J Jr. Lipid peroxides in middle ear fluid after acute otitis media in guinea pigs. Otol Rhinol Laryngol 1999;108(6):564-8.
15. Bodolay E, Seres I, Szodoray P, et al. Evaluation of paraoxonase activity in patients with mixed connec- tive tissue disease. J Rheumatol 2008;35(2):237-43.
16. Selek S, Cosar N, Kocyigit A, et al. PON1 activity and total oxidant status in patients with active pulmonary tuberculosis. Clin Biochem 2008;41(3):140-4.
17. Isik A, Koca SS, Ustundag B, Selek S. Decreased total antioxidant response and increased oxida- tive stress in Behcet’s disease. Tohoku J Exp Med 2007;212(2):133-41.
18. Aslan M, Kosecik M, Horoz M, Selek S, Celik H, Erel O. Assessment of paraoxonase and arylesterase ac- tivities in patients with iron deficiency anemia. Athero- sclerosis 2007; 191(2): 397-402.
19. Emin T, Elkiran N. Serum paraoxonase and ary- lesterase activities in patients with lung cancer in a Turkish population. BMC Cancer 2007;7:48-52.