Nanoparticles (nanobacteria) responsible from calcification

Year 2011, , 463 - 467, 01.12.2011

Tuba Dal Mehmet Sinan Dal

https://doi.org/10.5799/ahinjs.01.2011.04.0098

Abstract

Calcifying nanoparticles (CNPs) are particles smaller (80-500nm) than known bacteria and have bacteria-like features (membrane structures, in two division, colony formation). CNPs have shown in human and animal sera, human\'s pathological calcifications (kidney stones, dental pulp stones, heart valve calcifications, arterial calcifications, psammoma bodies in ovarian cancer, etc.), the nature\'s calcifications (travertines, etc.) and Mars meteors. CNPs are able to grow in Dulbecco's modified eagle medium (DMEM), can be examined by transmission and scanning electron microscopes (TEM, SEM). Previously CNPs were known as small bacteria (nanobacteria) that cause calcifications by precipitating calcium and phosphate crystals into the capsules. In recent years, the opinion that CNPs are complex structures consisting of protein and minerals has become more important. According to this opinion some proteins (such as albumin, fetuin) known as the physiological inhibitor of calcification cause to work CNPs as a nidus for calcification and the formation of hydroxy apatite crystals. Despite the known, unknowns are still the majority about CNPs. We also aimed to emphasize with this article, the need for new studies about CNPs that are thought to have the roles in pathological calcifications. J Clin Exp Invest 2011; 2 (4): 463-467

Keywords

Nanoparticle, pathological calcification, nanobacteria

Kalsifikasyondan sorumlu nanopartiküller (nanobakteriler)

Abstract

Kalsifikasyondan sorumlu nanopartiküller (KNP) bilinen bakterilerden daha küçük (80-500 nm), bakterilere benzer özellikler (membran yapıları, ikiye bölünme, koloni oluşumu) gösteren partiküllerdir. KNP\'ler insan ve hayvan serumlarında, insanlarda görülen patolojik kalsifikasyonlarda (böbrek taşları, dental pulpa taşları, kalp kapağı kalsifikasyonları, arter kalsifikasyonları, over kanserinde görülen psammoma cisimcikleri vs.), doğadaki kalsifikasyonlarda (travertenler vs.), Mars meteorlarında gösterilmişlerdir. KNP'ler Dulbecco\' nun modifiye eagle besiyeri (DMEM) ile yapılan hücre kültürlerinde üreme özelliği göstermekte transmisyon ve scanning elektron mikroskoplar (TEM, SEM) ile incelenebilmektedir. Önceleri KNP\'ler kalsiyum fostat kristallerini kapsüllerine çökelterek kalsifikasyonlara neden olan küçük bakteriler (nanobakteriler) olarak biliniyordu. Son yıllarda KNP\'lerin mineral ve proteinden oluşan kompleks yapılar oldukları görüşü önem kazanmıştır. Bu görüşe göre fizyolojik koşullarda kalsifikasyon inhibitörü olarak bilinen albumin, fetuin gibi proteinler; ortamda aşırı derecede kalsiyum, fosfor varlığında KNP\'lerin kalsifikasyon nidusu olarak işlev görmesine ve hidroksi apatit kristallerinin oluşmasına neden olmaktadır. KNP\'lerle ilgili bilinenlere rağmen bilinmeyenler halen çoğunluktadır. Biz de bu makalemiz ile patolojik kalsifikasyonlarda rolleri olduğu düşünülen KNP\'lerle ilgili yeni çalışmaların gerekliliğini vurgulamayı amaçladık.

Keywords

Nanopartikül, patolojik kalsifikasyon, nanobakteriler

References

• Kajander EO, Kuronen I, Akerman K, Pelttari A, Ciftcio- glu N. Nanobacteria from blood, the smallest cultur- able autonomously replicating agent on Earth. Proc SPIE Int Soc Opt Eng 1997;3111(3):420-8.
• Ciftcioglu N, Bjorklund M, Kajander EO. Stone forma- tion and calcification by nanobacteria in human body. Proc SPIE Int Soc Opt Eng 1998;3441(1):105-11.
• Ciftcioglu N, Bjorklund M, Kuorikoski K, Bergstrom K, Kajander EO. Nanobaceria: an infectious cause for kid- ney stone formation. Kidney Int 1999;56(5):1893-8.
• Folk, Robert L. Nanobacteria: surely not figments, but what under heaven are they? Natural Science 1997;1(3):1-4.
• Romanek CS, Thomas KL, Gibson EK Jr, McKay DS, Socki RA. Petrogenesis of carbon-and sulfur-bearing minerals in the martian meteorite ALH 84001. Lunar Planet Sci; XXVI 1995; 26(12):1183-4.
• McKay DS, Gibson EK, Thomas-Keprta KL, Vali H, Romanek CS. No ‘nanofossils in martian meteorite: reply. Nature 2007; 390(2):455-6.
• Kajander EO, Ciftcioglu N, Miller-Hjelle MA, Hjelle JT. Nanobacteria: controversial pathogens in nephro- lithiasis and polycystic kidney disease. Cur Opinions Nephrol Hypertension 2001;10 (3):445-52.
• Sedivy R, Battistutti WB. Nanobacteria promote crys- tallization of psammoma bodies in ovarian cancer. APMIS 2003;111(8):951-4.
• Wen Y, Li YG, Yang ZL et al. Detection of nanobacteria in serum, bile and gallbladder mucosa of patients with cholecystolithiasis. Chin Med J 2005;118(5):421-4.
• Drancourt M, Jacomo V, Lépidi H et al. Attempted isola- tion of nanobacterium sp. microorganisms from upper urinary tract stones. J Clin Microbiol 2003;41(1):368- 72.
• Puskas LG, Tiszlavicz L, Razga Z, Torday LL, Krenacs T, Papp JG. Detection of nanobacteria-like particles in human atherosclerotic plaques. Acta Biol Hung 2005;56 (3-4):233-45.
• Freeman RV, Otto CM. Spectrum of calcific aortic valve disease: pathogenesis, disease progression, and treatment strategies. Circulation 2005;111(24):3316- 26.
• Piper C, Dreier J, Kleesiek K, Körfer R, Horstkotte D. Nanobacteria to promote degenerative aortic valve stenosis? J Heart Valve Dis 2006;15(4):499-501.
• Bratos-Pérez MA, Sánchez PL, García de Cruz S et al. Association between self-replicating calcifying nanoparticles and aortic stenosis: a possible link to valve calcification. Eur Heart J 2008;29(3):371-6.
• Hu YR, Zhao Y, Sun YW, et al. Detection of nano- bacteria-like material from calcified cardiac valves with rheumatic heart disease. Cardiovasc Pathol 2009;19(5):286-92.
• Candemir B, Ertas FS, Cansin Tulunay Kaya, Ozdol C, Hasan T, Akan OA, Sahin M, Erol C. Association be- tween antibodies against calcifying nanoparticles and mitral annular calcification. J Heart Valve 2010;19(6): 745-52.
• Tsurumoto T, Matsumoto T, Yonekura A, Shindo H. Nanobacteria-like particles in human arthritic synovial fluids. J Proteome Res 2006;5(5):1276-8.
• Altundag K, Altundag O, Akyurek S, et al. Pos- sible association between nanobacteria and malig- nant microcalcifications in breast cancer. Breast J 2006;12(3):287-9.
• Agababov RM, Abashina TN, Suzina NE et al. Link between the early calciumdeposition in placenta and nanobacterial-like infection. J Biosci 2007;32 (6):1163-8.
• Zhang Q-H, Lu G-S, Shen X-C, Zhou Z-S, Fang Q, Zhang X, Li L-K, Jin X-Y, Song B. Nanobacteria May Be Linked to Testicular Microlithiasis in Infertility. J An- drol 2010;31(2): 121-5.
• Zeng JF, Zhang W, Jiang HW, Ling JQ. Isolation, cul- tivation and initial identification of Nanobacteria from dental pulp stone. Zhonghua Kou Qiang Yi Xue Za Zhi 2006;41(8):498-501.
• Zhang SM, Tian F, Jiang XQ et al. Evidence for calcifying nanoparticles in gingival crevicular fluid and dental calculus in periodontitis. J Periodontol 2009;80(9):1462-70.
• Zhou Z, Hong L, Shen X et al. Detection of nano- bacteria infection in type III prostatitis. Urology 2008;71(6):1091-5.
• Zhang QH, Shen XC, Zhou ZS, et al: Decreased nano- bacteria levels and symptoms of nanobacteria-associ- ated interstitial cystitis/painful bladder syndrome after tetracycline treatment. Int Urogynecol J Pelvic Floor Dysfunct 2010;21(1):103-9.
• Cisar JO, XU DQ, Thompson J, Swaim W, Hu L, and Kopecko DJ. An alternative interpretation of nanobac- teria-inducted biomineralization. Proc Natl Acad Sci USA 2000;97(21):1511-5.
• Raoult D, Drancourt M, Azza S et al. Nanobacte- ria are mineralo fetuin complexes. PLoS Pathog 2008;4(2):41-5.
• Martel J, Young J D-E. Purported nanobacteria in hu- man blood as calcium carbonate nanoparticles. PNAS 2008;105(14): 5549-54.
• Young DJ, Martel J, Young L, Wu C-Y, Young A, Young D. Putative nanobacteria Represent physiological remnants and culture by-products of normal calcim homeostasis. Plos One 2009;4(2):4417-20.
• Yang F, Zeng J, Zhang W, Sun X, Ling J. Evaluation of the interaction between calcifying nanoparticles and human dental pulp cells: a preliminary investigation. Int J Nanomedicine 2011;15(6):13-8.
• Lin Y, Zheng R, He H, Du H. Application of biomimet- ic mineralization: a prophylactic therapy for cracked teeth? Med Hypotheses 2009;73(4):493-4.