The effect of extremely low frequency magnetic field on heart tissue iron density

Year 2011, , 144 - 148, 01.06.2011

Sevda Söker Cemil Sert Mustafa Deniz Ercan Ayaz Selçuk Tunik Yusuf Nergiz

https://doi.org/10.5799/ahinjs.01.2011.02.0228

Abstract

Objectives: The aim of this histological study was to investigate the effects of extremely low frequency, low intensity magnetic field on the heart muscle ıron density. Materials and methods: In this study, 45 male Spraque Dawley rats were introduced and were divided into three groups as sham, control and experiment group. The experimental group was exposed to a 0.25 mT to Extremely Low Frequency Magnetic Field (ELF-MF) for 14 days, 3h a day in metacrylate boxes. The sham group was treated like the experimental group, except for ELF-MF exposure. For control, nothing applied to rats in this group and they completed their life cycle in the cage during the study period. After exposure period, the rats were sacrificed under ketalar an-esthesia (50 mg / kg, intramuscularly). Heart tissues were immediately fixed in 10% neutral formaldehyde and embed-ded in paraffin blocks. Histological sections from cardiac tissue stained by hematoxylin-eosin, Perls' Prussian blue for iron pigments. Histological slides were photographed under a Nikon DS-2MV photomicroscope. Results: The architecture and histology of the control, sham and experimental group were observed as normal. No differences were observed between the control, sham and experimental rat groups in the iron stain of heart tissues. Conclusion: As a result of our study, we did not observe differences between the control and ELF-MF (experimental) group. In this investigation we demonstrated that the exposure of cardiac tissue of rats to the ELF-MF did not change in the iron stain study.

Keywords

Extremely low frequency magnetic field, heart, iron.

Aşırı düşük frekanslı manyetik alanın kalp dokusu demir yoğunluğu üzerine etkisi

Abstract

Amaç: Bu histolojik çalışmanın amacı; aşırı düşük frekanslı, düşük yoğunluklu manyetik alanın kalp dokusu demir yo-ğunluğu üzerindeki etkilerini araştırmaktır. Gereç ve yöntem: Bu çalışmada; sham, kontrol ve deney grubu olarak üçe bölünen 45 Spraque Dawley erkek rat kul-lanıldı. Deney grubu günde üç saat 14 gün boyunca metakrilat kutularda 0.25 mT oldukça düşük frekanslı manyetik alana (ELF-MF) maruz bırakıldı. Sham grubu, ELF-MF uygulaması dışında deney grubuna benzer şekilde uygulamaya maruz bırakıldı. Kontrol grubu ratlara hiçbir şey uygulanmadı ve fareler çalışma peryodu boyunca yaşam sikluslarını kafes içinde tamamladı. Yapılan işlem sonrasında, 50 mg/kg intramuskuler ketalar anestezisi uygulanarak ratlar sakrifiye edildi Kalp dokuları hemen %10 nötral formalinde fikse edilerek parafin bloklara gömüldü. Kalp dokusunun histolojik örnekleri Hematoksilen-Eosin ve demir pigmentleri için Perls' Prussian blue boyası ile boyandı. Nikon DS-2MV fo-tomikroskopta histolojik preparatlar fotoğraflandı. Bulgular: Kontrol, sham ve deney grubumuzun histolojik yapısı normal gözlendi. Kontrol, sham ve deney grubu arasında kalp dokusu demir boyaması açısından farklılık gözlenmedi. Sonuç: Çalışmamız sonucunda, kontrol ve ELF-MF (deney) grubu arasında farklılık saptamadık. Bu çalışmada, ELF-MF'ye maruz kalan farelerin kalp dokusunda demir boyaması ile değişiklik olmadığı saptandı.

Keywords

Aşırı düşük frekanslı elektromanyetik alan, kalp, demir.

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