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Yardımcı üreme tekniklerinde endometriyal kokültür hücreleri

Year 2015, , 357 - 363, 03.01.2016
https://doi.org/10.5799/ahinjs.01.2015.04.0549

Abstract

Amaç: Endometriyum, üreme biyolojisinde çok önemli bir
rol oynar ve ona bağlı olarak gelişen patolojilerden biri
implantasyonda yaşanan olumsuzluklardır. İmplantasyon
başarısızlıkları, kadın infertilitesi için önemli süreçlerdendir
ve çözümüne dair geliştirilen yöntemlerden biri ise
endometriyal kokültür uygulamalarıdır. Bu çalışmada, in
vitro monolayer endometriyal kokültürdeki hücre tiplerinin
ayırt edilmesi, doku dinamiğinin aydınlatılabilmesi ve klinik
uygulamalarda kullanımı açısından yararlılığının gösterilmesi
amaçlandı.
Yöntemler: Çalışmamıza 8 fertil ve Tekrarlayan İmplantasyon
Başarısızlığı (TİB) yaşayan 16 infertil kadından
oluşan 2 grup dâhil edildi. Negatif basınçlı pipelle aspirasyon
yapılarak endometriyal doku örnekleri elde edildi.
Embriyoyu blastosist aşamasına götüreceği düşünülen
“Endometriyal Epitel-Gland hücresi (EE)” ve “Stromal
(ES) hücreler” ayrıştırılıp kültürleri yapıldı. Daha sonra
bu dokular ışık mikroskobik doku takibine alındı. Kokültür
hücreleri, toluidin mavisi ile boyandıktan sonra gruplandırıldı.
Hücre tiplerine ait sayısal değerler ile çalışmanın
istatiksel analizi ki-kare metodu kullanılarak yapıldı.
Bulgular: Kokültürlerde hem fertil hem de TİB grubundan
elde edilen hücre tipleri ışık mikroskobik incelemeyle
morfolojik özelliklerine göre ayırt edildi. Her iki grupta da,
vakuollü, mikrovilluslu ve sitoplazmik uzantılı olarak isimlendirilen
hücreler tanımlandı.
Sonuç: Her iki grubun, endometriyum dokularından elde
edilen kokültürleri ışık mikroskobik düzeyde incelendi.
Böylece gruplar arası farklılıkların belirlenmesi kokültürdeki
hücre yapılarının sınıflandırılması sağlandı ve endometriyal
kokültür uygulamalarının embriyonun gelişiminde
faydalı olabileceği kanısına varıldı.

References

  • Maugey-Laulom B, Commenges-Ducos M, Jullien V, et al. Endometrial vascularity and on going pregnanacy after IVF. Eur J Obstet Gynecol Reprod Bio 2002;104:137-143.
  • Bahar L, Baykal T. Endometriyal reseptivitenin implantasyondaki
  • rolü. Mersin Univ Saglık Bilim Derg 2008;1:1-6.
  • Polanski LT, Baumgarten MN, Quenby S, et al. What exactly do we mean by ‘recurrent implantation failure’? A systematic review and opinion. Reprod Biomed Online 2014;28:409-423.
  • Singh M, Chaudhry P, Asselin E. Bridging endometrial receptivity and implantation: network of hormones, cytokines, and growth factors. J Endocrinol 2011;210:5-14.
  • Bee K, Tan BK, Vandekerckhove P, et al. Investigation and current management of recurrent IVF treatment failure in the UK. Int J O&G 2005;112:773-780.
  • Coughlan C, Ledger W, Wang Q, et al. Recurrent implantation failure: definition and management. Reprod Biomed Online 2014;28:14-38.
  • Achache H, Revel A. Endometril receptivity markers, the journey to successful embryo implantation. Hum Reprod Update 2006;12:731-746.
  • Racicot KE, Wu¨nsche V, Auerbach B, et al. Human chorionic gonadotropin enhances trophoblast–epithelial ınteraction in an in vitro model of human implantation. Reprod Sci 2014;21:1274-1280.
  • Nardo LG. Human embriyo implantation failure and recurrent miscarriage: basic science and clinical. Reprod Biomed Online 2006;13:11-2.
  • Plachot M, Alvarez S, Merviel P, et al. Role of endometrial cells in “in vitro” embryo development. Assist Reprod Rev 1994;4:85–95.
  • Bahar L, Eras N. Endometrial ko-kültür uygulamaları. Genel Tıp Derg 2012;22:31-36.
  • Kim YB, Ahn SH, Chang DY, et al. Vero cell co-culture counteracts the detrimental effects of hydrosalpinx fluid on the development of mouse embryos in vitro. J Korean Med Sci 2002;17:217-219.
  • Nottola SA, Heyn R, Camboni A, et al. Ultrastructural characteristics of human granulosa cells in a coculture system for in vitro fertilization. Microsc Res Tech 2006;69:508-516.
  • Bongso A, Fong, CY, Ng SC. Human embryonic behavior in a sequencial human oviduct-endometrial coculture system. Fertil Steril 1994;61:976–978.
  • Barmat LI, Liu H-C, Spandorfer CD, et al. Human preembryo development on autologous endometrial coculture versus conventional medium: A randomized trial. Fertil Steril 1998;70:1109-1113.
  • Barmat LI, Liu H-C, Spandorfer SD, et al. Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. J Assist Reprod Genet 1999;16:121-127.
  • Seferli R, Tokmak A, Ersoy AÖ, et al. Assessment of reproductive results of infertile patients who had undergone operative hysteroscopy for intrauterine pathology. J Clin Exp Invest 2015;6:52-58.
  • Ledee-Bataille N, Lapree-Delage G, Taupin JL. Concentration
  • of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation. Hum Reprod 2002;17:213-218.
  • Cavalcante MB, Costa FD, Barini R, Júnior EA. Granulocyte
  • colony-stimulating factor and reproductive medicine: A review. Iran J Reprod Med 2015;13:195-202.
  • Tada Y, Kitaya K, Amano N, et al. A pilot survey on obstetric complications in pregnant women with a history of repeated embryo implantation failure and those undergoing single local endometrial injury. Clin Exp Obstet Gynecol 2015;42:176-178.
  • Potdar N, Gelbaya T, Nardo LG. Endometrial injury to overcome recurrent embryo implantation failure: a systematic review and meta-analysis. Reprod Biomed Online 2012;25:561-571.
  • Zhou L, Li R, Wang R, et al. Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates. Fertil Steril 2008;89:1166-1176.
  • Zhang XH, Liu ZZ, Tang MX, et al. Morphological changes and expression of cytokine after local endometrial injury in a mouse model. Reprod Sci 2015; doi:10.1177/1933719115580999.
  • Melnick AP, Murphy EM, Masbou AK, et al. Autologous endometrial coculture biopsy: is timing everything? Fertil Steril 2015;104:104-109.
  • Simón C, Mercader A, Garcia-Velasco J, et al. Coculture of human embryos with autologous human endometrial human endometrial epithelial cells in patients with implantation failure. J Clin Endocrinol Metab 1999;84:2638-2646.
  • Urman B, Yakın K, Balaban B. Recurrent implantation failure in assisted reproduction: how to counsel and manage. General onsiderations and treatment options that may benefit the couple. RBM Online 2005;11:371-381.
  • Spandorfer SD, Barmat LI, Liu H-C, et al. Granulocyte Macrophage-Colony Stimulating Factor production by autologous endometrial co-culture is associated with outcome for IVF patients with a history of multiple implantation failures. Am J Reprod Immunol 1998;40:377–381.
  • Spandorfer SD, Soslow R, Clark R, et al. Histologic characteristics of the endometrium predicts success when utilizing autologous endometrial coculture in patients with IVF failure. J Assist Reprod Genet 2006;23:185-189.
  • Strowitzki T, Germeyer A, Popovici R, Wolff M. The human endometrium as a fertility-determining factor.Hum Reprod Update 2006;12:617-630.
  • Dimitriadis E, White CA, Jones RL, Salamonsen LA. Cytokines, chemokines and growth factors in endometrium related to implantation Hum Reprod Update 2005;11:613-630.
  • İskender B, İzgi K, Şanlıoğlu S, Canatan H. Human embryonic stem cells and microenvironment. J Clin Exp Invest 2014;5:486-495.
Year 2015, , 357 - 363, 03.01.2016
https://doi.org/10.5799/ahinjs.01.2015.04.0549

Abstract

References

  • Maugey-Laulom B, Commenges-Ducos M, Jullien V, et al. Endometrial vascularity and on going pregnanacy after IVF. Eur J Obstet Gynecol Reprod Bio 2002;104:137-143.
  • Bahar L, Baykal T. Endometriyal reseptivitenin implantasyondaki
  • rolü. Mersin Univ Saglık Bilim Derg 2008;1:1-6.
  • Polanski LT, Baumgarten MN, Quenby S, et al. What exactly do we mean by ‘recurrent implantation failure’? A systematic review and opinion. Reprod Biomed Online 2014;28:409-423.
  • Singh M, Chaudhry P, Asselin E. Bridging endometrial receptivity and implantation: network of hormones, cytokines, and growth factors. J Endocrinol 2011;210:5-14.
  • Bee K, Tan BK, Vandekerckhove P, et al. Investigation and current management of recurrent IVF treatment failure in the UK. Int J O&G 2005;112:773-780.
  • Coughlan C, Ledger W, Wang Q, et al. Recurrent implantation failure: definition and management. Reprod Biomed Online 2014;28:14-38.
  • Achache H, Revel A. Endometril receptivity markers, the journey to successful embryo implantation. Hum Reprod Update 2006;12:731-746.
  • Racicot KE, Wu¨nsche V, Auerbach B, et al. Human chorionic gonadotropin enhances trophoblast–epithelial ınteraction in an in vitro model of human implantation. Reprod Sci 2014;21:1274-1280.
  • Nardo LG. Human embriyo implantation failure and recurrent miscarriage: basic science and clinical. Reprod Biomed Online 2006;13:11-2.
  • Plachot M, Alvarez S, Merviel P, et al. Role of endometrial cells in “in vitro” embryo development. Assist Reprod Rev 1994;4:85–95.
  • Bahar L, Eras N. Endometrial ko-kültür uygulamaları. Genel Tıp Derg 2012;22:31-36.
  • Kim YB, Ahn SH, Chang DY, et al. Vero cell co-culture counteracts the detrimental effects of hydrosalpinx fluid on the development of mouse embryos in vitro. J Korean Med Sci 2002;17:217-219.
  • Nottola SA, Heyn R, Camboni A, et al. Ultrastructural characteristics of human granulosa cells in a coculture system for in vitro fertilization. Microsc Res Tech 2006;69:508-516.
  • Bongso A, Fong, CY, Ng SC. Human embryonic behavior in a sequencial human oviduct-endometrial coculture system. Fertil Steril 1994;61:976–978.
  • Barmat LI, Liu H-C, Spandorfer CD, et al. Human preembryo development on autologous endometrial coculture versus conventional medium: A randomized trial. Fertil Steril 1998;70:1109-1113.
  • Barmat LI, Liu H-C, Spandorfer SD, et al. Autologous endometrial co-culture in patients with repeated failures of implantation after in vitro fertilization-embryo transfer. J Assist Reprod Genet 1999;16:121-127.
  • Seferli R, Tokmak A, Ersoy AÖ, et al. Assessment of reproductive results of infertile patients who had undergone operative hysteroscopy for intrauterine pathology. J Clin Exp Invest 2015;6:52-58.
  • Ledee-Bataille N, Lapree-Delage G, Taupin JL. Concentration
  • of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation. Hum Reprod 2002;17:213-218.
  • Cavalcante MB, Costa FD, Barini R, Júnior EA. Granulocyte
  • colony-stimulating factor and reproductive medicine: A review. Iran J Reprod Med 2015;13:195-202.
  • Tada Y, Kitaya K, Amano N, et al. A pilot survey on obstetric complications in pregnant women with a history of repeated embryo implantation failure and those undergoing single local endometrial injury. Clin Exp Obstet Gynecol 2015;42:176-178.
  • Potdar N, Gelbaya T, Nardo LG. Endometrial injury to overcome recurrent embryo implantation failure: a systematic review and meta-analysis. Reprod Biomed Online 2012;25:561-571.
  • Zhou L, Li R, Wang R, et al. Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates. Fertil Steril 2008;89:1166-1176.
  • Zhang XH, Liu ZZ, Tang MX, et al. Morphological changes and expression of cytokine after local endometrial injury in a mouse model. Reprod Sci 2015; doi:10.1177/1933719115580999.
  • Melnick AP, Murphy EM, Masbou AK, et al. Autologous endometrial coculture biopsy: is timing everything? Fertil Steril 2015;104:104-109.
  • Simón C, Mercader A, Garcia-Velasco J, et al. Coculture of human embryos with autologous human endometrial human endometrial epithelial cells in patients with implantation failure. J Clin Endocrinol Metab 1999;84:2638-2646.
  • Urman B, Yakın K, Balaban B. Recurrent implantation failure in assisted reproduction: how to counsel and manage. General onsiderations and treatment options that may benefit the couple. RBM Online 2005;11:371-381.
  • Spandorfer SD, Barmat LI, Liu H-C, et al. Granulocyte Macrophage-Colony Stimulating Factor production by autologous endometrial co-culture is associated with outcome for IVF patients with a history of multiple implantation failures. Am J Reprod Immunol 1998;40:377–381.
  • Spandorfer SD, Soslow R, Clark R, et al. Histologic characteristics of the endometrium predicts success when utilizing autologous endometrial coculture in patients with IVF failure. J Assist Reprod Genet 2006;23:185-189.
  • Strowitzki T, Germeyer A, Popovici R, Wolff M. The human endometrium as a fertility-determining factor.Hum Reprod Update 2006;12:617-630.
  • Dimitriadis E, White CA, Jones RL, Salamonsen LA. Cytokines, chemokines and growth factors in endometrium related to implantation Hum Reprod Update 2005;11:613-630.
  • İskender B, İzgi K, Şanlıoğlu S, Canatan H. Human embryonic stem cells and microenvironment. J Clin Exp Invest 2014;5:486-495.
There are 34 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Leyla Bahar

Semra Kahraman This is me

Publication Date January 3, 2016
Published in Issue Year 2015

Cite

APA Bahar, L., & Kahraman, S. (2016). Yardımcı üreme tekniklerinde endometriyal kokültür hücreleri. Journal of Clinical and Experimental Investigations, 6(4), 357-363. https://doi.org/10.5799/ahinjs.01.2015.04.0549
AMA Bahar L, Kahraman S. Yardımcı üreme tekniklerinde endometriyal kokültür hücreleri. J Clin Exp Invest. January 2016;6(4):357-363. doi:10.5799/ahinjs.01.2015.04.0549
Chicago Bahar, Leyla, and Semra Kahraman. “Yardımcı üreme Tekniklerinde Endometriyal kokültür hücreleri”. Journal of Clinical and Experimental Investigations 6, no. 4 (January 2016): 357-63. https://doi.org/10.5799/ahinjs.01.2015.04.0549.
EndNote Bahar L, Kahraman S (January 1, 2016) Yardımcı üreme tekniklerinde endometriyal kokültür hücreleri. Journal of Clinical and Experimental Investigations 6 4 357–363.
IEEE L. Bahar and S. Kahraman, “Yardımcı üreme tekniklerinde endometriyal kokültür hücreleri”, J Clin Exp Invest, vol. 6, no. 4, pp. 357–363, 2016, doi: 10.5799/ahinjs.01.2015.04.0549.
ISNAD Bahar, Leyla - Kahraman, Semra. “Yardımcı üreme Tekniklerinde Endometriyal kokültür hücreleri”. Journal of Clinical and Experimental Investigations 6/4 (January 2016), 357-363. https://doi.org/10.5799/ahinjs.01.2015.04.0549.
JAMA Bahar L, Kahraman S. Yardımcı üreme tekniklerinde endometriyal kokültür hücreleri. J Clin Exp Invest. 2016;6:357–363.
MLA Bahar, Leyla and Semra Kahraman. “Yardımcı üreme Tekniklerinde Endometriyal kokültür hücreleri”. Journal of Clinical and Experimental Investigations, vol. 6, no. 4, 2016, pp. 357-63, doi:10.5799/ahinjs.01.2015.04.0549.
Vancouver Bahar L, Kahraman S. Yardımcı üreme tekniklerinde endometriyal kokültür hücreleri. J Clin Exp Invest. 2016;6(4):357-63.