The expression of Fetuin-A in brain tissues of WAG/Rij Rats, genetic rat model of absence epilepsy

Year 2015, , 387 - 390, 03.01.2016

Ramazan Yüksel , Gönül Gürol Zeynep Akkılık Sibel Sarı Sevil Arabacı Didem Güneri Kadir Demircan Fatih Ekici

https://doi.org/10.5799/ahinjs.01.2015.04.0554

Abstract

Objective: In the present study, we aimed to determine the Fetuin-A levels in different regions of the brain in absence epileptic Wistar Albino Glaxo/Rijswijk (WAG/Rij) rats in order to contribute the identification of new potential biomarkers of the diagnosis, prognosis and follow up the epilepsy treatment.

Methods: 1, 3 and 6 months old male WAG/Rij rats (n=21) with absence epilepsy were used in this study. All of the rats were decapitated under anesthesia and their cortex and thalamus tissues were isolated. Fetuin-A levels of the groups were determined by Western Blot method by using standard techniques and differences between densities of the groups were compared.

Results: According to data obtained, there was no Fetuin-A expression in brain cortex and thalamus tissues of WAG/Rij rats with absence epilepsy.

Conclusion: In this study, it was shown that Fetuin-A is not expressed in brain cortex and thalamus tissues of WAG/Rij rats with absence epilepsy throughout the age-related development. By evaluating the findings obtained, extensive researches that contain molecular and histological methods must be planned, Fetuin-A findings that are obtained experimentally must be confirmed. J Clin Exp Invest 2015; 6 (4): 387-390

Key words: Absence epilepsy, Fetuin-A, WAG/Rij rat

Keywords

Absans epilepsi, Fetuin-A, WAG/Rij sıçan

References

• 1. Devinsky O, Vezzani A, Najjar S, et al. Glia and epilepsy: excitability and inflammation. Trends Neurosci 2013;36:174-184.
• 2. Zupec-Kania BA, Spellman E. An overview of the ketogenic diet for pediatric epilepsy. Nutr Clin Pract 2009;23:589-596.
• 3. Vanlı Yavuz EN, Bebek N. Epilepsi tanı ve tedavisinde elektroensefalografinin (EEG) yeri. Klinik Gelişim 2010;23:35-38.
• 4. Sitnikova E, van Luijtelaar G. Cortical and thalamic coherence during spike-wave seizures in WAG/Rij rats. Epilepsy Res 2006;71:159-180
• 5. Sitnikova E, van Luijtelaar G. Electroencephalographic characterization of spike-wave discharges in cortex and thalamus in WAG/Rij rats. Epilepsia 2007;48:2296-2311.
• 6. Coenen AM, van Luijtelaar EL. Genetic animal models for absence epilepsy: a review of the WAG/Rij strain of rats. Behav Genet 2003;33:635-655.
• 7. Mori K, Emoto M, Inaba M. Fetuin-A and the cardiovascular system. Adv Clin Chem 2012; 56: 175-195.
• 8. Ma S, He Z, Zhao J, et al. Association of AHSG gene polymorphisms with ischemic stroke in a Han Chinese population. Biochem Genet 2013;51:916-926.
• 9. Dziegielewska KM, Mollgard K, Reynolds ML, Saunders NR. A Fetuin-related glycoprotein (alpha 2HS) in human embryonic and fetal development. Cell Tissue Res 1987;248:33-41.
• 10. Demircan K, Topcu V, Takigawa T, et al. ADAMTS4 and ADAMTS5 knockout mice are protected from versican but not aggrecan or brevican proteolysis during spinal cord injury. Biomed Res Int 2014;2014:693746.
• 11. Patel HC, Ross FM, Heenan LE, et al. Neurodegenerative actions of interleukin-1 in the rat brain are mediated through increases in seizure activity. J Neurosci Res 2006;83:385-391.
• 12. Akin D, Ravizza T, Maroso M, et al. IL-1β is induced in reactive astrocytes in the somatosensory cortex of rats with genetic absence epilepsy at the onset of spike-and-wave discharges, and contributes to their occurrence. Neurobiol Dis 2011;44:259-269.
• 13. Choi J, Koh S. Role of brain Inflammation in epileptogenesis. Yonsei Med J 2008;49: 1-18.
• 14. Vezzani A. Brain inflammation and seizures. Epilepsy Curr 2004;4:73-75.
• 15. Shandra AA, Godlevsky LS, Vastyanov RS, et al. The role of TNF-alpha in amygdala kindled rats. Neurosci Res 2002;42:147-153.
• 16. Eriksson C, Zou LP, Ahlenius S, et al. Inhibition of kainic acid induced expression of interleukin-1 beta and interleukin-1 receptor antagonist mRNA in the rat brain by NMDA receptor antagonists. Brain Res Mol Brain Res 2000;85:103-113.
• 17. Bambal G, Çakıl D, Ekici F. Models of experimental epilepsy J Clin Exp Invest 2011;2:118-123.
• 18. Luijtelaar G, Lyashenko S, Vastyanov R, et al. Cytokines and absence seizures in a genetic rat model. Neurophysiology 2012;43:478-486.
• 19. Bilgir F, Bilgir O, Kebapcilar L, et al. Soluble CD40 ligand, high sensitive C-reactive protein and fetuinA levels in patients with essential thrombocythemia. Transfus Apher Sci 2012;46:67-71.
• 20. Lim P, Moutereau S, Simon T, et al. Usefulness of fetuin-A and C-reactive protein concentrations for prediction of outcome in acute coronary syndromes (from the French Registry of Acute ST-Elevation NonST-Elevation Myocardial Infarction [FAST-MI]). Am J Cardiol 2013;111:31-37.
• 21. Smith ER, Nilforooshan R, Weaving G, Tabet N. Plasma fetuin-A is associated with the severity of cognitive impairment in mild-to-moderate Alzheimer’s disease. J Alzheimers Dis 2011;24:327-333.
• 22. Daveau M, Davrinche C, Djelassi N, et al. Partial hepatectomy and mediators of inflammation decrease the expression of liver alpha 2-HS glycoprotein gene in rats. FEBS Lett 1990;273:79-81.
• 23. Daveau M, Christian-Davrinche, Julen N, et al. The synthesis of human alpha-2-HS glycoprotein is downregulated by cytokines in hepatoma HepG2 cells. FEBS Lett 1988;241:191-194.
• 24. Harris VK, Donelan N, Yan QJ, et al. Cerebrospinal fluid fetuin-A is a biomarker of active multiple sclerosis. Mult Scler 2013;19:1462-1472.
• 25. Ottervald J, Franzén B, Nilsson K, et al. Multiple sclerosis: Identification and clinical evaluation of novel CSF biomarkers. J Proteomics 2010;73:1117-1132.
• 26. Puchades M, Hansson SF, Nilsson CL, et al. Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer’s disease. Brain Res Mol Brain Res 2003;118:140-146.