Detection of Acute Gastroenteritis Agents By Molecular Methods

Year 2018, , 21 - 25, 10.03.2018

Şafak Göktaş Ayşegül Aksoy Gökmen , Pınar Şamlıoğlu

https://doi.org/10.5799/jcei.413060

Abstract

Objective:
Gastroenteritis
is the most important cause of morbidity and mortality in all age groups all
over the world. Multiplex PCR tests give sensitive and specific results in the
investigation of bacterial, viral, parasitic agents. In this study, it was
aimed to determine the agents of the stool specimens of patients with acute
diarrhea by multiplex PCR.

Materials
and Methods: Stool
sample taken from 471 patients sent to Istanbul Gelişim Laboratories between
January 1, 2015 and September 30, 2016 was included in the study. All stool
samples were processed according to manufacturer’s instructions with
GastroFinder SMART 18 FAST multiplex PCR test (Pathofinder, Holland). 18
different gastrointestinal pathogens were diagnosed in one study.

Results:
Of the 471
patients stool sample included in the study. The agent was negative in 241
(51.2%), while the agent was isolated in 230 (48.8%). 190 (82%) had a single
pathogen, 40 had two or more pathogens. Of the 190 samples detected with single
agent, 149 (31.6%) were bacterial, 26 (5.5%) were parasitic and 15 (3.1%) were
viral agents.

Of the 149
bacterial agents, 108 (23%) was detected as Salmonella spp, 14 (6%) as
EHEC, 8 (3.5%) as Clostridium difficile toxin A / B, 8 (3.5%) as Campylobacter
spp., 7 (3%) Aeromonas spp., 2 (0.8%) Yersinia enterocolitica,
2 (0.8%) Enterotoxigenic E. coli (ETEC). Of 26 parasitic agents,
18 (7.8%) was detected as Giardia lamblia, 6 (2.6%) as Dientamoeba
fragilis and 2 (0.8%) as Cryptosporidium spp.

Conclusion:
Identification
of enteric pathogens by multiplex PCR will avoids the use of unnecessary
antibiotic treatments

Keywords

Gastroenteritis, Multiplex PCR, stool

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