The Association Between MGP Gene Polymorphisms and Coronary Artery Disease

Year 2016, Volume: 7 Issue: 3, 229 - 236, 02.09.2016

Mehmet Zihni Bilik Ali Fuad Kara Bülent Göğebakan Mehmet Ata Akıl , Ferhat Özyurtlu , Halit Acet , Sait Alan

https://doi.org/10.5799/jcei.328614

Abstract

Objectives: Coronary artery calcification (CAC) is associated with coronary atherosclerosis. There is a significant
relation between coronary artery wall calcification and coronary artery disease (CAD). The measure of coronary artery
calcification is an indicator of subclinical atherosclerosis. In some studies, it has been shown that coronary artery
calcification is independent from conventional cardiovascular risks, which suggests data about prognosis. Matrix Gla
protein (MGP) is an important protective modulator against calcification since it is an inhibitor of tissue calcification. In
this respect, we aimed to establish the relationship between the distributions of nucleotide alterations found in promoter
and coding regions of the MGP gene in patients with CAD and patients with normal coronary.
Methods: DNA samples (n=115) were obtained from 58 patients with CAD and 57 healthy controls. The DNA samples
obtained were analyzed by a Polymerase Chain Reaction (PCR) method using three sets of primer pairs, which cover
the coding (Thr83Ala in exon 4) and promoter regions (T-138C and G-7A) of the MGP gene. Amplified regions were
analyzed by a Restriction Fragment Length Polymorphism (RFLP) method for possible polymorphisms.
Results: The chi-square analysis of the results revealed that there is no relationship between the observed
polymorphisms and CAD.
Conclusions: In this study, we investigated the relationship between MGP gene polymorphism and CAD. However,
according to our findings, there was no statistically significant difference between the CAD and the control group. 

Keywords

Coronary artery disease, vascular calcification, matrix Gla Protein, genetic polymorphism

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