An Easy-to-Use, Rapid and Inexpensive Method to Determine Methicillin Resistance In Staphylococcus aureus

Year 2016, Volume: 7 Issue: 3, 225 - 251, 02.09.2016

Jülide Sedef Göçmen , Osman Çağlayan , Alpay Azap

https://doi.org/10.5799/jcei.328616

Abstract

Objectives: We aimed to report a new turbidimetric method to identify methicillin resistance in S. aureus strains just
two hours after identification of the microorganism, and to analyze diagnostic and discrimination abilities of this new
method.
Methods: A total of 319 S. aureus isolates were included in the study. Identification of bacteria was done by the colony
morphology, and conventional biochemical methods. The turbidimetric method we developed is based on different
growth rates of S. aureus in two media, with or without oxacillin. The growth rates of MRSA and MSSA are similar in
normal media; however, the MRSA grows significantly faster in the media containing oxacillin. Therefore, after 2 hours
of incubation, the difference of turbidity produced by bacteria in the media with or without oxacillin is less in case of
MRSA, and more in case of MSSA. The absorbances of the microplates were measured before incubation, and at 2nd
and 3rd hours of incubation. The “absorbance rate” was calculated for each bacteria based on those absorbance values
measured. The bacteria were classified as MRSA or MSSA based on the absorbance rate.
Results: All MRSA and MSSA strains were correctly discriminated via our turbidimetric method, when an absorbance
rate of 1.900 was taken as cut-off value. The new method could diagnose MRSA with 100% specificity and 100%
sensitivity in just 2 hours.
Conclusions: The turbidimetric method is a rapid, easy and cheap method that does not require any specific
equipment. It can be easily performed in every microbiology laboratory. 

Keywords

Turbidimetric method, MRSA, MSSA

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