The effects of propolis on cytokine production in lipopolysaccharide-stimulated macrophages

Year 2011, Volume: 2 Issue: 4, 366 - 370, 01.12.2011

Esma Gündüz Kaya Hatice Özbilge

https://doi.org/10.5799/ahinjs.01.2011.04.0073

Cited By: 1

Abstract

Objectives: Propolis, a bee-product, has attracted researchers\' interest in recent years because of several biological and pharmacological properties. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria and has an important role in the pathogenesis of septic shock and several inflammatory diseases by causing excessive release of inflammatory cytokines. The aim of this study was to investigate the effects of ethanol extract of propolis collected in Kayseri and its surroundings on production of pro-inflammatory cytokines in LPS-stimulated macrophages. Materials and methods: In vitro, U937 human macrophage cells were grown in RPMI-1640 medium supplemented with fetal bovine serum (10%) and penicillin-streptomycin (2%) and divided into: control, LPS treated, and propolis+LPS treated cell groups. After incubation in an atmosphere of 5% CO2 and at 37°C of cells, interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α levels were measured in cell-free supernatants by ELISA. Results: IL-1β, IL-6 and TNF-α levels increased in LPS treated cell group according to control, statistically significant. Each cytokine levels significantly decreased in LPS and propolis treated cell group according to only LPS treated cell group (p

Keywords

Lipopolysaccharide, macrophage, pro-inflammatory cytokine, propolis

The effects of propolis on cytokine production in lipopolysaccharide-stimulated macrophages

Abstract

Amaç: Propolis, çeşitli biyolojik ve farmakolojik özelliklerinden dolayı son yıllarda araştırmacıların ilgisini çeken bir arı ürünüdür. Lipopolisakkarit (LPS), Gram negatif bakterilerin dış membran komponenti olup, inflamatuar sitokinlerin aşırı üretimine yol açarak septik şok ve çeşitli inflamatuar hastalıkların patogenezinde önemli rol oynar. Bu çalışmada Kayseri ve çevresinden toplanan propolis\'in etanolik ekstraktının, LPS ile uyarılmış makrofajlarda pro-inflamatuar sitokinlerin üretimi üzerine etkisinin araştırılması amaçlanmıştır. Gereç ve yöntem: İn vitro olarak, fetal bovin serum (%10) ve penisilin-streptomisin (%2) ilaveli RPMI-1640 besiyerinde üretilen U937 insan makrofaj hücreleri, kontrol, LPS uygulanan ve propolis+LPS uygulanan hücreler olmak üzere üç gruba ayrıldı. Hücrelerin 37°C ve %5 CO2\'li atmosferde inkübasyonu sonunda, hücre süpernatantlarında interlökin (IL)-1β, IL-6 ve tümör nekrozis faktör (TNF)-α düzeyleri ELISA ile ölçüldü. Bulgular: LPS uygulanan hücre grubunda, IL-1β, IL-6 ve TNF-α düzeylerinde kontrole göre istatistiksel olarak anlamlı bir artış saptanırken, LPS ve propolis uygulanan hücre grubunda -yalnız LPS uygulanan hücre grubuna göre- her üç sitokin düzeyinde de anlamlı bir azalma olduğu tespit edildi (p

Keywords

Lipopolisakkarit, makrofaj, pro-inflamatuar sitokin, propolis

References

• Sforcin JM. Propolis and the immune system: a review. J Ethnopharmacol 2007; 113(1): 1-14.
• Bankova V, Castro SL, Marcucci MC. Propolis: recent advances in chemistry and plant origin. Apidologie 2000; 31(1): 3-15.
• Jin M, Iwamoto T, Yamada K, Satsu H, Totsuka M, Shi- mizu M. Effects of chondroitin sulfate and its oligosac- charides on toll-like receptor mediated IL-6 secretion by macrophage-like J774.1 cells. Biosci Biotechnol Biochem 2011; 75(7): 1-7.
• Wang CZ, Zhang Y, Li XD, et al. PPARγ agonist sup- presses TLR4 expression and TNF-α production in LPS stimulated monocyte leukemia cells. Cell Bio- chem Biophys 2011; 60(2): 167-72.
• Blonska M, Bronikowska J, Pietsz G, Czuba ZP, Schell- er S, Krol W. Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 macrophages. J Ethnopharmacol 2004; 91(1): 25-30.
• Park EH, Khang JH. Suppressive effects of propolis in rat adjuvant arthritis. Arch Pharm Res 1999; 22(4): 554-8.
• Mirzoeva OK, Calder PC. The effect of propolis and its components on eicosanoid production during the inflammatory response. Prostag Leukotr Ess 1996; 55(3): 441-9.
• Korish AA, Arafa MM. Propolis derivative inhibit the systemic inflammatory response and protect hepatic and neuronal cells in acute septic shock. Braz J Infect Dis 2011; 15(4): 332-8.
• Yasuda T. Hyaluronan inhibits akt, leading to nuclear factor-κB down-regulation in lipopolysaccharide stim- ulated U937 macrophages. J Pharmacol Sci 2011; 115(4): 509-15.
• Yasuda T. Hyaluronan inhibits cytokine production by lipopolysaccharide stimulated U937 macrophages through down-regulation of NF-κB via ICAM-1. In- flamm Res 2007; 56(2): 246-53.