Investigation of the Allelopathic Effect of Juglone and Walnut Leaf Extract on Weeds (Cynodon dactylon, Convolvulus arvensis, and Cirsium arvense) in Apricot orchards

Abstract

In this study, commercially purchased juglone and dried walnut leaf extracts were used to investigate the allelopathic effect of Cynodon dactylon, Convolvulus arvensis, and Cirsium arvense species commonly found in apricot orchards. A 10⁻³ M juglone liqour was prepared by melting juglone in autoclaved water. Under tissue culture conditions, seeds were placed on MS medium such as juglone or walnut leaf extract, while in vivo tests were performed in pots. Seed germination, root and shoot length, and dry weight were measured to determine allelopathic effects. Juglone and walnut leaf extract partly blocked germination and root development in tissue culture conditions, while this effect was restricted in ex- vitro conditions. The most pronounced inhibition was detected in the root growth of Convolvulus arvensis. These outcomes recommended that juglone and walnut leaf extract have potential as environmentally friendly alternatives to traditional herbicides.

Keywords

• juglone
• allelopathy.
• germination

Supporting Institution

TUBİTAK

Project Number

2209-A

Thanks

This study was supported by the Scientific and Technological Research Council of Türkiye (TÜBİTAK) under the 2209-A Research Project Support Programme for Undergraduate Students.

Juglone ve Ceviz Yaprağı Ekstraktının Kayısı Bahçelerindeki Yabancı Otlar (Cynodon dactylon, Convolvulus arvensis ve Cirsium arvense) Üzerindeki Allelopatik Etkisinin Araştırılması

Abstract

Bu çalışmada, kayısı bahçelerinde yaygın olarak bulunan Cynodon dactylon, Convolvulus arvensis ve Cirsium arvense türlerinin allelopatik etkisini araştırmak için ticari olarak satın alınan juglon ve kurutulmuş ceviz yaprağı ekstraktları kullanılmıştır. Juglon otoklavlanmış suda eritilerek 10-³ M juglon likörü hazırlanmıştır. Doku kültürü koşullarında, tohumlar juglon veya ceviz yaprağı ekstraktı içeren MS ortamına yerleştirilirken, in vivo testler saksılarda gerçekleştirilmiştir. Allelopatik etkileri belirlemek için tohum çimlenmesi, kök ve sürgün uzunluğu ve kuru ağırlık ölçülmüştür. Juglon ve ceviz yaprağı ekstraktı doku kültürü koşullarında çimlenmeyi ve kök gelişimini kısmen engellerken, ex- vitro koşullarda bu etki sınırlı kalmıştır. En belirgin inhibisyon Convolvulus arvensis'in kök büyümesinde tespit edilmiştir. Bu sonuçlar, juglon ve ceviz yaprağı ekstraktının geleneksel herbisitlere çevre dostu alternatifler olma potansiyeline sahip olduğunu göstermiştir.

Keywords

• çimlenme
• juglon
• allopati

Supporting Institution

TÜBİTAK

Project Number

2209-A

Thanks

Bu çalışma, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) 2209-A – Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı kapsamında desteklenmiştir.

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