Borik Asit Uygulanan Farklı Hasat Dönemlerindeki Yeşil Çay Yapraklarının Biyokimyasal Değişikliklerinin İncelenmesi

Abstract

C. sinensis yapraklarından elde edilen çayın antioksidatif, pro-oksidatif ve antikarsinojenik gibi değişik farmakolojik etkileri vardır. Bitkilerin büyüme ve gelişmesi için gereksinim duyulan bor elementinin eksikliği reaktif oksijen türlerini meydana getirir. Yüksek bor konsantrasyonunun antioksidan enzim aktivitesini arttırdığı ve membran fonksiyonlarını reaktif oksijen türlerinin zararlarından koruduğu bilinmektedir. Çalışmamızın amacı; borik asit uygulanan yeşil çayın farklı dönemlerde hasat edilen yapraklarındaki biyokimyasal değişikliklerin incelenmesidir. Rize-Çayeli ilçesinde Çaykur deneme bahçesi 20 parsele ayrıldı. Gruplar, C. sinensis’e 0 (A grubu), 100 (B grubu), 300 (C grubu) ve 500 (D grubu) mg/m² konsantrasyonlarda borik asit uygulanarak oluşturuldu. Üç farklı hasat döneminde yeşil çay yaprakları toplandı ve sıvı nitrojende fikse edildi. Yeşil çay ekstrelerinden katalaz (KAT), süperoksit dismutaz (SOD) enzim aktivitelerinin ve glutatyon (GSH), malondialdehit (MDA) seviyelerinin tayini yapıldı. SOD enzim aktivitesi ve GSH seviyesi özellikle 3. hasat döneminde yüksekti, ayrıca B ve D gruplarında A grubuna göre anlamlı artış vardı (p < 0.05). MDA seviyesinde 1. hasat döneminde B, C ve D gruplarında, A grubuna göre bir azalma görüldü (p < 0.01). Bulgularımıza göre,  yeşil çay yapraklarının yetiştiği topraklarda meydana gelen bor eksikliği çayın koruyucu özelliğini düşürebilir. Bor uygulanan bölgelerden elde edilen çay yaprakları ile antioksidan özelliğine sahip daha kaliteli ürünler elde edilebilir.

Keywords

• Borik asit,yeşil çay,C. Sinensis,oksidatif stres

Investigation of Biochemical Changes of Green Tea Leaves Treated with Boric Acid in Different Harvest Periods

Abstract

Tea obtained from C. sinensis leaves has different pharmacological effects such as antioxidative, pro-oxidative and anticarcinogenic. The lack of boron elements required for the growth and development of plants causes the production of reactive oxygen species. It has been reported that high boron concentration also increases the antioxidant enzyme activity and protects the membrane functions from the damage of reactive oxygen species. The aim of this study was to investigate the biochemical changes of green tea leaves treated with boric acid in different harvest periods. Çaykur trial garden was divided into 20 parcels in Rize-Çayeli district. Groups were formed by applying boric acid to C. sinensis at concentrations of 0 (group A), 100 (group B), 300 (group C) and 500 (group D) mg/m². During three harvests, green tea leaves were collected and fixed in liquid nitrogen. The catalase (CAT), superoxide dismutase (SOD) enzyme activities and glutathione (GSH), malondialdehyde (MDA) levels of green tea extracts were determined. SOD enzyme activity and GSH level were high especially in the 3rd harvest period, and also B and D groups were significantly higher than A group (p <0.05). There was a decrease in the B, C and D groups at the MDA level in the 1st harvest period compared to the A group (p <0.01). According to our findings, the lack of boric acid in the soil where green tea leaves grow can reduce the protective properties of tea. Better quality products with antioxidant properties can be provided with tea leaves obtained from boric acid applied areas.

Keywords

• Boric acid,green tea,C. sinensis,oxidative stress

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