Antibiotic Resistance Profiles of Enterobacteriacea Strains Isolated from Kilis State Hospital Sewage Water

Abstract

Enterobacteria strains were isolated from the sewage water of Kilis State Hospital by using MacConkey medium. A total of 30 bacterial strains were selected according to their lactose degradation properties. The selected strains were identified by their morphological characteristics and biochemical activities. Association with the biochemical tests, 20 strains including Escherichia coli (Biotype I, II), Klebsiella spp and Citrobacter spp strains were partially identified. Antibiotic resistance profiles were determined by selecting 8 strains with different activities from 20 isolates. 11 antibiotics with different effect mechanisms were tested against the selected 8 bacterial strains (clindamycin, trimethoprim, chloramphenicol, erythromycin, tetracycline, streptomycin, ampicillin, cefotaxime, vancomycin, gentamicin and cefoperazone). The MAR index for all tested strains was found to be over 0.2 and the highest MAR index was 0.75 for Escherichia coli Biotype I and Citrobacter spp.

Keywords

• Sewage water,Antibiotic resistance,MAR index,Enterobacteriaceae

Kilis Devlet Hastanesi Kanalizasyon Suyundan İzole Edilen Enterobacteriacea Suşlarının Antibiyotik Dirençlilik Profilleri

Abstract

Kilis Devlet Hastanesi kanalizasyon suyundan MacConkey besi ortamı kullanılarak Enterobacteria familyasına ait suşlar izole edilmiştir. Laktozu parçalama özelliklerine göre toplamda 30 bakteri suşu seçilmiştir. Seçilen suşlar morfolojik karakterleri ve biyokimyasal aktivitelerine göre tanımlanmıştır. Biyokimyasal test sonucuna göre Escherichia coli (Biyotip I ve II)-(Ara tip I ve II), Klebsiella spp. ve Citrobacter spp. suşları içeren 20 suşun kısmi tiplendirilmesi yapılmıştır. Tiplendirilen 20 suştan farklı aktiviteler gösteren 8 suş seçilerek antibiyotik dirençlilik profilleri belirlenmiştir. Seçilen 8 bakteri suşu için farklı etki mekanizmalarına sahip 11 antibiyotik test edilmiştir (klindamisin, trimethoprim, kloramfenikol, eritromisin, tetrasiklin, streptomisin, amfisilin, sefotaksim, vankomisin, gentamisin ve sefoperazon). Test tüm suşlar için MAR indeksi 0.2’nin üzerinde olduğu bulunmuş olup, en yüksek ÇAD indeksi Escherichia coli Biyotip I ve Citrobacter spp suşlarında 0.75 olarak hesaplanmıştır.

Keywords

• Kanalizasyon suyu,Antibiyotik dirençlilik,Enterobacteriaceae,ÇAD indeksi

References

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