Antibiotic Resistance Profiles of Enterobacteriacea Strains Isolated from Kilis State Hospital Sewage Water

Year 2019, Volume: 31 Issue: 3, 261 - 266, 01.09.2019

Hatice Aysun Mercimek Takcı , Pemra Bakirhan Bakırhan , Melike Kaptanoglu Sema Genç

https://doi.org/10.7240/jeps.589186

Abstract

Enterobacteria strains were isolated from the
sewage water of Kilis State Hospital by using MacConkey medium. A total of 30 bacterial strains were selected
according to their lactose degradation properties. The selected strains were
identified by their morphological characteristics and biochemical activities. Association
with the biochemical tests, 20 strains including Escherichia coli (Biotype I, II), Klebsiella spp and Citrobacter
spp strains were partially identified. Antibiotic resistance profiles were
determined by selecting 8 strains with different activities from 20 isolates. 11
antibiotics with different effect mechanisms were tested against the selected 8
bacterial strains (clindamycin, trimethoprim, chloramphenicol, erythromycin,
tetracycline, streptomycin, ampicillin, cefotaxime, vancomycin, gentamicin and cefoperazone). The MAR index for all tested strains was found to be over 0.2 and the
highest MAR index was 0.75 for Escherichia
coli Biotype I and Citrobacter
spp.

Keywords

Sewage water, Antibiotic resistance, MAR index, Enterobacteriaceae

Kilis Devlet Hastanesi Kanalizasyon Suyundan İzole Edilen Enterobacteriacea Suşlarının Antibiyotik Dirençlilik Profilleri

Abstract

Kilis
Devlet Hastanesi kanalizasyon suyundan MacConkey besi ortamı kullanılarak Enterobacteria familyasına ait suşlar
izole edilmiştir. Laktozu parçalama özelliklerine göre toplamda 30 bakteri suşu
seçilmiştir. Seçilen suşlar morfolojik karakterleri ve biyokimyasal
aktivitelerine göre tanımlanmıştır. Biyokimyasal test sonucuna göre Escherichia coli (Biyotip I ve II)-(Ara
tip I ve II), Klebsiella spp. ve Citrobacter spp. suşları içeren 20 suşun
kısmi tiplendirilmesi yapılmıştır. Tiplendirilen 20 suştan farklı aktiviteler
gösteren 8 suş seçilerek antibiyotik dirençlilik profilleri belirlenmiştir. Seçilen
8 bakteri suşu için farklı etki mekanizmalarına sahip 11 antibiyotik test
edilmiştir (klindamisin, trimethoprim, kloramfenikol, eritromisin, tetrasiklin,
streptomisin, amfisilin, sefotaksim, vankomisin, gentamisin ve sefoperazon). Test
tüm suşlar için MAR indeksi 0.2’nin üzerinde olduğu bulunmuş olup, en yüksek ÇAD
indeksi Escherichia coli Biyotip I ve
Citrobacter spp suşlarında 0.75
olarak hesaplanmıştır.

Keywords

Kanalizasyon suyu, Antibiyotik dirençlilik, Enterobacteriaceae, ÇAD indeksi

References

• Referans1 Wan Ishak, W.M.F., Jamek S., Jalanni N.A. and Mohd Jamaludin, N.F. (2011). Isolation and Identification of Bacteria from Activated Sludge and Compostfor Municipal Solid Waste Treatment System. International Proceedings of Chemical. Biological and Environmental Engineering, 24: 450-454.
• Referans2 Mahesh, S., Aleem Basha, P. and Kavitha B. (2017). Isolation and Characterization of Bacteria Izolated From Municipal Sewage Water of Nandyal Kurnool, A.P., India. Asian Jr. of Microbiol. Biotec. Env. Sc., 19(3): 772-777.
• Referans3 Kılıç, Ü. (2018). Karbapenemaz Üreten Enterobactericea Kökenlerinde Çeşitli Antibiyotik Kombinasyonlarının in vitro Etkinliklerinin Araştırılması. Uzmanlık tezi, Sakarya Üniversitesi, Türkiye, 81.
• Referans4 Halipçi Topsakal, H.N. (2018). İnvazif Enfeksiyonlara Neden Olan Genişlemiş Spektrumlu Beta-Laktamaz (GSBL) Oluşturan Enterobacteriaceae İzolatlarının Hızlı Teşhisi için DNA Aptamerlerinin Geliştirilmesi. Doktora tezi, Kahramanmaraş Sütçü İmam Üniversitesi, Türkiye, 106.
• Referans5 Abdünnur, V. (2016). İstanbul’da Satışa Sunulan Dondurmaların Listeria monocytogenes ve Enterobacteriaceae Varlığı Yönünden İncelenmesi. Yüksek Lisans Tezi, İstanbul Aydın Üniversitesi, Türkiye, 107.
• Referans6 Elal Mus, T. and Cetinkaya, F. (2015). Investigation of the Presence of Indicator and Some Pathogenic Bacteria in Drinking and Usage Waters in Bursa. Soil Water Journal, 6(1): 1-6.
• Referans7 Kurt, A.Ö., Şaşmaz, Tç, Buğdaycı, R., Öner, S. ve Kızılok, A. (2009). Mersin ili içme kullanma suyu numunelerinin bakteriyolojik yönden değerlendirilmesi. Türkiye Halk Sağlığı Dergisi, 7(1): 23-31.
• Referans8 Avcı, S., Bakıcı, M.Z. ve Erandaç, M. (2016). A Research on Drinking Water in Tokat in Terms of Coliform Bacteria. C.Ü Tıp Fakültesi Dergisi, 28(4): 107-112
• Referans9 Behesheti Maal, K., Soleimani Delfan, A. and Salmanizadeh, S. (2015). Isolation and Identification of Two Novel Escherichia coli Bacteriophages and Their Application in Wastewater Treatment and Coliform's Phage Therapy. Jundishapur J Microbiol., 8(3): e14945
• Referans10 Zwenger, S.R. and Gillock ,E.T. (2009). Bacteria Isolated from Sewage Influent Resistant to Ciprofloxacin, Chloramphenicol and Tetracycline. J Environ Sci Health A, 44: 123-129.
• Referans11 Reinthaller, F.F., Posch, J., Feierl, G., Wüst, G., Haas, D., Ruckenbauer, G., Mascher, F. and Marth, E. (2003). Antibiotic resistance of E. coli in sewage and sludge. Water Res., 37: 1685-1690.
• Referans12 Alam, M.Z., Aqil, F., Ahmad, I. and Ahmad, S. (2013). Incidence and Transferability of AntibioticRresistance in the Enteric Bacteria Isolated from Hospital Waste Water. Braz. J. Microbiol., 44(3): 799-806
• Referans13 Osińska, A., Korzeniewska, E., Harnisz, M. and Nestҿpski, S. (2019). Quantitative Occurrence of Antibiotic Resistance Genes among Bacterial Populations from Wastewater Treatment Plants Using Activated Sludge. Appl. Sci., 9(387): 1-15.
• Referans14 Bolaji, A.S., Akande, I.O., Iromini, F.A., Adewoye, S.O. and Opasola, O.A. (2011). Antibiotic resistance pattern of bacteria spp isolated from hospital waste water in Ede South Western, Nigeria. Euro J Exp Bio., 1(4):66-71.
• Referans15 Amaya, E., Reyes, D., Paniagua, M., Calderόn, S., Rashid, M.U., Colque, P., Kühn, I., Möllby, R., Weintraub, A. and Nord, C.E. (2012). Antibiotic Resistance Patterns of Escherichia Coli Isolates from Different Aquatic Environmental Sources in Leόn, Nicaragua. Clin. Microbiol. Infect., E347–E354
• Referans16 Yang, C.M., Lin, M.F., Liao, P.C., Yeh, H.W., Chang, B.V., Tang, T.K., Cheng, C. Sung, C.H. and Liou, M.L. (2009). Comparison of Antimicrobial Resistance Patterns Between Clinical and Sewage Isolates in a Regional Hospital in Taiwan. Lett. Appl. Microbiol.. 48: 560-565.
• Referans17 Moges, F., Endris, M., Belyhun, Y. and Worku, W. (2014). Isolation and characterization of multiple drug resistance bacterial pathogens from waste water in hospital and non-hospital environments, Northwest Ethiopia. BMC Research Notes 7(215): 1-6.
• Referans18 Pandey, A., Afsheen, Ara, F. and Tiwari, S.K. (2011). Isolation and Characterization of Multi Drug Resistance Cultures from Waste Water. J. Pharm. Biomed. Sci., (JPBMS) 13(14): 1-15.