Hidroksiapatit / seryum oksit kompozitleri: Sinterleme, mikroyapısal, mekanik ve invitro biyoaktivite özellikleri

Year 2019, Volume: 31 Issue: 4, 295 - 304, 01.11.2019

Süleyman Serdar Pazarlıoğlu

https://doi.org/10.7240/jeps.512240

Cited By: 2

Abstract

Effect of cerium
oxide (CeO₂) additive on the microstructure, mechanical and invitro
bioactivity properties of a commercially synthetic hydroxyapatite (HA) was
investigated. HA without CeO₂ started to decompose at 1100 ^oC,
but the decomposition temperature of the CeO₂ added samples
decreased up to 900 ^oC. Decomposition rate of the sintered samples
increased as the sintering temperature reached to 1300 ^oC. It was
about 5.8% for monolithic HA, and increased to 11.4% when the CeO₂
additive to HA reached to 2.5 wt%. SEM images showed that an excessive grain
growth as well as microcracks occured on the surface of monolithic HA when it
was sintered at the temperatures than that of 1100 ^oC. The
microcracks were also observed on the surface of HA-CeO₂ composites,
when they were sintered at 1300 ^oC. The composite of HA-0.5CeO₂
sintered at 1100 ^oC possess the higher fracture toughness (Kıc)
(2.510 ± 0.225 MPam^1/2) and the higher compressive strength (152.73
± 6.31 MPa) compared to other HA-CeO₂ composites, and it's
mechanical properties are higher than that of monolithic HA at about 2-3 times.
In-vitro bioactivity test results showed that apatite layers on the surface of
the samples were in the different morphologies. 

Keywords

Hidroksiapatit, Sinterleme, Seryum

Hydroxyapatite/cerium oxide composites: Sintering, microstructural, mechanical and invitro bioactivity properties

Abstract

Effect of cerium
oxide (CeO₂) additive on the microstructure, mechanical and invitro
bioactivity properties of a commercially synthetic hydroxyapatite (HA) was
investigated. HA without CeO₂ started to decompose at 1100 ^oC,
but the decomposition temperature of the CeO₂ added samples
decreased up to 900 ^oC. Decomposition rate of the sintered samples
increased as the sintering temperature reached to 1300 ^oC. It was
about 5.8% for monolithic HA, and increased to 11.4% when the CeO₂
additive to HA reached to 2.5 wt%. SEM images showed that an excessive grain
growth as well as microcracks occured on the surface of monolithic HA when it
was sintered at the temperatures than that of 1100 ^oC. The
microcracks were also observed on the surface of HA-CeO₂ composites,
when they were sintered at 1300 ^oC. The composite of HA-0.5CeO₂
sintered at 1100 ^oC possess the higher fracture toughness (Kıc)
(2.510 ± 0.225 MPam^1/2) and the higher compressive strength (152.73
± 6.31 MPa) compared to other HA-CeO₂ composites, and it's
mechanical properties are higher than that of monolithic HA at about 2-3 times.
In-vitro bioactivity test results showed that apatite layers on the surface of
the samples were in the different morphologies. 

Keywords

Hydroxyapatite, cerium oxide, Sintering

References

• [1] Youness, R.A., Taha, M.A., Ibrahim, M.A. (2017). Effect of sintering temperatures on the in vitro bioactivity, molecular structure and mechanical properties of titanium/carbonated hydroxyapatite nanobiocomposites. J. Mol. Struct. 1150, 188-195.
• [2] Khanal, S.P., Mahfuz, H., Rondinone, A.J., Leventouri, Th. (2016). Improvement of the fracture toughness of hydroxyapatite (HAp) by incorporation of carboxyl functionalized single walled carbon nanotubes (CfSWCNTs) and nylon. Mater. Sci. Eng., C 60, 204-210.
• [3] Yetmez, M., Erkmen, Z.E., Kalkandelen, C., Ficai, A., Oktar, F.N. (2017). Sintering effects of mullite-doping on mechanical properties of bovine hydroxyapatite. Mater. Sci. Eng., C 77 , 470-475.
• [4] Castkova, K., Hadraba, H., Matousek, A., Roupcova, P., Chlup, Z., Novotna, L., Cihlar, J. (2016). Synthesis of Ca,Y-zirconia/hydroxyapatite nanoparticles and composites. J. Eur. Ceram. Soc. 36, 2903-2912.
• [5] Brzezińska-Miecznik, J., Haberko, K., Sitarz, M., Bućko, M.M., Macherzyńska, B., Lach, R. (2016). Natural and synthetic hydroxyapatite/zirconia composites: A comparative study. Ceram. Int. 42, 11126-11135.
• [6] Evis, Z., Doremus, R.H. (2008). Effect of AlF3, CaF2 and MgF2 on hot-pressed hydroxyapatite-nanophase alpha-alumina composites. Mater. Res. Bull. 43, 2643-2651
• [7] Kutbay, I., Yilmaz, B., Evis, Z., Usta, M. (2014). Effect of calcium fluoride on mechanical behavior and sinterability of nano-hydroxyapatite and titania composites. Ceram. Int. 40, 14817-14826.
• [8] Zhou, Y., Rahaman, M.N. (1997). Effect of redox reaction on the sintering behavior of cerium oxide. Acta Mater. 45(9), 3635-3639.
• [9] Wang, X., Deng, L.L., Wang, L.Y., Dai, S.M., Xing, Z., Zhan, X.X., Lu, X.Z., Xie, S.Y., Huang, R.B., Zheng, L.S. (2017) . Cerium oxide standing out as an electron transport layer for efficient and stable perovskite solar cells processed at low temperature. J. Mater. Chem. A 5, 1706-1712.
• [10] Nakane, S., Tachi, T., Yoshinaka, M., Hirota, K., Yamaguchi, O. (1997). Characterization and sintering of reactive cerium(IV) oxide powders prepared by the hydrazine method. J. Am. Ceram. Soc. 80(12), 3221-3224.
• [11] Yan, B., Zhang, Y., Chen, G., Shan, R., Ma, W., Liu, C. (2016). The utilization of hydroxyapatite-supported CaO-CeO2 catalyst for biodiesel production. Energ. Conver. Manage. 130, 156-164.
• [12] Patil, S., Sandberg, A., Heckert, E., Self, W., Seal, S. (2007). Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential. Biomater. 28, 4600-4607.
• [13] Hirst, S.M., Karakoti, A.S., Tyler, R.D., Sriranganathan, N., Seal, S., Reilly, C.M. (2009). Anti-inflammatory properties of cerium oxide nanoparticles. Small 5(24), 2848-2856.
• [14] Gopi, D., Murugan, N., Ramya, S., Shinyjoy, E., Kavitha, L. (2015). Ball flower like manganese, strontium substituted hydroxyapatite/cerium oxide dual coatings on the AZ91 Mg alloy with improved bioactive and corrosion resistance properties for implant applications. RSC Adv. 5, 27402-27411.
• [15] Li, K., Yu, J., Xie, Y., You, M., Huang, L., Zheng, X. (2017). The effects of cerium oxide incorporation in calcium silicate coating on bone mesenchymal stem cell and macrophage responses. Biol. Trace Elem. Res. 177, 148-158.
• [16] Ivanchenko, L.A., Pinchuk, N.D., Parkhomei, A.R., Golovkova, M.E., Molchanovskaya, M.I., Syabro, A.N. (2009). Effect of cerium dioxide on the properties of biogenic hydroxyapatite sintered with borosilicate glass. Powder Metall. Met. Ceram. 48 (5-6), 305-310.
• [17] Gunduz, O., Gode, C., Ahmad, Z., Gökçe, H., Yetmez, M., Kalkandelen, C., Sahin, Y.M., Oktar, F.N. (2014). Preparation and evaluation of cerium oxide-bovine hydroxyapatite composites for biomedical engineering applications. J. Mech. Behav. Biomed. Mater. 35, 70-76.
• [18] Pazarlioglu, S., Salman, S. (2017). Sintering effect on the microstructural, mechanical, and in vitro bioactivity properties of a commercially synthetic hydroxyapatite. J. Aust. Ceram. Soc. 53, 391-401.
• [19] Majling, J., Znáik, A., Palová, S., Stevĭk, S., Kovalĭk, D.K., Roy, A.R. (1997). Sintering of the ultrahigh pressure densified hydroxyapatite monolithic xerogels. J. Mater. Res. 12(1), 198-202.
• [20] Ozawa, M. (2004). Effect of oxygen release on the sintering of fine CeO2 powder at low temperature. Scripta Mater. 50, 61-64.
• [48] Chen, M., Lu, C., Yu, J. (2007). Improvement in performance of MgO-CaO refractories by addition of nano-sized ZrO2. J. Eur. Ceram. Soc. 27, 4633-4638.
• [22] Ruys, A.J., Wei, M., Sorrell, C.C., Dickson, M.R., Brandwood, A., Milthorpe, B.K. (1995). Sintering effects on the strength of hydroxyapatite. Biomater.16, 409-415.
• [23] Fathi, M.H., Hanifi, A., Mortazavi, V. (2008). Preparation and bioactivity evaluation of bone-like hydroxyapatite nanopowder. J. Mater. Process. Technol. 202, 536-542.
• [24] Wang, A.J., Lu, Y.P., Zhu, R.F., Li, S.T., Xiao, G.Y., Zhao, G.F., Xu, W.H. (2008). Effect of sintering on porosity, phase, and surface morphology of spray dried hydroxyapatite microspheres. J. Biomed. Mater. Res. A 87A(2), 557-562.
• [25] Dorozhkin, S.V. (2008). Green chemical synthesis of calcium phosphate bioceramics. J. Appl. Biomater. Biomech. 6(2), 104-109.
• [26] Mateus, A.Y.P., Barrias, C.C., Ribeiro, C., Ferraz, M.P., Monteiro, F.J. (2008). Comparative study of nanohydroxyapatite microspheres for medical applications. J. Biomed. Mater. Res. A 86(2), 483-493.
• [27] Locardi, B., Pazzaglia, V.E., Gabbi, C., Profilo, B. (1993). Thermal behaviour of hydroxyapatite intended for medical applications. Biomater. 44, 437-441.
• [28] Muralithran, G., Ramesh, S. (2000). The effects of sintering temperature on the properties of hydroxyapatite. Ceram. Inter. 26, 221-230.
• [29] Hull, S., Norberg, S.T., Ahmed, I., Eriksson, S.G., Marrocchelli, D., Madden, P.A. (2009). Oxygen vacancy ordering within anion-deficient ceria. J. Solid State Chem. 182, 2815-2821.
• [30] Zinkevich, M., Djurovic, D., Aldinger, F. (2006). Thermodynamic modelling of the cerium-oxygen system. Solid State Ionics 177, 989-1001.
• [31] Kümmerle, E.A., Heger G. (1999). The Structures of C-Ce2O3+δ, Ce7O12, and Ce11O20. J. Solid State Chem. 147, 485-500.
• [32] Morais, D.S., Fernandes, S., Gomes, P.S., Fernandes, M.H., Sampaio, P., Ferraz, M.P., Santos, J.D., Lopes, M.A., Hussain, N.S. (2015). Novel cerium doped glass-reinforced hydroxyapatite with antibacterial and osteoconductive properties for bone tissue regeneration. Biomed. Mater. 10(5), 055008.
• [33] Gamoke, B., Neff, D., Simons, J. (2009). Nature of PO bonds in phosphates. J. Phys. Chem. A 113, 5677-5684.
• [34] Frayssinet, P., Rouquet, N., Fages, J., Durand, M., Vidalain, P.O., Bonell, G. (1997). The influence of sintering temperature on the proliferation of fibroblastic cells in contact with HA-bioceramics. J. Biomed. Mater. Res. 35, 337-347.
• [35] Fanovich, M.A., Castro, M.S., Lȯpez, J.M.P. (1998). Improvement of the microstructure and microhardness of hydroxyapatite ceramics by addition of lithium. Mater. Lett. 33, 269-272.
• [36] Habibovic, P., Yuan, H., van der Valk, C.M., Meijer, G., van Blitterswijka, C.A., de Groot, K. (2005). 3D micro environment as essential element for osteoinduction by biomaterials. Biomater. 26, 3565-3575
• [37] Chu, C., Lin, P., Xue, X., Zhu, J., Yin, Z. (2002). Fabrication and characterization of hydroxyapatite reinforced with 20 vol% Ti particles for use as hard tissue replacement. J. Mater. Sci. Mater. Med. 13, 985-992.
• [38] Li, X.W., Yasuda, H.Y., Umakoshi, Y. (2006). Bioactive ceramic composites sintered from hydroxyapatite and silica at 1200oC: preparation, microstructures and in vitro bone-like layer growth. J. Mater. Sci. Mater. Med. 17, 573-581.
• [39] Evis, Z. (2007). Reactions in hydroxylapatite-zirconia composites, Ceram. Inter. 33, 987-991.
• [40] Upasani, M. (2017). Synthesis of Y3Al5O12:Tb & Y3Al5O12:Tb,Si phosphor by combustion synthesis: Comparative investigations on the structural and spectral properties. Opt. Mater. 64, 70-74
• [41] Mikhailov, M.M., Vlasov, V.A., Yuryev, S.A., Neshchimenko, V.V., Shcherbina, V.V. (2015). Optical properties and radiation stability of TiO2 powders modified by Al2O3, ZrO2, SiO2, TiO2, ZnO, and MgO nanoparticles. Dyes and Pigments 123, 72-77.
• [42] Tan, C.Y., Yaghoubi, A., Ramesh, S., Adzila, S., Purbolaksono, J., Hassan, M.A., Kutty, M.G. (2013). Sintering and mechanical properties of MgO-doped nanocrystalline hydroxyapatite. Ceram. Inter. 39, 8979-8983.
• [43] Hoepfner, T.P., Case, E.D. (2004). An estimate of the critical grain size for microcracks induced in hydroxyapatite by thermal expansion anisotropy. Mater. Lett. 58, 489- 492.
• [44] Case, E.D., Smith, I.O., Baumann, M.J. (2005). Microcracking and porosity in calcium phosphates and the implications for bone tissue engineering. Mater. Sci. Eng. A 390, 246-254.
• [45] Miao, X., Chen, Y., Guo, H., Khor, K.A. (2004). Spark plasma sintered hydroxyapatite-yttria stabilized zirconia composites. Ceram. Inter. 30, 1793-1796.
• [46] Sameshima, S., Kawaminami, M., Hirata, Y. (2002). Thermal expansion of rare-earth-doped ceria ceramics, J. Ceram. Soc. Jpn 110(7), 597-600.
• [47] Ruseska, G., Fidancevska, E., Bosser, J. (2006). Mechanical and thermal-expansion characteristics of Ca10(PO4)6(OH)2-Ca3(PO4)2 composites. Science of Sintering 38, 245-253.
• [49] Hassan, M.N., Mahmoud, M.M., Link, G., El-Fattah, A.A., Kandil, S. (2016). Sintering of naturally derived hydroxyapatite using high frequency microwave processing. J. Alloys Compd. 682, 107-114.
• [50] Herliansyah, M.K., Hamdi, M., Ide-Ektessabi, A., Wildan, M.W., Toque, J.A. (2009). The influence of sintering temperature on the properties of compacted bovine hydroxyapatite. Mater. Sci. Eng. C 29, 1674-1680.
• [51] Wang, P.E., Chaki, T.K. (1993). Sintering behaviour and mechanical properties of hydroxyapatite and dicalcium phosphate. J. Mater. Sci. Mat. Med. 4, 150-158.
• [52] Rootare, H.M., Craig, R.G. (1974). Characterization of the compaction and sintering of hydroxyapatite powders by mercury porosimetry, Powder Technol. 9, 199-211.
• [57] Hoepfner, T.P., Case, E.D. (2003). The influence of the microstructure on the hardness of sintered hydroxyapatite. Ceram. Inter. 29, 699-706.
• [54] Wang, X., Fan, H., Xiao, Y., Zhang, X. (2006) . Fabrication and characterization of porous hydroxyapatite/β-tricalcium phosphate ceramics by microwave sintering. Mater. Lett. 60, 455-458.
• [55] Alymov, M.I., Bakunova, N.V., Barinov, S.M., Belunik, I.A., Fomin, A.S., Ievlev, V.M., Soldatenko, S.A. (2011). Specific features of the densification of hydroxyapatite nanopowders upon pressing. Nanotechnologies in Russia 6(5-6), 353-356.
• [56] Pazarlioglu, S.S., Gokce, H., Ozyegin, L.S., Salman, S. (2014). Effect of sintering on the microstructural and mechanical properties of meleagris gallopova hydroxyapatite. Bio-Med. Mater. Eng. 24, 1751-1769.
• [58] Wang, J., Shaw, L.L. (2009). Nanocrystalline hydroxyapatite with simultaneous enhancements in hardness and toughness. Biomater. 30, 6565-6572.
• [59] Ryu, H.S., Youn, H.J., Hong, K.S., Chang, B.S., Lee, C.K., Chung, S.S. (2002). An improvement in sintering property of β-tricalcium phosphate by addition of calcium pyrophosphate. Biomater. 23, 909-914.
• [60] Mansour, S.F., El-dek, S.I., Ahmed, M.K. (2017). Physico-mechanical and morphological features of zirconia substituted hydroxyapatite nano crystals. Sci. Rep. 7, 43202.
• [61] Zhu, Q., Ablikim, Z., Chen, T., Cai, Q., Xia, J., Jiang, D., Wang, S. (2017). The preparation and characterization of HA/β-TCP biphasic ceramics from fish bones. Ceram. Inter. 43, 12213-12220.