Evaluation of High Concentrations of Sugar Beet Molasses as Substrate for Hydrogen and 5-Aminolevulinic Acid Productions

Year 2020, Volume: 32 Issue: 4, 398 - 404, 01.11.2020

Gökhan Kars , Ümmühan Alparslan

https://doi.org/10.7240/jeps.647523

Cited By: 1

Abstract

Sugar beet molasses is a valuable raw material and it contains high
amount of sugar especially sucrose. Therefore, it could be used as substrate
for the generation of highly valuable chemicals by microorganisms. Here,
considerably high concentrations of molasses were tested for the first time to
investigate if they could enhance the growth of Rhodobacter sphaeroides O.U.001
and generations of hydrogen and 5-aminolevulinic acid (5-ALA). Firstly, five
distinct growth cultures having sugar contents of 34 g/L, 41 g/L, 48 g/L, 55 g/L and 61 g/L were made ready using molasses. Then,
in batch processes, bacterial growth and generations of hydrogen and 5-ALA were
investigated in these media. As a result, the highest cell growth (OD₆₆₀:
9.26, 4.54 g cdw/L) to date was achieved in 34 g/L sugar containing medium. Similarly, the highest quantity of 5-ALA
(37.44 mM) to date was attained in the same growth culture. In addition to
these significant improvements, at maximum 21.02 mL (0.42 L H₂/L) of
hydrogen was collected from 34 g/L sugar containing medium.
To conclude, using a sugar concentration of 34 g/L yielded the highest
bacterial growth and 5-ALA generation so far. And, it also supported the
generation of considerable amount of hydrogen.          

Keywords

5-aminolevulinic acid, hydrogen, molasses, Rhodobacter sphaeroides

Supporting Institution

Selçuk Üniversitesi

Project Number

BAP-11401112

Thanks

The study was supported by Selçuk University with the project number BAP-11401112.

Yüksek Miktarlarda Şeker Pancarı Melasının Hidrojen ve 5-Aminolevulinik Asit Üretimi için Substrat Olarak Değerlendirilmesi

Abstract

Şeker pancarı melası
değerli bir hammaddedir ve sükroz başta olmak üzere yüksek miktarda şeker
içerir. Bu nedenle, mikroorganizmalar tarafından son derece değerli
kimyasalların üretimi için substrat olarak kullanılabilir. Bu çalışmada, Rhodobacter sphaeroides O.U.001'in çoğalması
ile hidrojen ve 5-aminolevulinik asit (5-ALA) üretimlerini arttırıp
arttırmadıklarını araştırmak için ilk kez oldukça yüksek melas konsantrasyonları
test edilmiştir. İlk önce, şeker içeriği 34 g/L, 41 g/L, 48 g/L, 55 g/L ve 61
g/L olan beş farklı ortam, melas kullanılarak hazırlandı. Daha sonra, kesikli
süreçlerle, bu ortamlarda bakteri çoğalması ile hidrojen ve 5-ALA üretimleri
incelenmiştir. Sonuç olarak, bugüne kadarki en yüksek hücre çoğalması (OD660:
9.26, 4.54 g cdw/L), 34 g/L şeker içeren ortamda elde edildi. Benzer şekilde, aynı
çoğalma kültüründe bugüne kadarki en yüksek miktarda 5-ALA (37.44 mM) elde
edildi. Bu önemli gelişmelere ek olarak, 34 g/L şeker içeren ortamdan maksimum
21.02 mL (0.42 L H₂/L) hidrojen toplanmıştır. Sonuç olarak, 34 g/L'lik
bir şeker konsantrasyonunun kullanılması, şimdiye kadarki en yüksek bakteri
üremesini ve 5-ALA oluşumunu sağladı. Ayrıca, önemli miktarda hidrojen üretimini
de destekledi. 

Keywords

5-aminolevulinik asit, hidrojen, melas, Rhodobacter sphaeroides

Project Number

BAP-11401112

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