Influence of Different Concentrations of Murashige and Skoog Medium on Multiple Shoot Regeneration of Staurogyne repens (Nees) Kuntze

Abstract

Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental plants, medicinal and aromatic plants. Therefore, optimization of production techniques is very important. In this study, the effects of different Murashige and Skoog (MS) salt levels (25%-200%) on the in vitro production of Staurogyne repens (Nees) Kuntze were investigated. The shoot tip was used as explant. Different levels of MS salts significantly affected the regeneration abilities of shoot tip explants. The highest shoot regeneration rate was determined at 100% and 150% MS salt levels. The maximum regenerated shoots (9.13 shoots/explant) was determined in nutrient media supplemented with 150% MS salts. The minimum count of shoots (5.22 shoots/explant) was recorded in food media including 200% MS salts. Mean shoot lengths were between 1.65-2.27 cm. The highest length value (2.27 cm) was determined in the culture medium with 100% MS salts added, while the lowest length value (1.65 cm) was determined in the nutrient medium containing 25% MS salts. The shoots in the propagation medium were rooted in nutrient medium supplemented with 0.25 mg/L indole-3-acetic acid (IAA). Then they were successfully acclimatized to external conditions.

Keywords

• Culture medium
• in vitro propagation
• shoot regeneration
• tissue culture

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