Influence of Different Concentrations of Murashige and Skoog Medium on Multiple Shoot Regeneration of Staurogyne repens (Nees) Kuntze

Year 2022, , 61 - 67, 30.04.2022

Muhammet Doğan

https://doi.org/10.30931/jetas.1055833

Abstract

Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental plants, medicinal and aromatic plants. Therefore, optimization of production techniques is very important. In this study, the effects of different Murashige and Skoog (MS) salt levels (25%-200%) on the in vitro production of Staurogyne repens (Nees) Kuntze were investigated. The shoot tip was used as explant. Different levels of MS salts significantly affected the regeneration abilities of shoot tip explants. The highest shoot regeneration rate was determined at 100% and 150% MS salt levels. The maximum regenerated shoots (9.13 shoots/explant) was determined in nutrient media supplemented with 150% MS salts. The minimum count of shoots (5.22 shoots/explant) was recorded in food media including 200% MS salts. Mean shoot lengths were between 1.65-2.27 cm. The highest length value (2.27 cm) was determined in the culture medium with 100% MS salts added, while the lowest length value (1.65 cm) was determined in the nutrient medium containing 25% MS salts. The shoots in the propagation medium were rooted in nutrient medium supplemented with 0.25 mg/L indole-3-acetic acid (IAA). Then they were successfully acclimatized to external conditions.

Keywords

Culture medium, in vitro propagation, shoot regeneration, tissue culture

References

• [1] Phillips, G. C., Garda, M., "Plant tissue culture media and practices: an overview", In Vitro Cellular & Developmental Biology-Plant 55 (2019) : 242-257.
• [2] Bijalwan, P., "Plant tissue culture-A new tool for vegetable improvement (Indian scenario): A review", Agricultural Reviews 42(2) (2021) : 235-239.
• [3] Murashige, T., Skoog, F., "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiologia Plantarum 15 (1962) : 473-497.
• [4] Kumar, A., Chauhan, S., Rattan, S., Warghat, A.R., Kumar, D., Bhargava, B., "In vitro propagation and phyto-chemical assessment of Cymbidium aloifolium (L.) Sw.: An orchid of pharma-horticultural importance", South African Journal of Botany 144 (2022) : 261-269.
• [5] Demétrio, C. A., de Oliveira Jacob, J. F., Ambrosano, G.B., de Oliveira, Ê. T., Rodrigues, P. H. V., "In vitro propagation of cambuci (Campomanesia phaea): An endangered exotic fruit and ornamental plant from Brazilian Atlantic Forest", Plant Cell, Tissue and Organ Culture 145(1) (2021) : 203-208.
• [6] Lin, J., Zou, J., Zhang, B., Que, Q., Zhang, J., Chen, X., Zhou, W., "An efficient in vitro propagation protocol for direct organogenesis from root explants of a multi-purpose plant, Broussonetia papyrifera (L.) L’Hér. ex Vent", Industrial Crops and Products 170 (2021) : 113686.
• [7] Eshghi Khas, M., Abbasifar, A., ValizadehKaji, B., "Optimization of in vitro propagation of purple passion fruit (Passiflora edulis), an important medicinal and ornamental plant", International Journal of Horticultural Science and Technology 7(3) (2020) : 305-314.
• [8] Trang, N. T. Q., Hong, H. T. K., Huong, V. T. M., Long, D. T., "In vitro propagation of red lotus (Nelumbo nucifera Gaertn)-An aquatic edible plant in Vietnam", Agricultural Science Digest 41 (2021) : 129-136.
• [9] Negahdar, N., Hashemabadi, D., Kaviani, B., "In vitro conservation and cryopreservation of Buxus sempervirens L., a critically endangered ornamental shrub", Russian Journal of Plant Physiology 68(4) (2021) : 661-668.
• [10] Razdan, M. K., "Introduction to plant tissue culture", (2003) Science Publishers.
• [11] Kose, M. S. H., Dogan, M., Sadi, G., "Enhanced in vitro shoot proliferation through nodal explants of Staurogyne repens (Nees) Kuntze", Biologia 76(3) (2021) : 1053-1061.
• [12] Kose, M. S. H., Dogan, M., Sadi, G., "Multiple shoot induction and plant regeneration of Staurogyne repens (Nees) Kuntze", Eurasian Journal of Biological and Chemical Sciences 3(2) (2020) : 106-109.
• [13] Sereda, M. M., Lutsenko, E. V., Chokheli, V. A., Vereschagina, A. V., Rachkovskaya, K. Y., Lysenko, V. S., Varduny, T. V., "A method for microclonal propagation of Staurogyne repens in tissue culture". Journal of Plant Sciences 12(1) (2017) : 17-21.
• [14] Fadel, D., Kintzios, S., Economou, A. S., Moschopoulou, G., Constantinidou, H. I. A., "Effect of different strength of medium on organogenesis, phenolic accumulation and antioxidant activity of spearmint (Mentha spicata L.)", The Open Horticulture Journal 3(1) (2010) : 31-35.
• [15] Naik, P. M., Godbole, M., Nagella, P., Murthy, H. N., "Influence of different media, medium strength and carbon sources on adventitious shoot cultures and production of bacoside A in Bacopa monnieri (L.)", Ceylon Journal of Science 46(4) (2017) : 97-104.
• [16] Cortés-Olmos, C., Gurrea-Ysasi, G., Prohens, J., Rodríguez-Burruezo, A., Fita, A., "In vitro germination and growth protocols of the ornamental Lophophora williamsii (Lem.) Coult. as a tool for protecting endangered wild populations", Scientia Horticulturae 237 (2018) : 120-127.
• [17] Ebrahimi, F., "Effect of different hormonal concentrations and culture medium on eglantine (Rosa foetida) propagation in tissue culture medium", Plant Ecophysiology 2 (2010) : 145-149.
• [18] Tetsumura, T., Matsumoto, Y., Sato, M., Honsho, C., Yamashita, K., Komatsu, H., Sugimoto, Y., Kunitake, H., "Evaluation of basal media for micropropagation of four highbush blueberry cultivars", Scientia Horticulturae 119(1) (2008) : 72-74.