Determination of Heavy Metal Accumulation in Milk and Their Packaging Materials and Statistical Analysis

Abstract

Heavy metal accumulation occurs when foods and food contact materials contain excessive amounts of heavy metals. Heavy metal accumulation in foods can risk public health and cause diseases. Therefore, the concentration of heavy metals in food and packaging materials is an important parameter that needs to be analyzed. This study aimed to detect heavy metal accumulation in food and packaging materials. For this purpose, milk, which has an essential place among foods, was chosen. Products of 10 different milk brands used commercially today were supplied. Within the scope of heavy metal analyses, Lead (Pb), Arsenic (As), Cadmium (Cd), Iron (Fe), Manganese (Mn), Zinc (Zn), Copper (Cu), Aluminum (Al), Cobalt (Co) and Nickel (Ni) elements were determined. Pre-treatment of the samples was carried out using the efficient microwave method to ensure minimal sample loss through rapid thawing. Heavy metal analysis was then carried out using the widely used ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometer) device, a widespread technique in current practice. The range of heavy metal concentrations in all packaging samples were: Al (1.219–2.578), As (1.078–1.522), Co (0.219–0.239), Fe (0.862–81.440), Pb (1.784–2.170), Mn (0.042–1.884), Ni (0.782–9.758, Zn (1.814–2.522) mg/kg. Heavy metal concentrations in all packaging samples were found Al (51267.00–71601.25), As (31.626–42.371), Cd (1.035–1.209), Co (0.775–1.167), Cu (4.921–44.839), Fe (259.615–463.182), Pb (24.386–26.668), Mn (4.301–59.599), Ni (6.065–7.943), Zn (5.324–8.763) mg/kg. The heavy metals with the highest concentration among packaging materials are Al and Fe, followed by As and Pb. Additionally, the correlation between milk and its packaging samples were presented using SPSS.

Keywords

• Milk
• food packaging
• microwave
• heavy metal accumulation
• inductively coupled plasma–optical emission spectrometry (ICP-OES)

Supporting Institution

TÜBİTAK

Project Number

TÜBİTAK 2209-A (1919B012215735)

Ethical Statement

Etik beyana gerek yoktur.

Thanks

This work was supported by the TUBITAK 2209-A.

References

1. [1] Yaralı, E., “Gıda Güvenliği” (2018) : 1–132.
2. [2] Ceyhun Sezgin, A., Artık, N., “Toplu Tüketim Yerlerinde Gıda Güvenliği ve HACCP Uygulamaları ( Food Safety and HACCP Applications for Mass Consumption Places )”, Journal of Tourism and Gastronomy Studies 3(2) (2015) : 56–62.
3. [3] Güler, Ü.A., Can, Ö.P., “Araştırma Makalesi Kimyasal Kontaminantların Çevre Sağlığı ve Gıda Güvenliği Üzerine Etkileri”, Sinop Uni J Nat Sci. 195 (2017) : 170–195.
4. [4] Tezcan, N., “Trakya Bölgesinde Üretimi Yapilan Buğday Ve Arpanin Ağir Metal Bulaşanlarinin Tespiti”, Master Thesis (2009).
5. [5] Sonone, S.S., Jadhav, S.V, Sankhla, M.S., Kumar, R., “Water Contamination by Heavy Metals and Their Toxic Effect on Aquaculture and Human Health through Food Chain”, Letters in Applied NanoBioScience 10(2) (2020) : 2148–2166.
6. [6] Alissa, E.M., Ferns, G.A., “Heavy Metal Poisoning and Cardiovascular Disease”, Journal of Toxicology (2011).
7. [7] Cıdıroğlu, Z., Aydın, A., “Investigation of Heavy Metal Residues in Heat-Treated Drinking Milk Offered for Sale in the Market”, Kocatepe Veterinary Journal 14 (2021) : 231–237.
8. [8] Nagpal, R., Behare, P.V., Kumar, M., Mohania, D., Yadav, M., Jain, S., Menon, S., Parkash, O., Marotta, F., Minelli, E., Henry, C.J.K.,Yadav, H., “Milk, Milk Products, and Disease Free Health: An Updated Overview”, Critical Reviews in Food Science and Nutrition 52(4) (2012) : 321–333.

9. [9] Gaucheron, F., “Milk and Dairy Products: A Unique Micronutrient Combination”, Journal of the American College of Nutrition 30 (2011) : 400S-409S.
10. [10] Özturan, K., Atasever, M., “Mineral Elements and Heavy Metals in Milk and Dairy Products”, Ataturk Universitesi Veteriner Bilimleri Dergisi 13(2) (2018) : 229–241.
11. [11] Lante, A., Lomolino, G., Cagnin, M., Spettoli, P., “Content and Characterisation of Minerals in Milk and in Crescenza and Squacquerone Italian Fresh Cheeses by ICP-OES”, Food Control 17(3) (2006) : 229–233.
12. [12] Karasakal, A., “Determination of Trace and Major Elements in Vegan Milk and Oils by ICP-OES After Microwave Digestion”, Biological Trace Element Research 197(2) (2020) : 683–693.
13. [13] Hameed, K.G.A., El-Zamkan, M.A., “Determination of Some Heavy Metals in Flavored Milk by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) and Their Public Health Importance”, World Journal of Dairy & Food Sciences 10(2) (2015) : 193–198.
14. [14] Elafify, M., EL-Toukhy, M., Sallam, K.I., Sadoma, N.M., Mohammed Abd-Elghany, S., Abdelkhalek, A., El-Baz, A.H., “Heavy Metal Residues in Milk and Some Dairy Products with Insight into Their Health Risk Assessment and the Role of Lactobacillus Rhamnosus in Reducing the Lead and Cadmium Load in Cheese”, Food Chemistry Advances 2 (2023) : 100261.
15. [15] Eti, S.A., Islam, M.S., Shourove, J.H., Saha, B., Ray, S.K., Sultana, S., Ali Shaikh, M. A., Rahman, M.M., “Assessment of Heavy Metals Migrated from Food Contact Plastic Packaging: Bangladesh Perspective”, Heliyon 9(9) (2023) : e19667.
16. [16] Bakircioglu, D., Kurtulus, Y.B., Ucar, G., “Determination of Some Traces Metal Levels in Cheese Samples Packaged in Plastic and Tin Containers by ICP-OES after Dry, Wet and Microwave Digestion”, Food and Chemical Toxicology 49(1) (2011) : 202–207.
17. [17] Alamri, M.S., Qasem, A.A.A., Mohamed, A.A., Hussain, S., Ibraheem, M.A., Shamlan, G., Alqah, H.A., Qasha, A.S., “Food Packaging’s Materials: A Food Safety Perspective”, Saudi Journal of Biological Sciences 28(8) (2021) : 4490–4499.
18. [18] Omeroğlu, P.Y., Zdal, T.Ö., Bulut, R., “Chemical Migration from Plastic Types of Food Contact Materials”, Eurasian Journal of Food Science and Technology (2012) : 22–32.
19. [19] Barukčić, I., Ščetar, M., Lisak Jakopović, K., Kurek, M., Božanić, R., Galić, K., “Overview of Packaging Materials for Dairy Packaging”, Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam 16(3–4) (2021) : 85–93.
20. [20] Benyathiar, P., Mishra, D.K., Szemplenski, T.E., David, J.R.D., “Aseptic Filling and Packaging for Retail Products and Food Service”, In Handbook of aseptic processing and packaging CRC Press (2022) : 171-210.
21. [21] Pascall, M.A., Sablani, S.S., Greenwood, P., “Aseptic Packaging”, Encyclopedia of Food Safety, Elsevier (2024) : 664–672.
22. [22] Bhunia, K., Sablani, S.S., Tang, J., Rasco, B., “Migration of Chemical Compounds from Packaging Polymers during Microwave, Conventional Heat Treatment, and Storage”, Comprehensive Reviews in Food Science and Food Safety 12(5) (2013) : 523–545.
23. [23] Güven, D.E., Akinci, G., “Comparison of Acid Digestion Techniques to Determine Heavy Metals in Sediment and Soil Samples”, Gazi University Journal of Science 24(1) (2011) : 29–34.
24. [24] El Hosry, L., Sok, N., Richa, R., Al Mashtoub, L., Cayot, P., Bou-Maroun, E., “Sample Preparation and Analytical Techniques in the Determination of Trace Elements in Food: A Review”, Foods 12(4) (2023).
25. [25] Kasar, S., Murugan, R., Arae, H., Aono, T., Sahoo, S.K., “A Microwave Digestion Technique for the Analysis of Rare Earth Elements, Thorium and Uranium in Geochemical Certified Reference Materials and Soils by Inductively Coupled Plasma Mass Spectrometry”, Molecules 25(21) (2020).
26. [26] Korn, M. das G.A., Morte, E.S. da B., dos Santos, D.C.M.B., Castro, J.T., Barbosa, J.T. P., Teixeira, A.P., Fernandes, A.P., Welz, B., dos Santos, W.P.C., dos Santos, E.B.G.N., Korn, M., “Sample Preparation for the Determination of Metals in Food Samples Using Spectroanalytical Methods - A Review”, Applied Spectroscopy Reviews 43(2) (2008) : 67–92.
27. [27] Douvris, C., Vaughan, T., Bussan, D., Bartzas, G., Thomas, R., “How ICP-OES Changed the Face of Trace Element Analysis: Review of the Global Application Landscape”, Science of The Total Environment 905 (2023) : 167242.
28. [28] Fassel, V.A., Knfseley, R.N., “Inductively Coupled Plasma: Optical Emission Spectroscopy”, Analytical Chemistry 46(13) (1974) : 1110A-1120a.
29. [29] Manousi, N., Isaakidou, E., Zachariadis, G.A., “An Inductively Coupled Plasma Optical Emission Spectrometric Method for the Determination of Toxic and Nutrient Metals in Spices after Pressure-Assisted Digestion”, Applied Sciences 12(2) 82022) : 534.
30. [30] Bulska, E., Wagner, B., “Quantitative Aspects of Inductively Coupled Plasma Mass Spectrometry”, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 374(2079) (2016) : 20150369.
31. [31] Cortez-Trejo, M.C., Manríquez, J., Mendoza, S., “Characterization of Natural Gums: Emphasizing Distinctive Spectroscopic Techniques”, Natural Gums, Elsevier (2023) : 123–161.
32. [32] Obermaier, K., Schmelzeisen-Redeker, G., Schoemaker, M., Klötzer, H.M., Kirchsteiger, H., Eikmeier, H., Del Re, L., “Performance Evaluations of Continuous Glucose Monitoring Systems: Precision Absolute Relative Deviation Is Part of the Assessment”, Journal of Diabetes Science and Technology 7(4) (2013) : 824–832.
33. [33] Peto, M.V., “Aluminium and Iron in Humans: Bioaccumulation, Pathology, and Removal”, Rejuvenation Research 13(5) (2010) : 589–598.
34. [34] Goyer, R.A., Clarkson, T.W., Ag, S., “Clinical Trials of Antihistaminc Drugs in the Prevention and Treatment of the Common Cold: Report by a Special Committee of the Medical Research Council Large-Scale Therapeutic Field Trial”, British Medical Journal 2(4676) (1950) : 425–429.
35. [35] Klotz, K., Weistenhöfer, W., Neff, F., Hartwig, A., van Thriel, C., and Drexler, H., “The Health Effects of Aluminum Exposure”, Deutsches Ärzteblatt international (2017).
36. [36] Stahl, T., Taschan, H., Brunn, H., “Aluminium Content of Selected Foods and Food Products”, Environmental Sciences Europe 23(1) (2011) : 37.
37. [37] Amer, A., Makarem, H., Maghraby, M., Alella, S., “Lead, Cadmium, and Aluminum in Raw Bovine Milk: Residue Level, Estimated Intake, and Fate during Artisanal Dairy Manufacture”, Journal of Advanced Veterinary and Animal Research 8(3) (2021) : 454.
38. [38] Pezeshki, H., Hashemi, M., Rajabi, S., “Removal of Arsenic as a Potentially Toxic Element from Drinking Water by Filtration: A Mini Review of Nanofiltration and Reverse Osmosis Techniques”, Heliyon 9(3) (2023) : 4246.
39. [39] Simsek, O., Gültekin, R., Öksüz, O., Kurultay, S., “The Effect of Environmental Pollution on the Heavy Metal Content of Raw Milk”, Nahrung/Food 44(5) (2000) : 360-363.
40. [40] Suhani, I., Sahab, S., Srivastava, V., Singh, R.P., “Impact of Cadmium Pollution on Food Safety and Human Health”, Current Opinion in Toxicology 27 (2021) : 1–7.
41. [41] Godt, J., Scheidig, F., Grosse-Siestrup, C., Esche, V., Brandenburg, P., Reich, A., Groneberg, D.A., “The Toxicity of Cadmium and Resulting Hazards for Human Health”, Journal of Occupational Medicine and Toxicology 1(1) (2006) : 22.
42. [42] Ahmed, M., Khaleeq, A., Huma, R., Qadir, M.A., Shafiq, M.I., Israr, A., Ali, A., Shahzad, S., “Optimization and Validation Procedure for Elemental Composition of Fresh and Pasteurized Milk in Pakistan Employing Microwave Digestion Followed by ICP-OES: A Contribution to Risk Assessment”, Food Analytical Methods 9(10) (2016) : 2933–2942.
43. [43] Yu, M., Liu, Y., Achal, V., Fu, Q.L., Li, L., “Health Risk Assessment of Al and Heavy Metals in Milk Products for Different Age Groups in China”, Polish Journal of Environmental Studies 24(6) (2015) : 2707–2714.
44. [44] JECFA, “Joint FAO/ WHO Expert Committee on Food Additives Eighty-Seventh Meeting”, (June) (2019) : 1–15.
45. [45] Bost, M., Houdart, S., Oberli, M., Kalonji, E., Huneau, J.-F., Margaritis, I., “Dietary Copper and Human Health: Current Evidence and Unresolved Issues”, Journal of Trace Elements in Medicine and Biology 35 (2016) : 107–115.
46. [46] Bakircioglu, D., Topraksever, N., Yurtsever, S., Kizildere, M., Kurtulus, Y.B., “Investigation of Macro, Micro and Toxic Element Concentrations of Milk and Fermented Milks Products by Using an Inductively Coupled Plasma Optical Emission Spectrometer, to Improve Food Safety in Turkey”, Microchemical Journal 136 (2018): 133–138.
47. [47] Karasakal, A., “Determination of Trace and Major Elements in Vegan Milk and Oils by ICP-OES After Microwave Digestion”, Biological Trace Element Research 197(2) (2020) : 683–693.
48. [48] İnci, A., Ünübol Aypak, S., Güven, G., “Aydin İlinde Üretilen İnek Sütlerinde Bazi Ağir Metal Düzeylerinin Araştirilmasi”, Gida / The Journal Of Food (2017) : 229–234.
49. [49] Lim, K., Riddell, L., Nowson, C., Booth, A., Szymlek-Gay, E., “Iron and Zinc Nutrition in the Economically-Developed World: A Review”, Nutrients 5(8) (2013) : 3184–3211.
50. [50] Turnlund, J., Smith, R., Kretsch, M., Keyes, W., Shah, A., “Milk’s Effect on the Bioavailability of Iron from Cereal-Based Diets in Young Women by Use of in Vitro and in Vivo Methods”, The American Journal of Clinical Nutrition 52(2) (1990) : 373–378.
51. [51] Ghazi, A.M., Millette, J.R., “Lead”, Environmental Forensics, Elsevier (1964) : 55–79.
52. [52] FAO/WHO Expert Committee on Food Additives, “Evaluation of Certain Food Additives and Contaminants: Eightieth Report”, WHO Technical Report Series (2016) : 995.
53. [53] Turkish Food Codex, “Bulaşanlar Yönetmeliği” (2011)
54. [54] Lucchini, R.G., Aschner, M., Yangho kim, Šarić, M., “Manganese”, Handbook on the Toxicology of Metals, Elsevier (2015) : 975–1011.
55. [55] OMS, U., Protection, E., Agency, E. P., Pharmacology, A., “Manganese in Drinking-Water Manganese in Drinking-Water”, 158(December) (2021) : 68.
56. [56] Santos, D.C.M.B., Carvalho, L.S.B., Lima, D.C., Leão, D.J., Teixeira, L.S.G., Korn, M. G.A., “Determination of Micronutrient Minerals in Coconut Milk by ICP OES after Ultrasound-Assisted Extraction Procedure,” Journal of Food Composition and Analysis 34(1) (2014) : 75–80.
57. [57] Genchi, G., Carocci, A., Lauria, G., Sinicropi, M. S., Catalano, A., “Nickel: Human Health and Environmental Toxicology”, International Journal of Environmental Research and Public Health 17(3) (2020) : 679.
58. [58] Schrenk, D., Bignami, M., Bodin, L., Chipman, J. K., del Mazo, J., Grasl‐Kraupp, B., Hogstrand, C., Hoogenboom, L. (Ron), Leblanc, J., Nebbia, C. S., Ntzani, E., Petersen, A., Sand, S., Schwerdtle, T., Vleminckx, C., Wallace, H., Guérin, T., Massanyi, P., Van Loveren, H., Baert, K., Gergelova, P., Nielsen, E., “Update of the Risk Assessment of Nickel in Food and Drinking Water”, EFSA Journal 18(11) (2020).
59. [59] Sharma, P., Reddy, P. K., Kumar, B., “Trace Element Zinc, a Nature’s Gift to Fight Unprecedented Global Pandemic COVID-19”, Biological Trace Element Research 199(9) (2021) : 3213–3221.
60. [60] FAO/WHO, “Joint FAO/WHO Food Standards Programme Codex Committee on Contaminants in Foods. CF/14 INF/1. Fourteenth Session”, CODEX Alimentarius Commission (March) (2021) : 1–90.
61. [61] Algan, G, Tekinşen, O.C., Gök, V., “Investigation of Some Heavy Metal Presence in the Milk of Cattle”, Eurasian Journal of Veterinary Sciences 28(3) (2012) : 159–163.