CLINOPODIUM PAMPHYLICUM SUBSP. DAVISII (CONTANDR. & QUÉZEL) GOVAERTS TOPRAK ÜSTÜ KISIMLARININ ANTİOKSİDAN, ENZİM İNHİBİSYON POTANSİYELİ VE FENOLİK PROFİLİNİN İNCELENMESİ

Abstract

Amaç: Tıbbi ve aromatik bitkilerden oluşan Lamiaceae familyası Türkiye'de 46 cins ve 571 tür ile temsil edilmektedir. Calamintha Miller türlerinin geleneksel kullanımları arasında baharat olarak, uyarıcı, spazm giderici, terletici, balgam söktürücü etkileri ve bunun yanısıra mide ve boğaz ağrılarının tedavisi yer alır. C. pamphylica subsp. davisii (Contandr. & Quézel) P.H. Davis Clinopodium pamphylicum subsp. davisii (Contandr. & Quézel) Govaerts bitkisinin sinonim ismi kabul edilmiştir. C. pamphylicum subsp. davisii Türkiye'ye endemik bir türdür. Şimdiye kadar, bu bitki üzerinde uçucu yağ araştırmaları dışında herhangi bir biyolojik ve fitokimyasal araştırma bildirilmemiştir. C. pamphylicum subsp. davisii’nin antioksidan ve enzim inhibisyonu etkilerinin incelenmesi ile birlikte fitokimyasal bileşenlerinin değerlendirilmesi bu çalışmanın amaçlarıdır.

Gereç ve Yöntem: C. pamphylicum subsp. davisii’nin in vitro antioksidan potansiyelini (DPPH, ABTS, demir şelasyon, Toplam fenolik madde ve flavonoit miktarı) ve asetilkolinesteraz, butirilkolinesteraz, α-glukozidaz, α-amilaz ve tirozinaz gibi enzim inhibitör aktiviteleri spektrofotometrik yöntemler kullanılarak ölçülmüştür. Bitki ekstrelerinin fenolik bileşimini HPLC kullanarak analiz etmek için birçok fenolik asit ve flavonoitin miktarları hesaplanmıştır.

Sonuç ve Tartışma: Sonuçlarımız demir şelasyon dışındaki DPPH ve ABTS yöntemleri ile metanol ekstresinin sulu ekstreden daha yüksek antioksidan özellik sergilediğini göstermiştir. Ayrıca, metanol ekstresi, test edilen asetilkolinesteraz ve α-glukozidaz dışındaki enzimler üzerinde sulu ekstreden daha fazla inhibisyon sergilemiştir. HPLC sonuçlarına gelince, klorojenik asit methanol ekstresi için ana fenolik bileşik olarak belirlenirken, kafeik asit su ekstresinde başlıca fenolik olarak belirlenmiştir. Bu sonuçlar, Türkiye'de halk arasında geleneksel kullanımları olan C. pamphylica subsp. davisii için bilimsel bir temel oluşturmaktadır.

Keywords

• Antioksidan aktivite
• clinopodium pamphylicum
• enzim inhibisyon aktivite
• fenolik bileşikler

EXAMINATION OF THE AERIAL PARTS OF CLINOPODIUM PAMPHYLICUM SUBSP. DAVISII (CONTANDR. & QUÉZEL) GOVAERTS IN TERMS OF ANTIOXIDANT AND ENZYME INHIBITION POTENTIALS, TOGETHER WITH PHENOLIC PROFILE

Abstract

Objective: The family Lamiaceae, consists of medicinal and aromatic plants, is represented by 46 genera and 571 species in Turkey. Traditional uses for Calamintha Miller species include utilizing as a spice, stimulant, antispasmodic, diaphoretic, expectorant, as well as treatment for stomach and throat aches. C. pamphylica subsp. davisii (Contandr. & Quézel) P.H. Davis is accepted as synonym of Clinopodium pamphylicum subsp. davisii (Contandr. & Quézel) Govaerts. C. pamphylicum subsp. davisii is an endemic species to Turkey. Until now, no biological and phytochemical research has been reported on this plant except for its essential oil research. Examining the antioxidant, and enzyme inhibition effects of C. pamphylicum subsp. davisii, as well as determining its phytochemical composition were the targets of the current study.

Material and Method: To evaluate in vitro antioxidant potential (DPPH, ABTS, iron chelating, total phenol and flavonoid amounts) and enzyme inhibitory activities, such as acetylcholinesterase, butyrylcholinesterase, α-glucosidase, α-amylase and tyrosinase for C. pamphylicum subsp. davisii were measured with spectrophotometric methods. The quantities of different phenolic acids and flavonoids were measured using HPLC to analyze the phenolic composition of the plant extracts.

Result and Discussion: Our results indicated that except for iron chelating methanol extract exhibited higher antioxidant properties over water extract using DPPH, and ABTS methods. In addition, methanol extract displayed more inhibition on the tested enzymes except for acetylcholinesterase, and α-glucosidase than water extract. As for HPLC findings, although chlorogenic acid was signified as the main phenolic compound for the methanol extract, caffeic acid was specified as the major phenolic for the water extract. These results provide a scientific basis for C. pamphylica subsp. davisii, which possessed the traditional uses in Turkish folk medicine.

Keywords

• Antioxidant activity
• clinopodium pamphylicum
• enzyme inhibition activity
• phenolic compounds

Thanks

Authors want to put on record their gratitude to the Professor Yavuz Bagcı, Department of Pharmaceutical Botany, Selcuk University, for providing Lab facilities. We would also like to show our gratitude to the Assoc. Prof. Hamide Filiz Ayyıldız for her technical assistant for HPLC analysis. Authors also acknowledges the financial assistance provided by Selcuk University Scientific Research Program.

References

1. 1. Dhanalakshmi, M., Thangadurai, S.A. (2021). Antioxidant and anticancer activities of whole plant extracts of Lepidagathis pungens nees: In vitro evaluation. Pharmacognosy Magazine, 17(5), 63. [CrossRef]
2. 2. Bhandari, M.R., Jong-Anurakkun, N., Hong, G., Kawabata, J. (2008). α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.). Food Chemistry, 106(1), 247-252. [CrossRef]
3. 3. Ayepola, O.R., Brooks, N.L., Oguntibeju, O.O. (2014). Oxidative stress and diabetic complications: the role of antioxidant vitamins and flavonoids. Antioxidant-Antidiabetic Agents and Human Health, 923-931. [CrossRef]
4. 4. Fujiwara, M., Yagi, N., Miyazawa, M. (2010). Acetylcholinesterase inhibitory activity of volatile oil from Peltophorum dasyrachis Kurz ex Bakar (yellow batai) and Bisabolane-type sesquiterpenoids. Journal Agricultural and Food Chemistry. 58(5), 2824-2829. [CrossRef]
5. 5. Jiménez, J., O’Connell, S., Lyons, H., Bradley, B., Hall, M. (2010). Antioxidant, antimicrobial, and tyrosinase inhibition activities of acetone extract of Ascophyllum nodosum. Chemical Papers, 64(4), 434-442. [CrossRef]
6. 6. Pieroni, A., Quave, C., Villanelli, M. L., Mangino, P., Sabbatini, G., Santini, L., Boccetti, T., Profili, M., Ciccioli, T., Rampa, L.G., Antonini, G., Girolamini, C., Cecchi, M., Tomasi, M. (2004). Ethnopharmacognostic survey on the natural ingredients used in folk cosmetics, cosmeceuticals and remedies for healing skin diseases in the inland Marches, Central-Eastern Italy. Journal of Ethnopharmacology, 91(2-3), 331-344. [CrossRef]
7. 7. Sawant, R. L., Lanke, P. D., Wadekar, J. B. (2013). Tyrosinase inhibitory activity, 3D QSAR, and molecular docking study of 2, 5-disubstituted-1, 3, 4-oxadiazoles. Journal of Chemistry, 849782. [CrossRef]
8. 8. Güner, A., Özhatay, N., Ekim, T., Başer, K.H.C. (2000). In Flora of Turkey and the East Aegean Islands (Supplement 2) (Vol. 11). University Press.

9. 9. Alan, S., Kürükçüoğlu, M., Başer, K.H.C. (2011). Composition of Essential Oils of Calamintha nepeta (L.) Savi Subsp. nepeta and Calamintha nepeta (L.) Savi Subsp. glandulosa (Req.) P.W. Ball. Asian Journal of Chemistry, 23(6), 2357-2360.
10. 10. Alan, S., Kürkçüoğlu, M., Başer, K.H.C. (2011). The composition of the essential oils of Calamintha pamphylica subspecies. Turkish Journal of Biology, 35(2), 259-265. [CrossRef]
11. 11. Baser, K. H. C., Kirimer, N. (2006). Essential Oils of Lamiaceae Plants of Turkey I International Symposium on the Labiatae: Advances in Production, Biotechnology and Utilisation, Italy. [CrossRef]
12. 12. Baser, K.H.C., Özek, T., Kürkçüoglu, M., Tümen, G., Duman, H. (1997). Essential Oils of Calamintha pamphylica Boiss. et Heldr. subsp. Pamphylica and subsp. davisii (Quezel et Contandr.) Davis. Journal of Essential Oil Research, 9(3), 371-373. [CrossRef]
13. 13. Alan, S., Ocak, A., Duman, H. (2007). Calamintha pamphylica subsp. alanyense (Lamiaceae), a new subspecies from South Anatolia, Turkey. Annales Botanici Fennici, 44(4), 309-314.
14. 14. Güner, A., Aslan, S. (2012). Türkiye bitkileri listesi:(damarlı bitkiler). Nezahat Gökyiǧit Botanik Bahçesi Yayınları.
15. 15. Göktürk, R.S. (2015). Phaselis antik kenti florası I. Journal of Interdisciplinary Mediterranean Studies, 1.
16. 16. Clarke, G., Ting, K.N., Wiart, C., Fry, J. (2013). High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants, 2(1), 1-10. [CrossRef]
17. 17. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26(9-10), 1231-1237. [CrossRef]
18. 18. Chai, T., Mohan, M., Ong, H., Wong, F. (2014). Antioxidant, iron-chelating and anti-glucosidase activities of Typha domingensis Pers (Typhaceae). Tropical Journal of Pharmaceutical Research, 13(1), 67-72. [CrossRef]
19. 19. Ellman, G. L., Courtney, K. D., Andres Jr, V., Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95. [CrossRef]
20. 20. Jeong, S.H., Ryu, Y.B., Curtis-Long, M.J., Ryu, H.W., Baek, Y.S., Kang, J.E., Lee, W.S., Park, K.H. (2009). Tyrosinase inhibitory polyphenols from roots of Morus lhou. Journal of Agricultural and Food Chemistry, 57(4), 1195-1203. [CrossRef]
21. 21. Lordan, S., Smyth, T.J., Soler-Vila, A., Stanton, C., Ross, R.P. (2013). The α-amylase and α-glucosidase inhibitory effects of Irish seaweed extracts. Food Chemistry, 141(3), 2170-2176. [CrossRef]
22. 22. Özek, G. (2018). Chemical diversity and biological potential of Tanacetum praeteritum subsp. praeteritum essential oils. Journal of the Turkish Chemical Society Section A: Chemistry, 5(2), 493-510. [CrossRef]
23. 23. Gonçalves, S., Moreira, E., Grosso, C., Andrade, P.B., Valentão, P., Romano, A. (2017). Phenolic profile, antioxidant activity and enzyme inhibitory activities of extracts from aromatic plants used in Mediterranean diet. Journal of Food Science and Technology, 54(1), 219-227. [CrossRef]
24. 24. Araniti, F., Lupini, A., Mercati, F., Statti, G.A., Abenavoli, M.R. (2013). Calamintha nepeta L.(Savi) as source of phytotoxic compounds: bio-guided fractionation in identifying biological active molecules. Acta Physiologiae Plantarum, 35(6), 1979-1988. [CrossRef]
25. 25. Pacifico, S., Galasso, S., Piccolella, S., Kretschmer, N., Pan, S.-P., Marciano, S., Bauer, R., Monaco, P. (2015). Seasonal variation in phenolic composition and antioxidant and anti-inflammatory activities of Calamintha nepeta (L.) Savi. Food Research International, 69, 121-132. [CrossRef]
26. 26. Kuntal, D., Raman, D., Gokul Sivaraman, R.P.E. (2018). Phytochemical screening for various secondary metabolites, antioxidant, and anthelmintic activity of Coscinium fenestratum fruit pulp: A new biosource for novel drug discovery. Turkish Journal of Pharmaceutical Sciences, 15(2), 156-165. [CrossRef]
27. 27. Khan, S., Khan, T., Shah, A.J. (2018). Total phenolic and flavonoid contents and antihypertensive effect of the crude extract and fractions of Calamintha vulgaris. Phytomedicine, 47, 174-183. [CrossRef]
28. 28. Conforti, F., Marrelli, M., Statti, G., Menichini, F., Uzunov, D., Solimene, U., Menichini, F. (2012). Comparative chemical composition and antioxidant activity of Calamintha nepeta (L.) Savi subsp. glandulosa (Req.) Nyman and Calamintha grandiflora (L.) Moench (Labiatae). Natural Product Research, 26(1), 91-97. [CrossRef]
29. 29. Ćavar, S., Vidic, D., Maksimović, M. (2013). Volatile constituents, phenolic compounds, and antioxidant activity of Calamintha glandulosa (Req.) Bentham. Journal of the Science of Food and Agriculture, 93(7), 1758-1764. [CrossRef]
30. 30. Popović-Djordjević, J., Cengiz, M., Ozer, M. S., Sarikurkcu, C. (2019). Calamintha incana: essential oil composition and biological activity. Industrial Crops and Products, 128, 162-166. [CrossRef]
31. 31. Vladimir-Knežević, S., Blažeković, B., Kindl, M., Vladić, J., Lower-Nedza, A.D., Brantner, A.H. (2014). Acetylcholinesterase inhibitory, antioxidant and phytochemical properties of selected medicinal plants of the Lamiaceae family. Molecules, 19(1), 767-782. [CrossRef]
32. 32. Arantes, S., Piçarra, A., Candeias, F., Teixeira, D., Caldeira, A.T., Martins, M.R. (2017). Antioxidant activity and cholinesterase inhibition studies of four flavouring herbs from Alentejo. Natural Product Research, 31(18), 2183-2187. [CrossRef]