FARKLI HASTANELERDEN İZOLE EDİLEN ACİNETOBACTER BAUMANNİİ SUŞLARININ GENETİK ÇEŞİTLİLİĞİ

Year 2023, , 104 - 110, 20.01.2023

Hilal Basak Erol , Banu Kaşkatepe , Nurten Altanlar , Sulhiye Yıldız , Süleyman Yalçın

https://doi.org/10.33483/jfpau.1168215

Abstract

Amaç: Suşların genotipik analizi, suşlar arasındaki genetik ilişkileri ve epidemiyolojik araştırmaları değerlendirmek için bilgi sağlayabilir ve farklı coğrafi bölgelerdeki dolaşımlarının izlenmesi için önemlidir. Bu çalışma, dört farklı hastaneden elde edilen klinik Acinetobacter baumannii (A. baumannii) izolatları arasındaki genetik benzerlik veya farklılıkları belirlemeyi amaçlamıştır.

Gereç ve Yöntem: Bu çalışmada, yoğun bakım ünitelerindeki hastalardan 78 A. baumannii klinik izolatı alınmıştır. Dört farklı hastaneden rastgele seçilen ve antibiyotik duyarlılığı bilinen 24 A. baumannii suşunun kolistin MIK değeri belirlenmiştir. Bu suşlar, Enterobacterial repetitive intergenic consensus (ERIC)-PCR yöntemi ile genetik olarak karakterize edilmiştir.

Sonuç ve Tartışma: Çalışmanın sonucunda, izolatların 2 kümeye (A1 ve A2) ayrıldığı ve Küme A2'nin tek bir genotip (C1) ile temsil edildiği ve birbiriyle ilişkili 23 genotipin Küme A1'de olduğunu gösterilmiştir.

Keywords

Acinetobacter baumannii, direnç, ERIC-PCR, genetik çeşitlilik

Supporting Institution

Ankara Üniversitesi

Project Number

18L0237009

GENETIC DIVERSITY OF ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM DIFFERENT HOSPITALS

Abstract

Objective: The genotypic analysis of the strains can provide information to evaluate the genetic relationships among strains and epidemiological investigations, and it is crucial for monitoring their circulation in different geographic regions. This study was to aim to identify genetic similarities or dissimilarities among clinical Acinetobacter baumannii (A. baumannii) isolates from four different hospitals.

Material and Method: In this study, 78 non-duplicate clinical isolates of A. baumannii were received from patients in the critical care units. The colistin MIC values of 24 A. baumannii strains randomly selected from four different hospitals and known to have antibiotic susceptibility were determined. These strains were genetically characterized by the Enterobacterial repetitive intergenic consensus (ERIC)-PCR method.

Result and Discussion: The results of the study showed that the isolates were divided into 2 clusters (A1 and A2) and Cluster A2 was represented by a single genotype (C1) and 23 interrelated genotypes were in Cluster A1.

Keywords

Acinetobacter baumannii, ERIC-PCR, genetic diversity, resistance

Project Number

18L0237009

References

• 1. Vincent, J.L., Rello, J., Marshall, J., Silva, E., Anzueto, A., Martin, C.D., Reinhart, K. (2009). International study of the prevalence and outcomes of infection in intensive care units. Jama, 302(21), 2323-2329. [CrossRef]
• 2. Roca, I., Espinal, P., Vila-Farrés, X., Vila, J. (2012). The Acinetobacter baumannii Oxymoron: Commensal Hospital Dweller Turned Pan-Drug-Resistant Menace. Frontiers in Microbiology, 3, 148-169. [CrossRef]
• 3. Kiyasu, Y., Hitomi, S., Funayama, Y., Saito, K., Ishikawa, H. (2020). Characteristics of invasive Acinetobacter infection: A multicenter investigation with molecular identification of causative organisms. Journal of Infection and Chemotherapy, 26(5), 475-482. [CrossRef]
• 4. Lima, W.G., Alves, M.C., Cruz, W.S., Paiva, M.C. (2018). Chromosomally encoded and plasmid-mediated polymyxins resistance in Acinetobacter baumannii: a huge public health threat. European Journal of Clinical Microbiology and Infectious Diseases, 37(6), 1009-1019. [CrossRef]
• 5. An, J.H., Kim, Y.H., Moon, J.E., Jeong, J.H., Kim, S.H., Kang, S.J., Jang, H.C. (2017). Active surveillance for carbapenem-resistant Acinetobacter baumannii in a medical intensive care unit: Can it predict and reduce subsequent infections and the use of colistin?. American Journal of Infection Control, 45(6), 667-672. [CrossRef]
• 6. Antunes, L.C., Visca, P., Towner, K.J. (2014). Acinetobacter baumannii: evolution of a global pathogen. Pathogens and Disease, 71(3), 292-301. [CrossRef]
• 7. Maleki, A., Vandyousefi, J., Mirzaie, Z., Ghafourian, S., Kazemian, H., Sadeghifard, N. (2017). Molecular Analysis of the Isolates of Acinetobacter baumannii isolated from Tehran Hospitals Using ERIC-PCR Method. Modern Medical Laboratory Journal, 1(1), 12-16. [CrossRef]
• 8. Aljindan, R., Alsamman, K., Elhadi, N. (2018). ERIC-PCR genotyping of Acinetobacter baumannii isolated from different clinical specimens. Saudi Journal of Medicine and Medical Sciences, 6(1), 13-17. [CrossRef]
• 9. Testing EUCAST (2016). EUCAST disk diffusion test manual, version 4.0. [CrossRef]
• 10. European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 10.0, valid from 2020-01-01. Basel, Switzerland: EUCAST [accessed 15 Mart 2020].
• 11. Dorneles, E.M., Santana, J.A., Ribeiro, D., Dorella, F.A., Guimaraes, A.S., Moawad, M.S., Lage, A.P. (2014). Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates. PLoS One, 9(6), e98758. [CrossRef]
• 12. McConnell, M.J., Actis, L., Pachon, J. (2013). Acinetobacter baumannii: human infections, factors contributing to pathogenesis and animal models. FEMS Microbiol Review, 37(2), 130-155. [CrossRef]
• 13. Fishbain, J., Peleg, A.Y. (2010). Treatment of Acinetobacter infections. Clinical Infectious Diseases, 51(1), 79-84. [CrossRef]
• 14. Karaoglan, I., Zer, Y., Bosnak, V.K., Mete, A.O., Namiduru, M. (2013). In vitro synergistic activity of colistin with tigecycline or beta-lactam antibiotic/beta-lactamase inhibitor combinations against carbapenem-resistant Acinetobacter baumannii. Journal of International Medical Research, 41(6), 1830-1837. [CrossRef]
• 15. Principe, L., D'Arezzo, S., Capone, A., Petrosillo, N., Visca, P. (2009). In vitro activity of tigecycline in combination with various antimicrobials against multidrug resistant Acinetobacter baumannii. Annals of Clinical Microbiology and Antimicrobials, 8, 18-29. [CrossRef]
• 16. Zarifi, E., Ghazalibina, M., Mansouri, S., Morshedi, K., Pourmajed, R., Arfaatabar, M. (2020). Molecular typing of Acinetobacter baumannii clinical strains by enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Gene Reports, 18, 100542. [CrossRef]
• 17. Hazhirkamal, M., Zarei, O., Movahedi, M., Karami, P., Shokoohizadeh, L., Taheri, M. (2021). Molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Hamadan, west of Iran. BMC pharmacology and toxicology, 22(1), 32-32. [CrossRef]
• 18. Falah, F., Shokoohizadeh, L., Adabi, M. (2019). Molecular identification and genotyping of Acinetobacter baumannii isolated from burn patients by PCR and ERIC-PCR. Scars, Burns and Healing, 5, 2059513119831369. [CrossRef]
• 19. Gautam, H., Maheshwari, B., Mohapatra, S., Sood, S., Dhawan, B., Kapil, A., Tezpur, B. (2022). Clonal relationship among Acinetobacter baumannii isolates from different clinical specimens by ERIC-PCR. International Journal of Infectious Diseases, 116, S18-S19. [CrossRef]
• 20. Lotfi, F., Shojaie, M., Rahbarnia, L., Dehnad, A., Naghili, B., Lotfi, H. (2022). Molecular characterization and genetic diversity of multidrug- and extensively drug-resistant A. baumannii clinical isolates. Gene Reports, 26, 101455. [CrossRef]