ENDEMİK ASPERULA LILACIFLORA SUBSP. PHRYGIA (BORNM.) SCHÖNB. -TEM. (RUBIACEAE) ÜZERİNDE FARMASÖTİK BOTANİK ARAŞTIRMA

Abstract

Amaç: Bu çalışmada Rubiaceae familyasına ait endemik Asperula lilaciflora subsp. phrygia’nın farmasötik botanik açıdan araştırılması amaçlanmıştır. Gereç ve Yöntem: Eskişehir-İnönü’den toplanan bitki morfolojik araştırmalarda değerlendirilirken, tüy ve polen morfolojisi SEM’de incelenmiştir. Anatomik çalışmalarda %70’lik alkole alınan örneklerin manuel kesitlerinde, yapıları aydınlatılmış ve fotoğrafları çekilmiştir. Toplam fenolik madde miktarı Folin-Ciocalteu reaktifi ile test edilmiştir. Bitki ekstresinin antioksidan aktivitesi ise DPPH ve ABTS süpürücü etki tayini ile belirlenmiştir. Sonuç ve Tartışma: Bitkinin morfolojik, anatomik, tüy, polen ve kimyasal özellikleri ilk kez bu çalışmayla ortaya koyulmuştur. Morfolojik çalışmada taksonun ayrıntılı deskripsiyonu verilmiş, çizimler ve fotoğraflarla desteklenmiştir. Gövde ve yapraklarda yoğun hispit tüyler tanımlanmıştır. Polenler hekzakolpat olup, eliptik şekillidir. Gövdeler, dört köşeli-yuvarlak, kollateral tipte ve ergastik madde ile rafit kristalleri içermektedir. Yapraklar monofasiyal tiptedir. Toplam fenolik madde miktarı 1 g A. lilaciflora subsp. phrygia ekstresinde 31 mg gallik asite eşdeğer (GAE) olarak hesaplanmıştır. Antioksidan aktivitesi DPPH IC50=0.38 mg/ml, ABTS IC50=0.37 mg/ml-0.90 mg/ml olarak bulunmuştur.

Keywords

• Anatomi
• antioksidan
• Asperula
• morfoloji
• palinoloji

THE PHARMACEUTICAL BOTANICAL INVESTIGATION ON ENDEMIC ASPERULA LILACIFLORA SUBSP. PHRYGIA (BORNM.) SCHÖNB. -TEM. (RUBIACEAE)

Abstract

Objective: This study aimed to investigate the aspects of pharmaceutical botany of endemic Asperula lilaciflora subsp. phrygia in Rubiaceae family. Material and Method: While the plant from Eskişehir-İnönü was studied morphologically, it was also examined by SEM for trichome and pollen morphology. In anatomical studies, the structures of the samples taken in 70% alcohol were illuminated in manual sections and their photographs were taken. The total amount of phenolic substances was tested with the Folin-Ciocaalteu reagent. The antioxidant activity of the plant extract was determined by DPPH and ABTS scavenging effect assays. Result and Discussion: The morphological, anatomical, trichome, pollen, and chemical properties of the plant were revealed for the first time in this study. A detailed description of the taxon was given in the morphological study, supported by drawings and photographs. The stem and leaves are densely hispid hairy. Pollen is hexacolpate and elliptical. The stems are tetragonal-round, collateral type and contain ergastic material, raphide crystals. Leaves are monofacial. The total amount of phenolic substances in 1 g of extract was calculated as 31 mg of gallic acid equivalent (GAE). Antioxidant activity was found to be DPPH IC50= 0.38 mg/ml, ABTS IC50=0.37 mg/ml-0.90 mg/ml.

Keywords

• Anatomy
• antioxidant
• Asperula
• morphology
• palynology

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