ANOGEISSUS LEIOCARPUS'UN SULU VE ETANOLİK EKSTRELERININ FİTOKİMYASAL PROFİLİ VE AĞIR METAL BİLEŞİMİ

Abstract

Amaç: Bu çalışma, A. leiocarpus'un fitokimyasal ve ağır metal bileşenlerinin etnomedikaldeki uygulamalarını göz önünde bulundurmayı amaçlamıştır.

Gereç ve Yöntem: Fitokimyasal bileşenler kalitatif ve kantitatif olarak gravimetrik olarak belirlenirken, bileşen tanımlaması (Gaz spektrometresi-kütle spektrometresi (GC-MS)) tekniği kullanılarak yapılmıştır. Ağır metallerin miktarı atomik absorpsiyon spektrofotometresi ile belirlenmiştir.

Sonuç ve Tartışma: Saponinler ve flavonoidler sulu ekstrelerde sırasıyla %10.22 ±0.48 ve %38.67 ±0.17 konsantrasyonlarında ve etanol ekstraktında sırasıyla %17.37 ±0.65 ve %19.63 ±0.60 konsantrasyonlarında tespit edildi. Ancak alkaloidler, steroidler, glikozitler ve terpenoidler tespit edilmedi. GC-MS analizi, sulu ve etanol özütlerinde sırasıyla 16 ve 26 bileşik tanımladı. Sulu ekstrede 5-Hidroksimetilfurfural, 1,2,4-Benzenetriol ve cis-Vaccenic asit sırasıyla 46.24, 17.12 ve 15.13 ile en yüksek pik alanlarına sahipken, etanol ekstresinde 5-Hidroksimetilfurfural (%14.40), 1,2,3-Benzenetriol (%12,29) ve -metoksibenzen-1,4-diol (%7,54) en yüksek değerlerdi. Krom (0,548 ppm ±0,030) sadece sulu konsantrasyonda tespit edilirken Kadmiyum, sulu ve etanol özütünde sırasıyla 0,002 ±0,001 ve 0,006 ppm ±0,002 konsantrasyona sahipti. Kurşun, sırasıyla 0.096 ± 0.020 ve 0.096 ppm ± 0.040'lık sulu ve etanol özütlerinde mevcuttu. Sonuç olarak, bu çalışma, bitkinin farklı rahatsızlıkları tedavi etmek için folklorik tıpta geleneksel uygulamasına ilişkin iddialarla hemfikirdir.

Keywords

• Ağır metaller
• Anogeissus leiocarpus
• GC-MS
• fitokimyasal
• profil oluşturma

PHYTOCHEMICAL PROFILING AND HEAVY METALS COMPOSITION OF AQUEOUS AND ETHANOL EXTRACTS OF ANOGEISSUS LEIOCARPUS

Abstract

Objective: The present study aimed to investigate the phytochemical and heavy metals components of A. leiocarpus considering its applications in ethnomedicine.

Material and Method: The phytochemical components were determined qualitatively and quantitatively gravimetrically, while component identification was done using (Gas spectrometer-mass spectrometer (GC-MS) technique. Heavy metals were quantified by atomic absorption spectrophotometer.

Result and Discussion: Saponins and flavonoids were detected in the aqueous extracts in concentrations of 10.22% ±0.48, and 38.67% ±0.17 respectively, and concentrations of 17.37% ±0.65 and 19.63% ±0.60 respectively in the ethanol extract. GC-MS analysis identified 16 and 26 compounds in the aqueous and ethanol extracts respectively. In the aqueous extract, 5-Hydroxymethylfurfural, 1,2,4-Benzenetriol, and cis-Vaccenic acid had the highest peak areas of 46.24, 17.12, and 15.13% respectively, while in the ethanol extract 5-Hydroxymethylfurfural (14.40%), 1,2,3-Benzenetriol (12.29%) and -methoxybenzene-1,4-diol (7.54%) were the highest. Chromium (0.548 ppm ±0.030) was detected only in the aqueous concentration, while Cadmium had a concentration of 0.002 ±0.001 and 0.006 ppm ±0.002 in the aqueous and ethanol extract respectively. Lead was present with aqueous and ethanol extracts concentrations of 0.096 ±0.020 and 0.096 ppm ±0.040 respectively. Conclusively, the present study agrees with the claims for the traditional application of the plant in folkloric medicine to manage different ailments.

Keywords

• Anogeissus leiocarpus
• GC-MS
• heavy metals
• phytochemicals
• profiling

References

1. 1. Singh, R. (2015). Medicinal plants: A review. Journal of Plant Sciences, 3(1-1), 50-55. [CrossRef]
2. 2. Hassan, B.A.R. (2012). Medicinal plants (importance and uses). Pharmaceutica Analytica Acta, 3(10), 1000-1139. [CrossRef]
3. 3. Kuruppu, A.I., Paranagama, P., Goonasekara, C.L. (2019). Medicinal plants commonly used against cancer in traditional medicine formulae in Sri Lanka. Saudi Pharmaceutical Journal, 27(4), 565-573. [CrossRef]
4. 4. Panmei, R., Gajurel, P.R., Singh, B. (2019). Ethnobotany of medicinal plants used by the Zeliangrong ethnic group of Manipur, northeast India. Journal of Ethnopharmacology, 235, 164-182. [CrossRef]
5. 5. Atanasov, A.G., Waltenberger, B., Pferschy-Wenzig, E.M., Linder, T., Wawrosch, C., Uhrin, P., Temml, V., Wang, L., Schwaiger, S., Heiss, E.H., Rollinger, J.M., Schuster, D., Breuss, J.M., Bochkov, V., Mihovilovic, M.D., Kopp, B., Bauer, R., Dirsch, V.M., Stuppner, H. (2015). Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology Advances, 33(8), 1582-1614. [CrossRef]
6. 6. Prasathkumar, M., Anisha, S., Dhrisya, C., Becky, R., Sadhasivam, S. (2021). Therapeutic and pharmacolo-gical efficacy of selective Indian medicinal plants - A review. Phytomedicine Plus, 1(2), 100029. [CrossRef]
7. 7. Mukhtar, Y., Abdu, K., Maigari, A.K. (2017). Efficacy of Anogeissus leiocarpus (DC.) as potential therapeutic agent against Trypanosomiasis diseases: A review. International Journal of Health and Pharmaceutical Research, 3, 1-9.
8. 8. Singh, D., Baghel, U.S., Gautam, A., Baghel, D.S., Yadav, D., Malik, J., Yadav, R. (2016). The genus Anogeissus: A review on ethnopharmacology, phytochemistry and pharmacology. Journal of Ethnopharmacology, 194, 30-56. [CrossRef]

9. 9. Adrien, K.M., Calixte, B., Honoré, T., Lucien, B.G., Vincent, E., Joseph, D.A., David, N.J. (2015). Antifungal Activity of Roots Barks Extract of Securinega virosa (Roxb. ex Willd.) Baill and Anogeissus leiocarpa (DC.) Guill. & Perr, Two Plants Used in the Traditional Treatment of Candidiasis in Northern Côte d'Ivoire. International Journal of Clinical Biochemistry and Research, 8(11), 1-11.
10. 10. Muhammad, H.A., Yusha’u, M., Taura, D.W., Aliyu, A.M. (2022). Antibacterial activity and phytochemical constituents of African birch (Anogeissus leiocarpus) stem bark extracts. Bayero Journal of Pure and Applied Sciences, 13(1), 447-454.
11. 11. Orlando, G., Ferrante, C., Zengin, G., Sinan, K.I., Bene, K., Diuzheva, A., Jekő, J., Cziáky, Z., Di Simone, S., Recinella, L. and Chiavaroli, A. (2019). Qualitative chemical characterization and multidirectional biological ınvestigation of leaves and bark extracts of anogeissus leiocarpus (DC.) Guill. & Perr. (Combretaceae). Antioxidants, 8(9), 343. [CrossRef]
12. 12. Tauheed, A.M., Mamman, M., Ahmed, A., Suleiman, M.M., Balogun, E.O. (2020). In vitro and in vivo antitrypanosomal efficacy of combination therapy of Anogeissus leiocarpus, Khaya senegalensis and potash. Journal of Ethnopharmacology, 258, 112805. [CrossRef]
13. 13. Motto, A.E., Lawson-Evi, P., Eklu-Gadegbeku, K. (2022). Antidiabetic and antioxidant potential of total extract and supernatant fraction of the roots of Anogeissus leiocarpus in HFD-fed and streptozocin -induced diabetic rats. Biomedicine & Pharmacotherapy, 154, 113578. [CrossRef]
14. 14. Evans, W.C. (2009). Trease and Evans' pharmacognosy: Elsevier Health Sciences.
15. 15. Obadoni, B., Ochuko P. (2002). Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Sciences, 8(2), 203-208. [CrossRef]
16. 16. Harborne, A. (1998). Phytochemical methods a guide to modern techniques of plant analysis: springer science & business media.
17. 17. AOAC. (1998). Official methods of analysis. 16 ed. USA: Association of Official Analytical Chemists. 18. Sharma, S., Kumari, A., Dhatwalia, J., Guleria, I., Lal, S., Upadhyay, N., Kumar, V., Kumar, A. (2021). Effect of solvents extraction on phytochemical profile and biological activities of two Ocimum species: A comparative study. Journal of Applied Research on Medicinal and Aromatic Plants, 25, 100348. [CrossRef]
18. 19. Biswas, T., Dwivedi, U.N. (2019). Plant triterpenoid saponins: biosynthesis, in vitro production, and pharmacological relevance. Protoplasma, 256(6), 1463-1486. [CrossRef]
19. 20. Zhao, Y.-Z., Zhang, Y.-Y., Han, H., Fan, R.-P., Hu, Y., Zhong, L., Kou, J.P., Yu, B.Y. (2018). Advances in the antitumor activities and mechanisms of action of steroidal saponins. Chinese Journal of Natural Medicines, 16(10), 732-748. [CrossRef]
20. 21. Wang, T., Choi, R.C.Y., Li, J., Bi, C.W.C., Ran, W., Chen, X., Dong, T.T., Bi, K., Tsim, K.W. (2012). Trillin, a steroidal saponin isolated from the rhizomes of Dioscorea nipponica, exerts protective effects against hyperlipidemia and oxidative stress. Journal of Ethnopharmacology, 139(1), 214-220. [CrossRef]
21. 22. Yang, L., Ren, S., Xu, F., Ma, Z., Liu, X., Wang, L. (2019). Recent advances in the pharmacological activities of dioscin. BioMed Research International, 2019, 5763602. [CrossRef]
22. 23. Zheng, Y., Chen, L., Liu, Y., Shi, L., Wan, S., Wang, L. (2019). Evaluation of antimicrobial activity of water-soluble flavonoids extract from Vaccinium bracteatum Thunb. leaves. Food Science and Biotechnology, 28(6), 1853-1859. [CrossRef]
23. 24. Hu, Y.W., Liu, C.Y., Du, C.M., Zhang, J., Wu, W.Q., Gu, Z.L. (2009). Induction of apoptosis in human hepatocarcinoma SMMC-7721 cells in vitro by flavonoids from Astragalus complanatus. Journal of Ethnopharmacology, 123(2), 293-301. [CrossRef]
24. 25. Ahmad, A., Kaleem, M., Ahmed, Z., Shafiq H. (2015). Therapeutic potential of flavonoids and their mechanism of action against microbial and viral infections—A review. Food Research International, 77, 221-235. [CrossRef]
25. 26. Ciumărnean, L., Milaciu, M.V., Runcan, O., Vesa, Ș.C., Răchișan, A.L., Negrean, V., Perné, M.G., Donca, V.I., Alexescu, T.G., Para, I., Dogaru, G. (2020). The effects of flavonoids in cardiovascular diseases. Molecules, 25(18), 4320. [CrossRef]
26. 27. Ezeonu, C.S., Ejikeme, C.M. (2016). Qualitative and quantitative determination of phytochemical contents of indigenous Nigerian softwoods. New Journal of Science, 5601327. [CrossRef]
27. 28. Alhassan, D.A., Uba, A.I., Muhammad, A.U., Muhammad, Y.Y.u. (2016). Phytochemical screening and antimicrobial activity of crude stem bark extracts of Anogeissus leiocarpus. European Journal of Medicinal Plant, 11(2), 1-7. [CrossRef]
28. 29. Timothy, S.Y., Mashi, F.I., Helga, B.I., Galadima, I.H., Midala, T.A.S. (2015). Phytochemical screening, antibacterial evaluation and in vitro spasmodic effect of the aqueous and ethanol leaf and bark extract of Anogeissus leiocarpus (DC) Guill. & Perr. Asian Journal of Pharmaceutical science & Technology, 5(4), 302-208.
29. 30. Namadina, M.M., Mukhtar, A.U., Karaye, S.I., Musa, F.M., Bah, I.H., Maitama, F.Y. (2021). Phytochemical constituents and antibacterial activities of indigenous chewing stick (Anogeissus leiocarpus) stem. Bayero Journal of Pure and Applied Sciences, 14(1), 85-94. [CrossRef]
30. 31. Zhao, L., Chen, J., Su, J., Lin, L., Hu, S.-Q., Li, B., Zhang, X., Xu, Z., Chen, T. (2013). In vitro antioxidant and antiproliferative activities of 5-hydroxymethylfurfural. Journal of Agricultural and Food Chemistry, 61(44), 10604-10611. [CrossRef]
31. 32. Zhang, H., Jiang, Z., Shen, C., Zou, H., Zhang, Z., Wang, K., Bai, R., Kang, Y., Ye, X.Y., Xie, T. (2021). 5-hydroxymethylfurfural alleviates ınflammatory lung ınjury by ınhibiting endoplasmic reticulum stress and nlrp3 ınflammasome activation. Frontiers in Cell and Developmental Biology, 9, 2021- 2029. [CrossRef]
32. 33. Hamazaki, K., Suzuki, N., Kitamura, K.-i., Hattori, A., Nagasawa T., Itomura, M., Hamazaki, T. (2016). Is vaccenic acid (18: 1t n-7) associated with an increased incidence of hip fracture? An explanation for the calcium paradox. Prostaglandins, Leukotrienes and Essential Fatty Acids, 109, 8-12. [CrossRef]
33. 34. Cynthia, F.I., Hery, S., Akhmad, D. (2018). Antibacterial and antioxidant activities of pyrogallol and synthetic pyrogallol dimer. Research Journal of Chemistry and Environment, 22, 39-47.
34. 35. Oliveira, L.C.C., Rodrigues, F.A.A., dos Santos Barbosa, C.R., dos Santos, J.F.S., Macêdo, N.S., de Sousa Silveira, Z., Coutinho, H.D.M.; da Cunha, F.A.B (2022). Antibacterial activity of the pyrogallol against Staphylococcus aureus evaluated by optical ımage. Biologics, 2(2), 139-150. [CrossRef]
35. 36. Hamed, A., Mantawy, E., El-Bakly, W., Abdel-Mottaleb, Y., Azab S. (2020). Methyl palmitate: the naturally occurring cardioprotective agent. Archives of Pharmaceutical Sciences Ain Shams University, 4(1), 47-62. [CrossRef]
36. 37. Carrillo, Pérez C., Cavia, Camarero, M.D.M., Alonso de la Torre, S. (2012). Role of oleic acid in immune system; mechanism of action; a review. Nutrición Hospitalaria, 27(4), 978-990. [CrossRef]
37. 38. Muzalevskaya, E.N., Miroshnichenko, L.A., Nikolaevskii, V.A., Ushakov, I.B., Chernov, Y.N., Alabovskii, V.V., Batishcheva, G.A., Buzlama, A.V. (2015). Squalene: Physiological and pharmacological properties, Eksperimental'naia I Klinicheskaia Farmakologiia, 78(6), 30-36.
38. 39. Costa, M. (2019). Review of arsenic toxicity, speciation and polyadenylation of canonical histones. Toxicology and Applied Pharmacology, 375, 1-4. [CrossRef]
39. 40. Tchounwou, P.B., Yedjou, C.G., Patlolla, A.K., Sutton, D.J. (2012). Heavy metal toxicity and the environment. Experientia Supplementum, 101, 133-164. [CrossRef]
40. 41. Pavesi, T., Moreira, J.C. (2020). Mechanisms and individuality in chromium toxicity in humans. Journal of Applied Toxicology, 40(9), 1183-1197. [CrossRef]
41. 42. Lech, T., Sadlik, J.K. (2017). Cadmium concentration in human autopsy tissues. Biological Trace Element Research, 179(2), 172-177. [CrossRef]
42. 43. Djordjevic, V.R., Wallace, D.R., Schweitzer, A., Boricic, N., Knezevic, D., Matic, S., Grubor, N., Kerkez, M., Radenkovic, D., Bulat, Z., Antonijevic, B., Matovic, V., Buha, A. (2019). Environmental cadmium exposure and pancreatic cancer: Evidence from case control, animal and in vitro studies. Environment International, 128, 353-361. [CrossRef]
43. 44. Kianoush, S., Balali-Mood, M., Mousavi, S.R., Moradi, V., Sadeghi, M., Dadpour, B., Rajabi, O., Shakeri, M.T. (2012). Comparison of therapeutic effects of garlic and d‐Penicillamine in patients with chronic occupational lead poisoning. Basic & Clinical Pharmacology & Toxicology, 110(5), 476-481. [CrossRef]
44. 45. World Health O. (1996). Permissible limits of heavy metals in soil and plants. Geneva, Switzerland, from https://scholar.google.com/scholar_lookup?title=Permissible%20limits%20of%20heavy%20metals%20in%20soil%20and%20plants&pages=45-67&publication_year=1996. Accessed date: 14.01.2023.
45. 46. Ouédraogo, S., Sombié, B.C., Ouédraogo, J.C.W., Nitiéma, M., Belemnaba, L., Ouédraogo, S., Semdé, R. (2017). Quality control of trunk’s barks of Lannea microcarpa Engl. and K. Krause and Anogeissus leiocarpus (DC) Guill. & Perr. for the manufacture of phytomedicines for the treatment of hypertension. International Journal of Phytopharmacy, 7(4), 36-41. [CrossRef]
46. 47. Mustapha, B.A., Kubmarawa, D., Shagal, M.H., Hayatudeen, A. (2015). Heavy metal profiles of medicinal plants found within the vicinity of quarry site in Demsa, Adamawa State, Nigeria. British Journal of Applied Science & Technology, 13(1), 1-6. [CrossRef]
47. 48. Uba, A., Tsafe, A.I., Achor, M., Baburo, S.I.B. (2016). Determination of physico-chemical parameters and some heavy metals in selected herbal drugs sold in kara market, sokoto- nigeria. FUW Trends in Science & Technology Journal, 1(2), 558-562.
48. 49. Algül, F., Beyhan, M. (2020). Concentrations and sources of heavy metals in shallow sediments in Lake Bafa, Turkey. Science Report 10, 11782. [CrossRef]
49. 50. Mazhari, S.A., Attar, R.S., Haghighi, F. (2017). Heavy metals concentration and availability of different soils in Sabzevar area, NE of Iran. Journal of African Earth Sciences, 134, 106-112. [CrossRef]
50. 51. Li, H., Shi, A., Li, M., Zhang, X. (2013). Effect of pH, temperature, dissolved oxygen, and flow rate of overlying water on heavy metals release from storm sewer sediments. Journal of Chemistry, 2013, 434012. [CrossRef]