ANOGEISSUS LEIOCARPUS'UN SULU VE ETANOLİK EKSTRELERININ FİTOKİMYASAL PROFİLİ VE AĞIR METAL BİLEŞİMİ

Year 2023, , 311 - 323, 20.05.2023

Muhammad Mubarak Dahıru , Enoch Buba Badgal , Neksumi Musa

https://doi.org/10.33483/jfpau.1205941

Cited By: 4

Abstract

Amaç: Bu çalışma, A. leiocarpus'un fitokimyasal ve ağır metal bileşenlerinin etnomedikaldeki uygulamalarını göz önünde bulundurmayı amaçlamıştır.

Gereç ve Yöntem: Fitokimyasal bileşenler kalitatif ve kantitatif olarak gravimetrik olarak belirlenirken, bileşen tanımlaması (Gaz spektrometresi-kütle spektrometresi (GC-MS)) tekniği kullanılarak yapılmıştır. Ağır metallerin miktarı atomik absorpsiyon spektrofotometresi ile belirlenmiştir.

Sonuç ve Tartışma: Saponinler ve flavonoidler sulu ekstrelerde sırasıyla %10.22 ±0.48 ve %38.67 ±0.17 konsantrasyonlarında ve etanol ekstraktında sırasıyla %17.37 ±0.65 ve %19.63 ±0.60 konsantrasyonlarında tespit edildi. Ancak alkaloidler, steroidler, glikozitler ve terpenoidler tespit edilmedi. GC-MS analizi, sulu ve etanol özütlerinde sırasıyla 16 ve 26 bileşik tanımladı. Sulu ekstrede 5-Hidroksimetilfurfural, 1,2,4-Benzenetriol ve cis-Vaccenic asit sırasıyla 46.24, 17.12 ve 15.13 ile en yüksek pik alanlarına sahipken, etanol ekstresinde 5-Hidroksimetilfurfural (%14.40), 1,2,3-Benzenetriol (%12,29) ve -metoksibenzen-1,4-diol (%7,54) en yüksek değerlerdi. Krom (0,548 ppm ±0,030) sadece sulu konsantrasyonda tespit edilirken Kadmiyum, sulu ve etanol özütünde sırasıyla 0,002 ±0,001 ve 0,006 ppm ±0,002 konsantrasyona sahipti. Kurşun, sırasıyla 0.096 ± 0.020 ve 0.096 ppm ± 0.040'lık sulu ve etanol özütlerinde mevcuttu. Sonuç olarak, bu çalışma, bitkinin farklı rahatsızlıkları tedavi etmek için folklorik tıpta geleneksel uygulamasına ilişkin iddialarla hemfikirdir.

Keywords

Ağır metaller, Anogeissus leiocarpus, GC-MS, fitokimyasal, profil oluşturma

PHYTOCHEMICAL PROFILING AND HEAVY METALS COMPOSITION OF AQUEOUS AND ETHANOL EXTRACTS OF ANOGEISSUS LEIOCARPUS

Abstract

Objective: The present study aimed to investigate the phytochemical and heavy metals components of A. leiocarpus considering its applications in ethnomedicine.

Material and Method: The phytochemical components were determined qualitatively and quantitatively gravimetrically, while component identification was done using (Gas spectrometer-mass spectrometer (GC-MS) technique. Heavy metals were quantified by atomic absorption spectrophotometer.

Result and Discussion: Saponins and flavonoids were detected in the aqueous extracts in concentrations of 10.22% ±0.48, and 38.67% ±0.17 respectively, and concentrations of 17.37% ±0.65 and 19.63% ±0.60 respectively in the ethanol extract. GC-MS analysis identified 16 and 26 compounds in the aqueous and ethanol extracts respectively. In the aqueous extract, 5-Hydroxymethylfurfural, 1,2,4-Benzenetriol, and cis-Vaccenic acid had the highest peak areas of 46.24, 17.12, and 15.13% respectively, while in the ethanol extract 5-Hydroxymethylfurfural (14.40%), 1,2,3-Benzenetriol (12.29%) and -methoxybenzene-1,4-diol (7.54%) were the highest. Chromium (0.548 ppm ±0.030) was detected only in the aqueous concentration, while Cadmium had a concentration of 0.002 ±0.001 and 0.006 ppm ±0.002 in the aqueous and ethanol extract respectively. Lead was present with aqueous and ethanol extracts concentrations of 0.096 ±0.020 and 0.096 ppm ±0.040 respectively. Conclusively, the present study agrees with the claims for the traditional application of the plant in folkloric medicine to manage different ailments.

Keywords

Anogeissus leiocarpus, GC-MS, heavy metals, phytochemicals, profiling

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