KULLANILMIŞ RUJ VE MASKARALARDA MİKROBİYOLOJİK KONTAMİNASYONUNUN ARAŞTIRILMASI

Abstract

Amaç: Kozmetik ürünlerin içeriğinde yer alan karbon ve azot kaynakları, mineraller, büyüme faktörleri ve asidik ortam, nem gibi uygun ortam koşulları mikrobiyal üreme için elverişli koşulların oluşmasını sağlar. Bu ürünlerin mikrobiyolojik kontaminasyonu hem tüketici sağlığı açısından risk oluşturması hem de üründe meydana gelebilecek değişiklikler nedeniyle ekonomik kayıplara neden olması açısından önem taşımaktadır. Bu çalışmada tüketiciler tarafından kullanılmış ruj ve rimel örneklerinin mikrobiyolojik kontaminasyon açısından incelenmesi amaçlanmıştır.

Gereç ve Yöntem: Çalışmada tüketiciler tarafından kullanılmış otuz adet ruj ve otuz adet rimel örneği mikrobiyolojik kontaminasyon açısından incelenmiştir. Numuneler, Avrupa Standardı EN ISO 17516'da belirtilen mikrobiyolojik limit değerlerine göre değerlendirilmiştir. Kontaminant bakteriler VITEK® 2 Compact (bioMérieux, Fransa) otomatize sistemi ile tanımlanmıştır.

Sonuç ve Tartışma: Elde edilen sonuçlara göre, test edilen maskara örneklerinden yalnızca birinin, ruj örneklerinden ise dördünün belirtilen mikrobiyolojik limit değerlerine uygun olmadığı saptanmıştır. Maskara örneğinden izole edilen bakteri Staphylococcus sciuri, ruj örneklerinden izole edilen bakteriler ise Kocuria kristinae, Streptococcus mitis, Streptococcus oralis, Streptococcus alactolyticus, Rothia mucilaginosa, Rothia dentocariosa türleri olarak tanımlanmıştır. Kontaminasyona neden olan bakterilerin çoğunluğunun ağız, boğaz, solunum ve cilt mikrobiyotasının üyeleri, Gram pozitif türler olduğu görülmüştür. Test edilen kozmetik ürünlerin bulaşının, tüketicilerin uygun olmayan kullanımlarından, aynı ürünü birden fazla kişinin kullanmasından, tükürük bulaşından, el hijyenine dikkat edilmemesinden kaynaklanmış olabileceği düşünülmüştür. 

INVESTIGATION OF THE MICROBIOLOGICAL CONTAMINATION OF USED LIPSTICKS AND MASCARAS

Abstract

Objective: Carbon and nitrogen sources, minerals, growth factors in the composition of cosmetic products, and environmental conditions such as convenient acidic environment and humidity constitute the suitable environment for microbial growth. Microbiological contamination of these products is important in terms of both posing a risk to consumer health and causing economic losses due to changes occurring in the product. This study aimed to examine the lipstick and mascara samples used by consumers in terms of microbiological contamination.

Material and Method: Thirty lipsticks and thirty mascaras used by consumers were investigated for microbiological contamination. The samples were evaluated according to the microbiological limits specified in the European Standard EN ISO 17516. Contaminant bacteria were identified by VITEK® 2 Compact (bioMérieux, France).

Result and Discussion: According to the results obtained, it was determined that only one of the tested mascara samples and four of the lipstick samples did not comply with the specified microbiological limit values. Staphylococcus sciuri was the contaminant bacterium determined in the mascara sample. In addition, Kocuria kristinae, Streptococcus mitis, Streptococcus oralis, Streptococcus alactolyticus, Rothia mucilaginosa, Rothia dentocariosa were detected in lipstick samples. All the bacteria detected as contaminants are Gram-positive, most of which are the members of the mouth, throat, respiratory, and skin microbiota. The contamination may have been caused by the inappropriate use of consumers, such as using the same product by more than one person, contamination of saliva, and not paying attention to hand hygiene.

Keywords

• Cosmetics
• EN ISO 17516
• microbial contamination
• microbiological quality control
• safety testing

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