SANGUISORBA MINOR SUBSP. BALEARICA’NIN FREUND’S COMPLETE ADJUVAN İLE OLUŞTURULMUŞ ARTRİTLİ RAT MODELİNDE ANTİ-ARTRİTİK AKTİVİTESİ

Year 2023, , 576 - 587, 20.05.2023

Aysun İnan Genç , Ayşe Mine Gençler Özkan , Orhan Adalı

https://doi.org/10.33483/jfpau.1254518

Abstract

Amaç: Sanguisorba L. cinsine ait bitkilerin tedavi edici özellikleri pek çok in vivo ve in vitro çalışma ile araştırılmış olup; anti-viral, anti-ülserojenik, anti-kanser, anti-asetilkolinesteraz, radyasyon koruyucu, anti-alerjik ve anti-enflamatuvar etkileri gösterilmiştir. Çalışmamız, Sanguisorba L. cinsine ait bir alt tür olan “Sanguisorba minor subsp. balearica (Bourg. ex Nyman) Muñoz Garm. & C.Navarro” (Smb) bitkisinin artrit modeli oluşturulmuş ratlarda anti-artritik etkisini araştırmayı amaçlamaktadır.
Gereç ve Yöntem: Smb’nin toprak üstü kısımlarının ekstresi birbirini takip eden aşamalardan oluşan maserasyon yöntemi ile elde edilmiştir. Ekstrenin fitokimyasal içeriği Yüksek Performanslı Sıvı Kromatografisi (YPSK) metodu kullanılarak tayin edilmiştir. Anti-artritik etkinliğin araştırılması için Freund’s Complete Adjuvan (CFA) ile indüklenerek oluşturulmuş artritli ratlar kullanılmıştır.
Sonuç ve Tartışma: YPSK analiz sonuçları Smb ekstresinin yüksek miktardaki fenolik içeriğini ortaya koymuştur. Tanımlanan fenolikler şu şekildedir; elajik asit , gallik asit, kuversetin dehidrat ve p-kumarik asittir ve tespit edilen miktarları sırasıyla 4.1288;2.6342; 0.0871 and 0.0633 ppm.’dir. Bitki ekstresi uygulanması CFA ile indüklenen artritli ratların ayak bölgelerindeki enflamasyonda bir azalmaya sebep olmuştur. Enflamasyon miktarındaki en yüksek baskılanma kuversetin ve Smb 70 mg/kg uygulanan grupta tespit edilmiştir ve bu etki diklofenak uygulanan gruptan daha yüksektir. Smb 34 mg/kg uygulanan grupta enflamasyon yine diklofenak uygulanan gruptan daha yüksek oranda baskılanmıştır. Ayak eklemlerinden alınan mikro kesitlere yapılan histopatolojik analizlere göre hem 34 mg. hem de 70 mg. Smb ekstresi artrite bağlı enflamatuvar deformasyonu iyileştirmiştir.

Keywords

Anti-artritik aktivite, artrit, Complete Freund, histopatoloji, Sanguisorba minor subsp. balearica

Supporting Institution

ODTÜ-DPT- Araştırma Destek Programı

Project Number

BAP-08-11-DPT-2011K121010

ANTI-ARTHRITIC ACTIVITY OF SANGUISORBA MINOR SUBSP. BALEARICA AGAINST FREUND’S COMPLETE ADJUVANT-INDUCED ARTHRITIS IN RATS

Abstract

Objective: Therapeutic properties of Sanguisorba L. genus plants are supported by numerous in vivo and in vitro studies showing the anti-viral, anti-ulcerogenic, anti-cancer, anti-acetylcholinesterase, radioprotective, anti-allergic, and anti-inflammatory activities. The present study was designed to investigate anti-arthritic activity of the “Sanguisorba minor subsp. balearica (Bourg. ex Nyman) Muñoz Garm. & C.Navarro” (Smb), a subspecies of Sanguisorba L. genus, on Complete Freund’s Adjuvant induced (CFA-induced) arthritic rat model.
Material and Method: The extract of aerial parts of the Smb was obtained by the consecutive steps of maceration process. The phytochemical content of the extract was analyzed by the High-Performance Liquid Chromatography (HPLC) method. Complete Freund’s Adjuvant-induced arthritic rats were used to examine the anti-arthritic activity of the plant extract.
Result and Discussion: HPLC analysis revealed a high amount of phenolic compounds in the Smb extract. The identified phenolics are ellagic acid, gallic acid, quercetin dehydrate and p-coumaric acid with the concentrations of 4.1288; 2.6342; 0.0871 and 0.0633 ppm, respectively. Injection of Smb extract caused a decrease in paw thickness in the CFA-induced arthritic animals. The decrease in paw thicknesses of animals treated by quercetin and Smb 70 mg/kg groups was higher than diclofenac sodium group. Smb 34 mg/kg group also showed a decrease in paw thickness, but it was lower compared to quercetin and Smb 70 mg/kg groups and higher than the diclofenac sodium group. According to the histopathological evaluations of the joint tissues, both 34 mg/kg and 70 mg/kg Smb extract treatment were improved the inflammatory deformations related to arthritis on the rats.

Keywords

Anti-arthritic activity, arthritis, Complete Freund, histopathology, Sanguisorba minor subsp. balearica

Project Number

BAP-08-11-DPT-2011K121010

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