ENDEMİK THYMUS BRACHYCHILUS JALAS (LAMIACEAE) ÜZERİNDE ANATOMİK, PALİNOLOJİK VE MİKROMORFOLOJİK ARAŞTIRMALAR

Abstract

Amaç: Bu çalışmada, endemik Thymus brachychilus Jalas'ın anatomik ve palinolojik özelliklerinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Anatomik inceleme için bitki materyali %70 alkol içinde fikse edildi. Gövde ve yaprak enine kesitleri, yaprak yüzey kesitleri sartur solüsyonu ile boyandı ve anatomik açıdan incelendi. Yaprak ve gövdenin trikom yapıları, mezofil hücreleri, stoma indeksi, salgı ve örtü tüyleri incelendi, aynı zamanda diğer Thymus türlerinin anatomik bulguları ile karşılaştırıldı. Ayrıca anatomik yapıların ölçümleri yapıldı. SEM'de polen, çiçek kısımları, yaprakların epidermis ve trikom yapısı, kısımları incelenmiştir. Sonuç ve Tartışma: Thymus brachychilus üzerinde yapılan ilk anatomik, palinolojik ve mikromorfolojik çalışmadır. Yaprağın epidermisi tek sıra uzun şekilli hücrelerden oluşur ve ince bir kütikül ile kaplanmıştır. Üst ve alt epidermiste glandüler olmayan ve glandüler trikomlar vardır. İki farklı tipte nonglandüler trikom bulundu. Stomalar diastiktir ve epidermis amfistomatiktir. Mezofil tabakası dorsiventraldir. Gövde enine kesiti karedir. En dıştaki tek sıralı, uzun, kare veya dikdörtgen epidermis ince bir kütikül ile kaplıdır. Polen simetrisi izopolardır ve şekli suboblate. Hexacolpate ve bireticulate özelliklere sahiptir.

Keywords

• Aladağlar
• mikromorfoloji
• palinoloji
• bitki anatomisi
• Thymus brachychilus

Project Number

PB2020-ECZ-1

ANATOMICAL, PALYNOLOGICAL AND MICROMORPHOLOGICAL INVESTIGATIONS ON ENDEMIC THYMUS BRACHYCHILUS JALAS (LAMIACEAE)

Abstract

Objective: In this study, it was aimed to investigate the anatomical and palynological features of endemic Thymus brachychilus Jalas. Material and Method: The plant material was fixed in 70% alcohol for anatomical investigation. Cross sections of the stem, leaves, and leaf surface sections were stained with sartur solution, and the anatomical aspects were examined. The trichome architectures, mesophyll cells, stomatal index, and glandular and non-glandular trichomes features of leaves and stem have been investigated and compared with the other Thymus species anatomical findings. In addition, measurements of anatomical structures were made. Pollen, flower parts, epidermis and trichome structure of the leaves, were examined in SEM. Result and Discussion: This is the first anatomical, palynological and micromorphological study on Thymus brachychilus. The epidermis of leaf consists of a single row of elongated shaped cells and is covered with a thin cuticle. There are nonglandular and glandular trichomes in the upper and lower epidermis. Two different types of nonglandular trichomes were found. Stomata are diacytic, and the epidermis is amphistomatic. The mesophyll layer is dorsiventral. The stem cross-section is square. The outermost single-row, elongated, square or rectangular epidermis is covered with a thin cuticle. The pollen symmetry is isopolar, shape is suboblate. It has hexacolpate and bireticulate properties.

Keywords

• Thymus brachychilus
• plant anatomy
• Aladağlar
• micromorphology
• palynology

Supporting Institution

Altınbaş Üniversitesi

Project Number

PB2020-ECZ-1

Thanks

The authors thank DÜAGEM (Altınbaş University Natural Products Research and Development Center) and BAP-Altınbaş University

References

1. 1. World Flora Online Web site. (2022). From http://www.worldfloraonline.org. Accessed Date: 14.05.2023.
2. 2. Bizim Bitkiler Web site. (2012). From http://www.bizimbitkiler.org.tr. Accessed Date: 14.05.2023.
3. 3. Jalas, J. (1982). Thymus. In: P.H. Davis (Eds.), Flora of Turkey and the East Aegean Islands, vol. 7, (pp. 349-82). Edinburg, University Press.
4. 4. Gurbanov, K., Savran, A. (2018). Kızıltepe (Niğde: Ulukışla) ve çevresinin florası. Bağbahçe Bilim Dergisi, 5, 17-42.
5. 5. Özdemir Nath, E., Kültür, Ş. (2022). An ethnobotanical study of medicinal plants in Savaştepe (Balıkesir-Turkey). Clinical and Experimental Health Sciences, 12(4), 954-980. [CrossRef]
6. 6. Gürdal, B., Kültür, Ş. (2013). An ethnobotanical study of medicinal plants in Marmaris (Muğla, Turkey). Journal of Ethnopharmacology, 146(1), 113-126. [CrossRef]
7. 7. Altundag, E., Ozturk, M. (2011). Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia - Social and Behavioral Sciences, 19, 756-777. [CrossRef]
8. 8. Cakilcioglu, U., Turkoglu, I. (2010). An ethnobotanical survey of medicinal plants in Sivrice (Elazığ-Turkey). Journal of Ethnopharmacology, 132(1), 165-175. [CrossRef]

9. 9. Kaval, I., Behçet, L., Cakilcioglu, U. (2014). Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-Turkey). Journal of Ethnopharmacology, 155, 171-184. [CrossRef]
10. 10. Cakilcioglu, U., Khatun, S., Turkoglu, I., Hayta, S. (2011). Ethnopharmacological survey of medicinal plants in Maden (Elazig-Turkey). Journal of Ethnopharmacology, 137, 469-486. [CrossRef]
11. 11. Polat, R., Cakilcioglu, U., Satıl, F. (2013). Traditional uses of medicinal plants in Solhan (Bingöl-Turkey). Journal of Ethnopharmacology, 148(3), 951-963. [CrossRef]
12. 12. Yeşil, Y., İnal, İ. (2019). Traditional knowledge of wild edible plants in Hasankeyf (Batman Province, Turkey). Acta Societatis Botanicorum Poloniae, 88(3), 3633. [CrossRef]
13. 13. Polat, R., Satıl, F. (2012). An ethnobotanical survey of medicinal plants in Edremit Gulf (Balıkesir-Turkey). Journal of Ethnopharmacology, 139(2), 626-641. [CrossRef]
14. 14. Özdemir, E., Alpınar, K. (2015). An ethnobotanical survey of medicinal plants in western part of central Taurus Mountains: Aladaglar (Nigde-Turkey). Journal of Ethnopharmacology, 166, 53-65. [CrossRef]
15. 15. Fakir, H., Korkmaz, M., Icel B. (2016). Medicinal plants traditionally used for pain alleviation in Antalya Province, Turkey. Studies on Ethno-Medicine, 10(3), 314-324. [CrossRef]
16. 16. Nikolić, M., Glamočlija, J., Ferreira, I.C.F.R., Calhelha, R.C., Fernandes, Â., Marković, T., Marković, D, Giweli, A., Soković, M. (2014). Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Thymus vulgaris L. essential oils. Industrial Crops and Products, 52, 183-190. [CrossRef]
17. 17. Jovanović, A.A., Balanč, B.D., Petrović, P., Pravilović, R., Djordjević, V.B. (2021). Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review. Journal of Engineering and Processing Management, 13(2), 32-41. [CrossRef]
18. 18. Golkar, P., Mosavat, N., Jalali, S.A.H. (2020). Essential oils, chemical constituents, antioxidant, antibacterial and in vitro cytotoxic activity of different Thymus species and Zataria multiflora collected from Iran. South African Journal of Botany, 130, 250-258. [CrossRef]
19. 19. Dash, K.T., Jena, S., Ray, A., Sahoo, A., Kar, S.K., Sahoo, R.K., Subudhi, E., Chandra Panda, P., Nayak, S. (2021). Chemical composition of carvacrol rich leaf essential oil of Thymus vulgaris from India: assessment of antimicrobial, antioxidant and cytotoxic potential. Journal of Essential Oil Bearing Plants, 24(5), 1134-1145. [CrossRef]
20. 20. Al-Shahrani, M.H., Mahfoud, M., Anvarbatcha, R., Athar, M.T., Al Asmari, A. (2017). Evaluation of antifungal activity and cytotoxicity of Thymus vulgaris essential oil. Pharmacognosy Communications, 7(1), 34-40. [CrossRef]
21. 21. Walker, J.W., Doyle, J.A. (1975). The bases of angiosperm phylogeny: Polynology. Annals of the Missouri Botanical Garden, 62, 664-723.
22. 22. Selvi̇, S., Açar, M., Satıl, F. (2008). Comparative micromorphological and anatomical investigations on Thymus pulvinatus and T. cherlerioides (Lamiaceae) growing in Kazdağı (Edremit-Balıkesir/Turkey). Biological Diversity and Conservation, 6, 12-20.
23. 23. Kütükalan, D., Doğan, M. (2019). Master Thesis. A micromorphological study on the genus Thymus Lamiaceae in Turkey. Middle East Technical University, Ankara, Türkiye.
24. 24. Kızılpınar, İ., Özüdoğru, B., Tarıkahya, B., Doğan, C., Erik, S. (2009). Pollen morphology of some Thymus L. (Lamiaceae) taxa used as spice in Anatolia, Mellifera, 18, 13-22.