SECONDARY METABOLITES OF ENDEMIC CENTAUREA APHRODISEA BOISS.

Year 2024, , 1 - 7, 20.01.2024

Fazilet Gürer , Serdar Demir , Şüra Baykan , Canan Karaalp

https://doi.org/10.33483/jfpau.1330413

Abstract

Objective: This study aimed to isolate secondary metabolites from the aerial parts of endemic Centaurea aphrodisea Boiss. using several chromatographic methods and elucidate the structure of the compounds by using spectroscopic methods.
Material and Method: Aerial parts of the endemic C. aphrodisea were collected from Bozdağ (Ödemiş, İzmir) and n-hexane, chloroform and metanol extracts were prepared. The chloroform extract was investigated by using various chromatographic methods, and the structures of the isolated compounds were determined using spectroscopic methods (1D-2D NMR and LC-MS).
Result and Discussion: One elemane type sesquiterpene (methyl 8α,6α,15-trihydroxyelema-1,3,11(13)-trien-12-oate) and four flavone derivatives (cirsimaritin, 3'-O-methyl eupatorin, eupatorin and salvigenin) were isolated and identified. In addition, the presence of a phenylpropanoid glycoside (syringin) was determined in a fraction by comparison with a referance compound using TLC technique. These compounds are reported for the first time from C. aphrodisea with this study.

Keywords

Centaurea aphrodisea, elemane, flavone, secondary metabolite, sesquiterpene

Supporting Institution

This study was supported by the Scientific Research Projects Directorate of Ege University

Project Number

Project No: 15-ECZ-003

Thanks

The authors would like to thank Prof. Serdar Gokhan Senol for identification of the plant, and Dr. Salih Gunnaz for all NMR measurements. This study was supported by the Scientific Research Projects Directorate of Ege University (Project No: 15-ECZ-003).

ENDEMİK CENTAUREA APHRODISEA BOISS.’İN SEKONDER METABOLİTLERİ

Abstract

Amaç: Bu çalışmada endemik Centaurea aphrodisea Boiss. bitkisinin toprak üstü kısımlarında bulunan sekonder metabolitlerin, kromatografik yöntemlerle saflaştırılması ve spektroskopik yöntemlerlede yapılarının aydınlatılması amaçlanmıştır.
Gereç ve Yöntem: Bu çalışmada, endemik C. aphrodisea’nın toprak üstü kısımları Bozdağ’dan (Ödemiş, İzmir) toplanmış, ve n-hekzan, kloroform ve metanol ekstreleri hazırlanmıştır. Kloroform ekstresi çeşitli kromatografik yöntemler kullanılarak incelenmiş ve izole edilen bileşiklerin yapıları spektroskopik yöntemler (1D-2D NMR ve LC-MS) kullanılarak aydınlatılmıştır.
Sonuç ve Tartışma: Bir eleman tip seskiterpen (metil 8α,6α,15-trihidroksielema-1,3,11(13)-trien-12-oat) ve dört flavon türevi (sirsimaritin, 3'-O-metil öpatorin, öpatorin ve salvigenin) izole edilerek yapıları aydınlatılmıştır. Ayrıca İTK tekniği ve şahit bileşikler kullanılarak bir fraksiyonda fenilpropanoit glikozitinin (siringin) varlığı saptanmıştır. Bu bileşikler C. aphrodisea’dan tarafımızca ilk kez rapor edilmektedir.

Keywords

Centaurea aphrodisea, elemane, flavon, sekonder metabolit, seskiterpen

Project Number

Project No: 15-ECZ-003

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