MICROMERIA MYRTIFOLIA BOISS. & HOHEN’İN BİYOLOJİK AKTİVİTELERİNİN BELİRLENMESİ

Abstract

Amaç: Lamiaceae familyası, güçlü tıbbi niteliklere sahip bilinen veya az bilinen değişik bitkileri içeren bir bitki ailesidir. Micromeria Benth cinsi bu familyaya ait, çok çeşitli biyolojik, fitokimyasal ve etnobotanik kullanımı olan, çoğunlukla otsu bitkilerden oluşan cinslerden biridir. Bu çalışmada, Micromeria myrtifolia'nın metanol ve etanol ekstreleri, bitkinin biyolojik aktiviteleri gösterilmek üzere seçilmiştir Gereç ve Yöntem: Antioksidan aktiviteyi göstermek için DPPH radikal süpürücü aktivitesi ve total fenolik içerik deneyleri yapılmıştır. Daha sonra ekstrelerin asetilkolinesteraz (AChE) ve monoamin oksidaz-A (MAO-A) üzerindeki etkileri değerlendirilmiştir. Sonuç ve Tartışma: Çalışma sonunda metanol ekstraktı, 10 mg/ml dozunda % 96.55 değeriyle en yüksek DPPH radikal süpürücü aktivitesi göstermiştir. Uygulanabilecek en yüksek konsantrasyonlarda metanol ve etanol ekstreleri için AChE inhibisyonları sırasıyla %25 ve %27 olarak saptanmıştır. Ayrıca bitkinin MAO-A üzerine etkisi de tespit edilmiştir. Buna göre etanol ekstresi için IC50 değeri 32.5876 ± 0.89 g/ml ve metanol ekstresi için ise 34.6544 ± 0.76 g/ml olarak bulunmuştur. Elde edilen sonuçlara göre M. myrtifolia'nın potansiyel bir antioksidan etkisi gösterebileceği söylenebilir. Daha ileri çalımalar ile Micromeria myrtifolia'nın çeşitli nörolojik hastalıkların tedavisi için doğal bir kaynak olarak kullanılabileceği düşünülmektedir.

Keywords

• Antioksidan aktivite
• asetilkolinesteraz
• Micromeria myrtifolia
• monoamin oksidaz-A
• nörodejeneratif hastalıklar

DETERMINATION OF BIOLOGICAL ACTIVITIES OF MICROMERIA MYRTIFOLIA BOISS. & HOHEN

Abstract

Objective: Lamiaceae family has a wide variety of well-known and lesser-known plants with strong medicinal qualities. The genus Micromeria Benth. is a member of this family consisting of herbaceous plants with a variety of significant biological, phytochemical, and ethnobotanical uses. In this study, the biological activities of methanol and ethanol extracts of Micromeria myrtifolia were evaluated. Material and Method: To demonstrate the antioxidant activity DPPH radical scavenging activity and total phenolic content assays were done. The effects of the extracts on acetylcholinesterase (AChE) and monoamine oxidase-A were then assessed. Result and Discussion: Methanol extract showed the highest DPPH scavenging activity, at the dose of 10 mg/ml with a value of 96.55%. For the highest concentration that can be applicable, AChE inhibitions for the methanol and ethanol extracts were 25% and 27%, respectively. On the other hand, the inhibitory effects of the ethanol and methanol extracts of the plant on MAO-A were determined; for the ethanol extract IC50 value was found as 32.5876 ± 0.89 g/ml, and for the methanol extract it was found as 34.6544 ± 0.76 g/ml. It can be told that M. myrtifolia can act as a potential antioxidant. With further research and investigation, it is thought that Micromeria myrtifolia could be used as a natural source for the treatment of various neurological diseases.

Keywords

• Acetylcholinesterase
• antioxidant activity
• Micromeria myrtifolia
• monoamine oxidase-A
• neurodegenerative diseases

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