INVESTIGATION CORRELATION BETWEEN THE ANTIOXIDANT, ANTIMICROBIAL, ANTI-FUNGI ACTIVITIES AND CONTENT OF PHENOLIC COMPOUNDS OF RASPBERRY LEAF (RUBUS IDAEUS L.) EXTRACTS

Year 2024, , 1142 - 1154, 10.09.2024

Olexander Maslov , Mykola Komisarenko Svitlana Ponomarenko Tetiana Osolodchenko Sergii Kolisnyk

https://doi.org/10.33483/jfpau.1465242

Abstract

Objective: The purpose of work was study the total content of some biologically active substances (BAS), determine antimicrobial, anti-fungi and antioxidant activities of obtained raspberry leaf extracts, and study a correlation analysis between the content of natural compounds and antimicrobial/antifungal and antioxidant activities.
Material and Method: The quantity of phenolic compounds, catechins, flavonoids and hydroxycinnamic acids was determined by spectrophotometric method of analysis, whereas organic acids by alkalimetric method; antioxidant activity of obtained extracts was evaluated by potentiometric method, antimicrobial and anti-fungi wad determined by method of "well".
Result and Discussion: Results demonstrates the highest amount of polyphenols, flavonoids, catechins and hydroxycinnamic acids were 1.85±0.02, 1.01±0.02, 0.33±0.01 and 0.24±0.005% in 60% ethanolic extract, respectively. The organic acids were dominated in aqueous extract (1.02±0.02%). The most potent antioxidant property possessed 60% extract of raspberry leaf. There is a high correlation between the content of polyphenols, catechins and antioxidant activity, in the case of inhibition of S. aureus, P. aeruginosa, B. subtillis depends on polyphenols, catechins and antioxidant activity, whereas fungi C. albicans significantly depends only on the content of hydroxycinnamic acids as well as E. coli is not depend on any BAS. These findings show the great potential in the development and creation of new medicines with antimicrobial, antioxidant and antifungal effects that are not inferior to, and even superior to, the effects of synthetic analogues.

Keywords

Antimicrobial activity, anti-fungi, antioxidant activity, correlation analysis, polyphenols, raspberry leaf

AHUDUDU YAPRAĞI (RUBUS IDAEUS L.) SIVI EKSTRELERİNİN ANTİOKSİDAN, ANTİMİKROBİYAL, ANTİ-FUNGAL AKTİVİTELERİ İLE FENOLİK BİLEŞİKLERİNİN İÇERİĞİ ARASINDAKİ KORELASYONUN İNCELENMESİ

Abstract

Amaç: Çalışmanın amacı, bazı biyolojik olarak aktif maddelerin (BAS) toplam içeriğini incelemek, elde edilen ahududu yaprağı ekstrelerinin antimikrobiyal, anti-fungal ve antioksidan aktivitelerini belirlemek ve doğal bileşiklerin içeriği ile antimikrobiyal/antifungal ve antioksidan aktivitleri arasındaki korelasyon analizini incelemektir.
Gereç ve Yöntem: Fenolik bileşikler, kateşinler, flavonoitler ve hidroksisinamik asitlerin miktarı spektrofotometrik analiz yöntemiyle, organik asitlerin miktarı ise alkalimetrik yöntemle belirlenmiştir. Elde edilen ekstrelerin antioksidan aktiviteleri potansiyometrik yöntemle değerlendirilmiş, antimikrobiyal ve anti-fungal aktivite ise "kuyu" yöntemiyle belirlenmiştir.
Sonuç ve Tartışma: Sonuçlar; en yüksek polifenol, flavonoit, kateşin ve hidroksisinnamik asit miktarının sırasıyla %1.85±0.02, 1.01±0.02, 0.33±0.01 ve %0.24±0.005 olarak %60 etanolik ekstrede olduğunu göstermiştir. Sulu ekstrede organik asitler baskın bulunmuştur (%1.02±0.02). En güçlü antioksidan özelliği %60’lık ahududu yaprağı ekstresi göstermiştir. Polifenol ve kateşin içeriği ile antioksidan etki arasında yüksek korelasyon tespit edilmiştir; S. aureus, P. aeruginosa, B. subtillis'in inhibisyonunun polifenoller ile kateşinlerin içeriğine ve ve antioksidan aktiviteye bağlı olduğu görülürken; C. albicans mantarlarına karşı etkinin yalnızca hidroksisinnamik asit içeriği ile önemli ölçüde ilişkili olduğu tespit edilmiştir. E. coli üzerine etkinin ise herhangi bir BAS'a bağlı olmadığı gözlenmiştir. Bu bulgular; sentetik analogların etkilerinden daha düşük olmayan ve hatta onlardan daha üstün etkinlik göstermesi dolayısıyla, antimikrobiyal, antioksidan ve anti-fungal etkilere sahip yeni ilaçların geliştirilmesi ve yaratılmasındaki büyük potansiyeli göstermektedir.

Keywords

Ahududu yaprağı, antibakteriyel aktivite, anti-fungal, antioksidan aktivite, korelasyon analizi, polifenoller

References

• 1. Ikuta, K.S., Swetschinski, L.R., Robles Aguilar, G., Sharara, F., Mestrovic, T., Gray, A.P., Davis Weaver, N., Wool, E.E., Han, C., Gershberg Hayoon, A., Aali, A., Abate, S.M., Abbasi-Kangevari, M., Abbasi-Kangevari, Z., Abd-Elsalam, S., Abebe, G., Abedi, A., Abhari, A.P., Abidi, H., Naghavi, M. (2022). Global mortality associated with 33 bacterial pathogens in 2019: A systematic analysis for the Global Burden of Disease Study 2019. The Lancet, 400, 2221-2248 [CrossRef]
• 2. Bongomin, F., Gago, S., Oladele, R., Denning, D. (2017). Global and multi-national prevalence of fungal diseases-estimate precision. Journal of Fungi, 3(4), 57. [CrossRef]
• 3. Denning, D. (2022). Global incidence and mortality of severe fungal disease. Lancet, 400, 1-21. [CrossRef]
• 4. Atanasov, A.G., Zotchev, S.B., Dirsch, V.M., Supuran, C.T. (2021). Natural products in drug discovery: Advances and opportunities. Nature Reviews Drug Discovery, 20(3), 200-216. [CrossRef]
• 5. Zaid, H., Raiyn, J., Nasser, A., Saad, B., Rayan, A. (2010). Physicochemical properties of natural based products versus synthetic chemicals. The Open Nutraceuticals Journal, 3(1), 194-202. [CrossRef]
• 6. Derymedvid, L.V., Horopashna, D.O., Kalko, K.O., Mishchenko, O.Y., Okipniak, I.V., Komissarenko, A.M., Komisarenko, M.A., Sevastianova, T.V. (2021). Anti-inflammatory properties of raspberry shoot extract. Pharmacologyonline, (2), 657-662.
• 7. Komisarenko, M.A., Polischuk, I.M., Upyr, T.V., Saidov, N.B. (2021). Study of amino acid composition and immunomodulatory activity of Rubus idaeus alcoholic extract. Research Journal of Pharmacy and Technology, 14(3), 1329-1332. [CrossRef]
• 8. Krzepiłko, A., Prażak, R., Święciło, A. (2021). Chemical composition, antioxidant and antimicrobial activity of raspberry, blackberry and raspberry-blackberry hybrid leaf buds. Molecules, 26(2), 327. [CrossRef]
• 9. Asnaashari, M., Tajik, R., Khodaparast, M.H.H. (2014). Antioxidant activity of raspberry (Rubus fruticosus) leaves extract and its effect on oxidative stability of sunflower oil. Journal of Food Science and Technology, 52(8), 5180-5187. [CrossRef]
• 10. Veljkovic, B., Djordjevic, N., Dolicanin, Z., Licina, B., Topuzovıc, M., Stankovıc, M., Zlatıc, N., Dajıc-Stevanovıc, Z. (2018). Antioxidant and anticancer properties of leaf and fruit extracts of the wild raspberry (Rubus idaeus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(2), 359-367. [CrossRef]
• 11. Maslov, O.Y., Komisarenko, M.A., Kolisnyk, O.V., Ponomarenko, S.V. (2020). Antioxidant activity of green tea leaves (Camellia sinensis L.) liquid extracts. Pharmacologyonline, (3), 291-298.
• 12. Maslov, O., Kolisnyk, S., Komisarenko, M., Golik, M. (2022). Study of total antioxidant activity of green tea leaves (Camellia sinensis L.). Herba Polonica, 68(1), 1-9. [CrossRef]
• 13. Maslov, O.Y., Komisarenko, M.A., Golik, M.Y., Kolisnyk, S.V., Altukhov, A.A., Baiurka, S.V., Karpushina, S.A., Tkachenko, O., Iuliia, K. (2023). Study of total antioxidant capacity of red raspberry (Rubus idaeous L.) shoots. Vitae, 30(1), 1-9. [CrossRef]
• 14. Maslov, O., Komisarenko, M., Kolisnyk, S., Kostina, T., Golik, M., Moroz, V., Tarasenko, D., Akhmedov, E. (2023). Investigation of the extraction dynamic of the biologically active substances of the raspberry (Rubus idaeus L.) shoots. Current Issues in Pharmacy and Medical Sciences, 36(4), 194-198. [CrossRef]
• 15. Maslov, O.Y., Kolesnik, S.V., Komisarenko, M.A., Altukhov, A.A., Dynnyk, K., Kostina, T. (2021). Development and validation of a titrimetric method for quantitative determination of free organic acids in green tea leaves. Pharmakeftiki, 33(4), 304-311.
• 16. Maslov, O. Y., Kolisnyk, S. V., Kostina, T.A., Shovkova, Z.V., Ahmedov, E.Y., Komisarenko, M.A. (2021). Validation of the alkalimetry method for the quantitative determination of free organic acids in raspberry leaves. Journal of Organic and Pharmaceutical Chemistry, 19(1(73)), 53-58. [CrossRef]
• 17. Maslov, O.Y., Kolisnyk S.V., Komissarenko N.A., Kostina T.A. (2021) Development and validation potentiometric method for determination of antioxidant activity of epigallocatechin-3-O-gallate. Pharmacologyonline, (2), 35-42.
• 18. Maslov, O., Komisarenko, M., Kolisnyk, S., Derymedvid, L. (2024b). Evaluation of anti-Inflammatory, antioxidant activities and molecular docking analysis of Rubus idaeus leaf extract. Jordan Journal of Pharmaceutical Sciences, 17(1), 105-122. [CrossRef]
• 19. Maslov, O., Komisarenko, M., Ponomarenko, S., Horopashna, D., Osolodchenko, T., Kolisnyk, S., Derymedvid, L., Shovkova, Z., Akhmedov, E. (2022). Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract. Current Issues in Pharmacy and Medical Sciences, 35(4), 229-235. [CrossRef]
• 20. Maslov O., Kolisnyk S., Komisarenko M., Komisarenko A., Osolodchenko T., Ponomarenko S. (2022). In vitro antioxidant and antibacterial activities of green tea leaves (Camella sinensis l.) liquid extracts. AMI, (2), 64-67.
• 21. Maslov, O., Komisarenko, M., Horopashna, D., Tkachenko, O., Derymedvid, L., Kolisnyk, S., Kolisnyk, O. (2023). Antioxidant activity of red raspberry shoots (Rubus idaeus L.) liquid extracts. Herba Pol, 69(4), 45-53.
• 22. Maslov, O.Y., Kolisnyk, S.V., Komisarenko, M.A., Altukhov, A.A., Dynnyk, K.V., Stepanenko, V.I. (2021). Study and evaluation antioxidant activity of dietary supplements with green tea extract. Current Issues in Pharmacy and Medicine: Science and Practice, 14(2), 215-219. [CrossRef]
• 23. Ispiryan, A., Atkociuniene, V., Makstutiene, N., Sarkinas, A., Salaseviciene, A., Urbonaviciene, D., Viskelis, J., Pakeltiene, R., Raudone, L. (2024). Correlation between antimicrobial activity values and total phenolic content/antioxidant activity in Rubus idaeus L. Plants, 13(4), 504. [CrossRef]
• 24. Stagos, D., Portesis, N., Spanou, C., Mossialos, D., Aligiannis, N., Chaita, E., Panagoulis, C., Reri, E., Skaltsounis, L., Tsatsakis, A.M., Kouretas, D. (2012). Correlation of total polyphenolic content with antioxidant and antibacterial activity of 24 extracts from Greek domestic Lamiaceae species. Food and Chemical Toxicology, 50(11), 4115-4124. [CrossRef]
• 25. Cirovic, T., Barjaktarevic, A., Cupara, S., Mitic, V., Nikolic, J., Jovanovic, V.S. (2019). Antioxidant and antimicrobial activity of sanguisorba minor L. Extracts. Serbian Journal of Experimental and Clinical Research. [CrossRef]
• 26. Katalinic, V., Mozina, S.S., Generalic, I., Skroza, D., Ljubenkov, I., Klancnik, A. (2012). Phenolic profile, antioxidant capacity, and antimicrobial activity of leaf extracts from six Vitis vinifera L. varieties. International Journal of Food Properties, 16(1), 45-60. [CrossRef]
• 27. Chen, C., Long, L., Zhang, F., Chen, Q., Chen, C., Yu, X., Liu, Q., Bao, J., Long, Z. (2018). Antifungal activity, main active components and mechanism of Curcuma longa extract against Fusarium graminearum. Plos One, 13(3), Article e0194284. [CrossRef]