DESMANTHUS VİRGATUS (L.) YAPRAKLARININ ETANOL EKSTRESİ ÜZERİNE İN SİLİKO VE İN VİTRO ANTİDİYABETİK AKTİVİTE ÇALIŞMALARI

Year 2025, Volume: 49 Issue: 3, 714 - 728, 19.09.2025

Tukiran Tukiran , Andika Pramudya Wardana Lilik Farkhiyah Syahrul Hendrawan , Renita Meilasari

https://doi.org/10.33483/jfpau.1553867

https://izlik.org/JA88AX74WX

Abstract

Amaç: Bu çalışma, in siliko ve in vitro (α-glukozidaz inhibisyonu) yöntemlerle D. virgatus bitkisinin yapraklardan elde edilen etanol ekstresinin antidiyabetik etki potansiyelini belirlemeyi amaçlamaktadır.
Gereç ve Yöntem: LC-MS kullanılarak D. virgatus yapraklarının etanol ekstresindeki bileşikler tanımlanmıştır. Antidiyabetik aktivite in silico ve in vitro olarak belirlenmiştir.
Sonuç ve Tartışma: Kullanılan protein kodu (PDB ID) 3AJ7 ve 3W37'dir, daha sonra akarboz pozitif kontrol olarak kullanılmıştır. LC-MS kullanılarak ekstrakttan 160 bileşik tanımlanmıştır. Tanımlanan bileşiklerin tümü silikoda test edilmiş, 3AJ7 proteini kullanılarak 18 bileşik ve 3W37 ile 10 bileşiğin Lipinski'nin kuralını 3A37 proteini için ≤ -8.2 kcal/mol ve 3W37 için ≤ -8.1 kcal/mol bağlanma afinite değeri ile karşıladığı bulunmuştur, bu da akarboza benzerdir. Ayrıca, ekstre ve akarbozun α-glukozidaz inhibitörü etkisi in vitro olarak test edilmiştir, elde edilen IC50 değeri sırasıyla 144.11 ppm'dir ve 3.78 ppm'dir. Ekstrenin etkisi orta kategoriye sokulabilir.

Keywords

Antidiyabetik , Desmanthus virgatus , etanol ekstresi , in siliko , in vitro

Project Number

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IN SILICO AND IN VITRO ANTIDIABETIC ACTIVITY STUDIES ON THE ETHANOL EXTRACT OF DESMANTHUS VIRGATUS (L.) LEAVES

https://izlik.org/JA88AX74WX

Abstract

Objective: In vitro and in silico studies using α-glucosidase activity inhibition aimed at identifying the potential antidiabetic properties of ethanol extracts of D. virgatus leaves.
Material and Method: Compounds in the ethanol extract of D. virgatus leaves were identified by LC-MS. In vitro and in silico methods were used to determine the antidiabetic activity.
Result and Discussion: The protein code (PDB ID) used is 3AJ7 and 3W37, then acarbose was used as a positive control. By using LC-MS,160 compounds were identified from the extract. All of the identified compounds were tested in silico, using 3AJ7 protein 18 compounds and with 3W37 10 compounds were found to fulfill Lipinski's rule with a binding affinity value ≤ -8.2 kcal/mol for 3A37 protein and ≤ -8.1 kcal/mol for 3W37, which is similar to acarbose. In addition, α-glucosidase inhibition was used to assess the antidiabetic effect of the ethanol extract of D. virgatus leaves in vitro, resulting in IC50 values of 144.11 ppm for the extract and 3.78 ppm for acarbose. The IC50 values of the extract were included in the medium category.

Keywords

Antidiabetic , Desmanthus virgatus , ethanol extract , in silico , in vitro

Ethical Statement

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Supporting Institution

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Project Number

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Thanks

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