CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF PHOLIOTA AURIVELLA (BATSCH) P. KUMM.

Abstract

Objective: This study aims to determine the chemical composition of the mushroom Pholiota aurivella by investigating its antimicrobial, antibiofilm, and antioxidant activities. The potential effects of the ethanol extract of the mushroom on a total of 27 different microorganisms, including strains with multidrug resistance (MDR), were examined. Additionally, the biofilm inhibition capacity and free radical scavenging activities of the mushroom extract were tested. Material and Method: The ethanol extract obtained from the P. aurivella sample was subjected to various tests to evaluate its biological activities. Antimicrobial activity was analyzed using disk diffusion and Minimum Inhibitory Concentration (MIC) tests. Antibiofilm activity was assessed based on its capacity to inhibit biofilm formation, while antioxidant activity was tested using the DPPH method. The chemical composition was determined by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Result and Discussion: Our study reveals that P. aurivella is a promising natural agent, especially in terms of its antimicrobial and antibiofilm activities. The extract exhibited antimicrobial activity against 16 different Gram-positive and Gram-negative microorganisms. Expanding zones of inhibition were observed with increasing extract amounts in strains such as Klebsiella pneumoniae and Enterobacter aerogenes, which show multidrug resistance (MDR). In biofilm inhibition tests, the strongest effects were observed in strains of Listeria innocua and Bacillus subtilis DSMZ 1971. On the other hand, the antioxidant activity tested by the DPPH method was quite low; this can be explained by the absence of phenolic compounds in the chemical composition of the extract. GC-MS analysis identified the major components as linoleic acid (59.20%) and ethyl linoleate (17.13%). These findings indicate that P. aurivella, with its antibiofilm and antimicrobial properties, has potential for pharmaceutical applications and may offer an important natural resource for developing treatment options even against MDR pathogens.

Keywords

• Antibiofilm activity
• antimicrobial activities
• antioxidant activity
• GC-MS
• Pholiota aurivella

Thanks

This manuscript is based on the study previously presented as a conference abstract under the title "In Vitro Antimicrobial Activity Screening of Pholiota aurivella and Determination of the Ethanol Extract Composition by Gas Chromatography/Mass Spectrometry" by Kerem Canlı, Ilgaz Akata, Ali Yetgin, Özcan Şimşek, and Ergin Murat Altuner at The Second Japan – Turkey International Symposium on Pharmaceutical and Biomedical Sciences, held in 2017 in Trabzon.

PHOLIOTA AURIVELLA (BATSCH) P. KUMM.’NİN KİMYASAL BİLEŞİMİ VE BİYOLOJİK AKTİVİTELERİ

Abstract

Amaç: Bu çalışma, Pholiota aurivella mantarının antimikrobiyal, antibiyofilm ve antioksidan aktivitelerini araştırarak kimyasal kompozisyonunu belirlemeyi amaçlamaktadır. Çalışmada, mantarın etanol ekstraktının, çoklu ilaç direncine sahip suşlar da dahil olmak üzere toplam 27 farklı mikroorganizma üzerindeki potansiyel etkisi incelenmiştir. Ayrıca, mantar ekstraktının biyofilm inhibisyon kapasitesi ve serbest radikal süpürme aktiviteleri test edilmiştir. Gereç ve Yöntem: P. aurivella örneğinden etanol ile elde edilen ekstrakt, biyolojik aktivitelerini değerlendirmek amacıyla çeşitli testlere tabi tutulmuştur. Antimikrobiyal etkinlik, disk difüzyon ve Minimum İnhibitör Konsantrasyon (MİK) testleri ile analiz edilmiştir. Antibiyofilm aktivitesi, biyofilm oluşumunu inhibe etme kapasitesiyle değerlendirilirken, antioksidan aktivite DPPH yöntemi ile test edilmiştir. Kimyasal kompozisyon ise Gaz Kromatografisi-Kütle Spektrometrisi (GC-MS) analiziyle belirlenmiştir. Sonuç ve Tartışma: Çalışmamız, P. aurivella'nın özellikle antimikrobiyal ve antibiyofilm aktiviteleri açısından umut verici bir doğal ajan olduğunu ortaya koymaktadır. Ekstrakt, 16 farklı Gram-pozitif ve Gram-negatif mikroorganizmaya karşı antimikrobiyal etkinlik gösterirken, çoklu ilaç direnci (MDR) gösteren Klebsiella pneumoniae ve Enterobacter aerogenes gibi suşlarda da artan ekstrakt miktarı ile genişleyen inhibisyon alanları gözlemlenmiştir. Biyofilm inhibisyonu testlerinde de en güçlü etkiler, Listeria innocua ve Bacillus subtilis DSMZ 1971 suşlarında gözlemlenmiştir. Öte yandan, DPPH yöntemiyle test edilen antioksidan aktivite oldukça düşüktür; bu durum, ekstraktın kimyasal bileşiminde fenolik bileşiklerin bulunmamasıyla açıklanabilir. GC-MS analizinde, majör bileşenlerin linoleik asit (%59.20) ve etil linoleat (%17.13) olduğu tespit edilmiştir. Bu bulgular, P. aurivella'nın antibiyofilm ve antimikrobiyal özellikleriyle farmasötik uygulamalar açısından potansiyele sahip olduğunu ve MDR patojenlere karşı dahi tedavi seçenekleri geliştirilmesinde önemli bir doğal kaynak sunabileceğini göstermektedir.

Keywords

• Antibyiofilm aktivite
• antimikrobiyal aktivite
• antioksidan aktivite
• GC-MS
• Pholiota aurivella

Thanks

Bu makale, Kerem Canlı, Ilgaz Akata, Ali Yetgin, Özcan Şimşek ve Ergin Murat Altuner tarafından "In Vitro Antimicrobial Activity Screening of Pholiota aurivella and Determination of the Ethanol Extract Composition by Gas Chromatography/Mass Spectrometry" başlığıyla 2017 yılında Trabzon'da düzenlenen The Second Japan – Turkey International Symposium on Pharmaceutical and Biomedical Sciences adlı sempozyumda bildiri olarak sunulan çalışmaya dayanmaktadır.

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