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KÜMES HAYVANLARI YUMURTALARINDAKİ PİPERİDİN-1-YUM {[5-(2-FURİL)-4- FENİL-4H-1,2,4-TRİAZOL-3-İL]TİYO}ASETAT ARTIKLARININ BELİRLENMESİ İÇİN BİR LC-ESI-MS YÖNTEMİNİN GELİŞTİRİLMESİ VE VALİDASYONU

Year 2016, , 29 - 40, 01.12.2016

Borys Varynskyı

https://doi.org/10.1501/Eczfak_0000000586

Abstract

ESI-MS yöntemi tasarlanmıştır. Numuneler ultrasonik banyoda dimetil sülfoksit ile ekstre edilmiş, 15.000 g'da
santrifüjlenmiş, ultrafiltre ile süzülmüş ve HPLC sistemine enjekte edilmiştir. Suda %0,1 (h/h) formik asit (A)
ve asetonitrilde %0,1 (h/h) formik asit (B) içeren mobil faz ile izokratik elüsyon yapılmıştır. Mobil fazın
bileşimi, 0,4 mL. dak-1 akış hızında, A/B (%70: %30, h/h)dır. Toplam çalışma süresi 5 dakikadır. Kütle
spektrometrik koşullar, SIM modu (m/z 302,1), pozitif polaritedir. Doğruluk %0,2887-7,204 dır. Kesinlik,
%1,564-5,636 dır. Yöntem, kümes hayvanları yumurtalarındaki Trifuzol kalıntısı kantitatif tayininde
kullanılabilir.

Keywords

kümes hayvanları yumurtası kütle spektrometrisi triazoller trifuzol yüksek basınçlı sıvı kromatografisi

DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS

Year 2016, , 29 - 40, 01.12.2016

Borys Varynskyı

https://doi.org/10.1501/Eczfak_0000000586

Abstract

A simple, rapid and reproducible isocratic reversed-phase LC-ESI-MS method has been elaborated for
determination of the piperidin-1-ium {[5-(2-furyl)-4-phenyl-4H-1,2,4-triazol-3-yl] thio}acetate, active
pharmaceutical ingredient (API) of the antiviral veterinary drug “Tryfuzol” in the poultry eggs. The samples
were extracted by dimethyl sulfoxide at ultrasonic bath, centrifuged at 15000 g, filtrated through ultrafilter and
injected into HPLC system. A mobile phase was used 0.1% (v/v) formic acid in water (A) and acetonitrile
containing 0.1% (v/v) formic acid (B) at isocratic elution. The composition of the mobile phase was A/B
(70%:30%, v/v) at flow rate of 0.4 ml min-1. Total running time was 5 minutes. Mass spectrometric conditions
were SIM mode (m/z 302.1), positive polarity. The mean concentration were 0.2887-7.204% of the nominal
values for the QC samples. The within-run CV values were 1.564-5.636% for the QC samples. The method is
applicable for determination of the residual quantities of API of the “Tryfuzol” in the poultry eggs.

Keywords

ESI mass spectrometry high pressure liquid chromatography poultry eggs triazoles tryfuzol

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Details

Other ID JA74DS36KH
Journal Section Research Article
Authors

Borys Varynskyı This is me

Publication Date December 1, 2016
Submission Date December 1, 2016
Published in Issue Year 2016

Cite

APA Varynskyı, B. (2016). DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS. Journal of Faculty of Pharmacy of Ankara University, 40(3), 29-40. https://doi.org/10.1501/Eczfak_0000000586
AMA Varynskyı B. DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS. Ankara Ecz. Fak. Derg. December 2016;40(3):29-40. doi:10.1501/Eczfak_0000000586
Chicago Varynskyı, Borys. “DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS”. Journal of Faculty of Pharmacy of Ankara University 40, no. 3 (December 2016): 29-40. https://doi.org/10.1501/Eczfak_0000000586.
EndNote Varynskyı B (December 1, 2016) DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS. Journal of Faculty of Pharmacy of Ankara University 40 3 29–40.
IEEE B. Varynskyı, “DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS”, Ankara Ecz. Fak. Derg., vol. 40, no. 3, pp. 29–40, 2016, doi: 10.1501/Eczfak_0000000586.
ISNAD Varynskyı, Borys. “DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS”. Journal of Faculty of Pharmacy of Ankara University 40/3 (December 2016), 29-40. https://doi.org/10.1501/Eczfak_0000000586.
JAMA Varynskyı B. DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS. Ankara Ecz. Fak. Derg. 2016;40:29–40.
MLA Varynskyı, Borys. “DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS”. Journal of Faculty of Pharmacy of Ankara University, vol. 40, no. 3, 2016, pp. 29-40, doi:10.1501/Eczfak_0000000586.
Vancouver Varynskyı B. DEVELOPMENT AND VALIDATION OF A LC-ESI-MS METHOD FOR DETECTION OF PIPERIDIN-1-IUM {[5-(2-FURYL)-4-PHENYL-4H- 1,2,4-TRIAZOL-3-YL] THIO}ACETATE RESIDUES IN POULTRY EGGS. Ankara Ecz. Fak. Derg. 2016;40(3):29-40.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.