GLİSİN İLE TİYOTRİAZOLİN SABİT KOMBİNASYONUNUN DENEYSEL SEREBRAL İSKEMİ ŞARTLARINDA BEYİN ENERJİ METABOLİZMASI GÖSTERGELERİNE ETKİSİ

Abstract

Amaç: Serebral iskemide primer nörokoruma uyarıcı ve inhibitor nörotransmiter sistemleri arasındaki bozulan dengenin doğal inhibitor yolakların aktivasyonu ile düzeltilmesi yaklaşımına dayanır. Antioksidan bir madde olan tiyotriazolinin nöro-metabolik serebral koruyucuların terapötik etkilerini arttırdığına ilişkin kanıtlar bulunmaktadır. Bu nedenle glisin ve tiyotriazolini birlikte içeren yeni bir ilaç kombinasyonu oluşturmak ilginç olabilir. Bu çalışmada glisinin, ve tiyotriazolin ile kombine kullanımının akut serebrovasküler bozukluk (ASVB) oluşturulmuş rat modelinde hidrokarbon-enerji prosesleri ve oksidatif metabolizma parametreleri üzerindeki etkilerinin araştırılması amaçlanmıştır.

Gereç ve Yöntem: ASVB, klasik yöntem olan ana karotis arterlerin simültane ligasyonu ile gerçekleştirilmiş ve deneylerde 50 erkek Wistar rat kullanılmıştır. Uygulamalara gruplardaki ratlar anesteziden çıktıktan hemen sonra intraperitonal olarak başlanmış ve 4 gün boyunca devam edilmiştir. Beyin kortekslerinde adenil nükleotidler, piruvat, laktat, malat ve isositrat düzeyleri, suksinat dehidrogenaz, sitokrom C-oksidaz, glutamat dekarboksilaz ve GABA-transferaz aktiviteleri biyokimyasal yöntemlerle saptanmıştır.

Sonuç ve Tartışma: Glisin ve tiyotriazolinin birlikte kullanımının, beyin enerji metabolizması göstergeleri üzerine referans ilaçlar olan pirasetam ve glisinden daha etkili olması, bu kombinasyon üzerinde araştırmaların devam etmesi gerekliliğini göstermektedir.

Keywords

• amino asit transmitterler,glisin,inme,nörokoruyucu etkinlik,tiyotriazolin

INFLUENCE OF THE FIXED COMBINATION OF GLYCINE WITH THIOTRIAZOLINE ON ENERGY METABOLISM PARAMETERS IN BRAIN IN CONDITIONS OF EXPERIMENTAL CEREBRAL ISCHEMIA

Abstract

Objective: The perspective direction of primary neuroprotection in cerebral ischemia is the correction of the imbalance of excitatory and inhibitory neurotransmitter systems by activating natural inhibitory processes. There is evidence of the ability of anti-oxidant thiotriazoline to potentiate the therapeutic effect of neuro-metabolic cerebroprotectors. Therefore, it is interesting to create a new combined drug based on glycine and thiotriazoline. The purpose of this study is to investigate the effect of glycine, as well as its combination with thiotriazoline, on the parameters of hydrocarbon-energy processes and oxidative metabolism under the conditions of simulation of acute cerebrovascular disorder (ACVD).

Material and Method: To create ACVD, a classic model consisting of simultaneous ligation of common carotid arteries was used in 50 Wistar male rats. All drugs were administered intraperitonally for four days starting with anesthesia recovery of rat groups. The content of adenyl nucleotides, pyruvate, lactate, malate, isocitrate and activities of succinate dehydrogenase, cytochrome C-oxidase, glutamate decarboxylase, GABAtransferase were determined in the homogenates of brain cortex by biochemical methods.

Result and Discussion: Our results showed that the combination of glycine with thiotriazoline is better than such reference drugs like pyracetam and glycine, according to degree of effect on the indicators of energy metabolism of the brain, indicating the relevance of further study of the proposed combination. 

Keywords

• glycine,neuroprotective action,stroke,thiotriazoline,transmitter,amino acids

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