BAZI NAFTOKİNON TÜREVLERİ İLE İLAÇ MADDELERİ ARASINDAKİ ETKİLEŞİM ÜRÜNLERİNİN YAPISININ İNCELENMESİ

Abstract

Amaç: Naftokinon esaslı analitik reaktiflerin asetilsistein, β-alanin ve taurin (2-aminoetanosülfonik asit) gibi farmasötik açıdan aktif maddelerle etkilişimin, oluşan ürünlerin ayırımı yapı oluşumu dahil olmak üzere detaylı incelemesidir.

Gereç ve Yöntem: Farmasötik maddeler ile naftokinon türevleri arasındaki reaksiyonlarda reaktanların stokiyometrik katsayılarını belirlemek için UV - Vis spektrofotometri yöntemi uygulandı. Reaksiyon ürünlerinin yapısını kanıtlamak için 1Н NMR-spektroskopi, IR spektroskopi, kütle spektrometrisi kullanıldı.

Sonuç ve Tartışma: Kinon türevleri ile araştırılan tıbbi maddelerin reaksiyonlarında reaktanların stokiyometrik ilişkileri, doyurma ve sürekli değişim yöntemleri ile belirlenmiştir. İlişki her durumda 1: 1'dir. Bileşen reaksiyonu ve reaksiyon yolu için optimal koşulların belirlenen ilişkilerine göre, asetilsisteinin 2,3-dikloro-1,4-naftokinon ve taurin ile, β-alanin ile sodyum 1,2-naftokinon-4-sülfonat ile etkileşim ürünleri izole edilmiş ve tanımlanmıştır. Bileşiklerin yapısını oluşturmak için 1Н NMR-spektroskopi, IR spektroskopisi, kütle spektrometrisi kullanılmıştır.

Keywords

• Asetilsistein
• β-alanin
• taurin
• naftokinon türevleri
• reaksiyon ürünleri

STUDY OF THE STRUCTURE OF PRODUCTS OF INTERACTION BETWEEN SOME NAPHTHOQUINONE DERIVATIVES AND PHARMACEUTICAL SUBSTANCES

Abstract

Objective: The purpose of the present work is to detail а study of the interaction between naphthoquinone-based analytical reagents and such pharmacologically active ingredients as acetylcysteine, β-alanine and taurine (2-aminoethanesulfonic acid) including the isolation and structure elucidation of the formed products.

Material and Method: UV-visible spectrophotometry was applied to determine the stoichiometric coefficients of reactants in reactions between the pharmaceutical substances and naphthoquinone derivatives. 1Н NMR-spectroscopy, IR spectroscopy, сhromatomass-spectrometry were used to prove the structure of the reaction products.

Result and Discussion: The stoichiometric relationships of the reactants in the investigated medicinal substances reactions with quinone derivatives were determined by the methods of saturation and continuous changes. They are 1:1 in each case. According to the determined relationships of components reaction and optimal conditions for reactions path, the products of interaction of acetylcysteine with 2,3-dichloro-1,4-naphthoquinone and taurine, β-alanine with sodium 1,2-naphthoquinone-4-sulfonate were isolated and identified. In order to establish the structure of the compounds, the 1Н NMR-spectroscopy, IR spectroscopy, сhromatomass-spectrometry were used.

Keywords

• Acetylcysteine
• β-alanine
• taurine
• naphthoquinone derivatives
• reaction products

References

1. 1. Abou-Ouf, A., Taha, A. M., Saidhom, M. (1973). Spectrophotometric analysis of primary aliphatic amines with dichlone. Journal of Pharmaceutical Sciences, 62(10), 1700-1702. doi:10.1002/jps.2600621028
2. 2. C. J. Shishoo, B. N. Suhagia, I. S. Rathod, S. S. Thakore. (1996). Spectrophotometric estimation of piperazine in dosage forms using Dichlone and Acetaldehyde as reagents. Indian Journal of Pharmaceutical Sciences, 58(6), 219-221.
3. 3. El-Yazbi, F. A., Abdine, H. H., Shaalan, R. A. (1999). Spectrophotometric and spectrofluorometric methods for the assay of lisinopril in single and multicomponent pharmaceutical dosage forms. Journal of Pharmaceutical and Biomedical Analysis, 19(6), 819-827. doi:10.1016/s0731-7085(98)00110-1
4. 4. Li, Q., Yang, Z. (2007). Spectrophotometric determination of aminomethylbenzoic acid using sodium 1,2-naphthoquinone-4-sulfonate as the chemical derivative chromogenic reagent. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 66(3), 656-661. doi:10.1016/j.saa.2006.04.007
5. 5. Xu, L., Wang, H., Xiao, Y. (2004). Spectrophotometric determination of ampicillin sodium in pharmaceutical products using sodium 1,2-naphthoquinone-4-sulfonic as the chromogentic reagent. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 60(13), 3007-3012. doi:10.1016/j.saa.2004.02.018
6. 6. Saurina, J., Hernández-Cassuo, S. (1993). Continuous-flow spectrophotometric determination of amino acids with 1,2-naphthoquinone-4-sulphonate reagent. Analytica Chimica Acta, 283(1), 414-420. doi:10.1016/0003-2670(93)85252-f
7. 7. Miedviedieva, K. P., Donchenko, А О, Vasiuk, S. O. (2019). Application of quinone derivatives for spectrophotometric determination of drugs. Current Issues in Pharmacy and Medicine: Science and Practice, 0(3). doi:10.14739/2409-2932.2019.3.184173
8. 8. Portnaya, E. P., Vasyuk, S. A. (2014). Spectrophotometric determination of taurine in dosage forms using sodium 1,2-naphthoquinone-4-sulfonate. Pharmaceutical Chemistry Journal, 48(8), 553-557. doi:10.1007/s11094-014-1150-3