SPECTROPHOTOMETRIC DETERMINATION OF ACTIVE COMPOUNDS IN AN ANTIHYPERTENSIVE FORMULATION BY HAAR-CWT METHOD

Abstract

Objective: In present work, we developed a new wavelet method (HAAR-CWT) to determine the amounts of irbesartan (IR) and hydrochlorotizide (HC) active compounds simultaneously in an antihypertensive formulation, and after validating this method, we successfully applied it to the assay of tablets containing the above-mentioned compounds.

Material and Method: The absorption spectra of mixtures and tablet solutions in the spectral range 200-305 nm were measured by a Shimadzu UV-1601 dual-beam UV-VIS spectrophotometer.

Result and Discussion: Due to the strong overlapping spectra of the mentioned components, the HAAR-CWT method has been proposed and successfully applied to the ratio spectra of IR-HC mixtures and tablets.

Keywords

• Spectrophotometry
• Quantitative determination
• Wavelet transform
• Irbesartan
• Hydrochlorothiazide

BİR ANTİHİPERTANSİF FORMÜLASYONDAKİ AKTİF BİLEŞİKLERİN HAAR-CWT YÖNTEMİYLE SPEKTROFOTOMETRİK TAYİNİ

Abstract

Amaç: Bu çalışmada, bir antihipertansif formülasyondaki irbesartan (IR) ve hidroklorotizid (HC) aktif bileşiklerinin miktarlarını aynı anda belirlemek için yeni bir dalgacık yöntemi (HAAR-CWT) geliştirdik ve bu yöntemi doğruladıktan sonra, yukarıda bahsedilen bileşikleri içeren tabletlerin analizine başarıyla uyguladık.

Gereç ve Yöntem: 200-305 nm spektral aralığındaki karışımların ve tablet çözeltilerinin absorpsiyon spektrumları, bir Shimadzu UV-1601 çift ışınlı UV-VIS spektrofotometresi ile ölçülmüştür.

Sonuç ve Tartışma: Bahsedilen bileşenlerin güçlü şekilde örtüşen spektrumları nedeniyle, HAAR-CWT yöntemi önerilmiş ve IR-HC karışımlarının ve tabletlerinin oran spektrumlarına başarıyla uygulanmıştır.      

Keywords

• Spektrofotometri
• kantitatif tayin
• dalgacık dönüşümü
• irbesartan
• hidroklorotiyazid

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