TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI

Hande Yüce; Neşe Başak Türkmen; Dilan Aşkın Özek; Songül Ünüvar

doi:10.33483/jfpau.995491

Research Article

TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI

Year 2022, Volume: 46 Issue: 1, 48 - 61, 29.01.2022

Hande Yüce , Neşe Başak Türkmen , Dilan Aşkın Özek , Songül Ünüvar

https://doi.org/10.33483/jfpau.995491

https://izlik.org/JA23EY33TS

Abstract

Amaç: Bu çalışmanın amacı, taurin, kapsaisin, melatonin ve beta karoten gibi antioksidan maddelerin insan meme kanseri ve sağlıklı fibroblast hücreleri üzerindeki antiproliferatif, antimigrasyon ve antioksidan aktivitelerini değerlendirmektir.
Gereç ve Yöntem: Bu çalışmada, taurin, kapsaisin, melatonin ve beta karoten L929 sağlıklı fibroblast hücrelerine ve MCF-7 meme kanseri hücrelerine uygulandı. Bileşiklerin antiproliferatif etkisi, MTS [3-(4,5-dimetiltiazol-2-il)-5-(3-karboksimetoksifenil)-2-(4sülfofenil)-2H-tetrazolyum] testi ile 24. ve 48. saatte belirlendi. Hücreler, Dulbecco'nun Modifiye Eagles Ortamı (DMEM) içinde kültürlendi ve ardışık iki gün boyunca farklı konsantrasyonlarla işleme tabi tutuldu. Bileşiklerin hücre göçü üzerindeki etkisi, yara iyileşme deneyi kullanılarak 24. saatte değerlendirildi. Yara iyileşme testi, hücrelerin in vitro metastatik kabiliyetini ölçmek için kullanıldı. Total antioksidan seviyeleri (TAS) ve total oksidan seviyeleri (TOS) ticari kitler kullanılarak belirlendi.
Sonuç ve Tartışma: Tüm bileşikler sağlıklı hücrelere kıyasla, malign hücrelerde hücre canlılığını konsantrasyona ve zamana bağlı bir şekilde azaltmıştır. Yara kapanma alanının sonuçları, bileşiklerle tedavinin, yara kapanmasını önemli ölçüde hızlandıran hücresel göçü iyileştirdiğini göstermiştir. Sonuçlar, tüm bileşiklerin, 48 saatte MCF-7 hücre hatlarının göç kabiliyetini belirgin şekilde inhibe ettiğini gösterdi. Tüm bileşikler antioksidan etki göstermekle birlikte, MCF-7 hücreleri üzerine hemen hemen tüm dozlarda antioksidan etki gösteren bileşiğin beta karoten olduğu gözlenmiştir.

Keywords

Beta karoten , kapsaisin , melatonin , meme kanseri , taurin

References

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3. Wang, H., Khor, T.O., Shu, L., Su, Z.Y., Fuentes, F., Lee, J.H., Kong, A.N. (2012). Plants vs. cancer: a review on natural phytochemicals in preventing and treating cancers and their druggability. Anti-cancer Agents in Medicinal Chemistry, 12(10), 1281–1305. [CrossRef]
4. Chou, C.C., Wu, Y.C., Wang, Y.F., Chou, M.J., Kuo, S.J., Chen, D.R. (2009). Capsaicin-induced apoptosis in human breast cancer MCF-7 cells through caspase-independent pathway. Oncology Reports, 21(3), 665–671. [CrossRef]
5. Eghdami, A., Salehi, M.A., Babakhani, M. (2014). Determination of physicochemical properties of capsaicin and cytotoxic effect of capsicum extract in breast cancer (MCF7) cell line. International Journal of Biosciences, 4(8), 262-268. [CrossRef]
6. Sowmya, S.G., Yogendra, P.K., Arpitha, H.S., Deepika, U.R., Nawneet, K.K., Mondal, P., Ganesan, P. (2017). β-carotene at physiologically attainable concentration induces apoptosis and down-regulates cell survival and antioxidant markers in human breast cancer (MCF-7) cells. Molecular and Cellular Biochemistry, 436(1-2), 1-12. [CrossRef]
7. Yeh, S.L., Wang, H.M., Chen, P.Y., Wu, T.C. (2009). Interactions of b-carotene and flavonoids on the secretion of pro-inflammatory mediators in an in vitro system. Chemico-Biological Interactions, 179(2-3), 386-393. [CrossRef]
8. Gloria, N.F., Soares, N., Brand, C., Oliveira, F.L., Borojevic, R., Teodoro, A.J. (2014). Lycopene and beta-carotene induce cell-cycle arrest and apoptosis in human breast cancer cell lines. Anticancer Research, 34(3), 1377-1386. [CrossRef]
9. Zhang, X., Lu, H., Wang, Y., Liu, C., Zhu, W., Zheng, S., Wan, F. (2015). Taurine induces the apoptosis of breast cancer cells by regulating apoptosis-related proteins of mitochondria. International Journal of Molecular Medicine, 35(1), 218–226. [CrossRef]
10. Tu, S., Zhang, X.L., Wan, H.F., Xia, Y.Q., Liu, Z.Q., Yang, X.H., Wan, F.S. (2018). Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells. Oncology Letters, 15(4), 5473–5480. [CrossRef]
11. Gatti, G., Lucini, V., Dugnani, S., Calastretti, A., Spadoni, G., Bedini, A., Rivara, S., Mor, M., Canti, G., Scaglione, F., Bevilacqua, A. (2017). Antiproliferative and pro-apoptotic activity of melatonin analogues on melanoma and breast cancer cells. Oncotarget, 8(40), 68338–68353. [CrossRef]
12. Barltrop, J.A., Owen, T.C., Cory, A.H., Cory, J.G. (1991). 5-(3- carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3- (4-sulfophenyl) tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5- diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans as cell-viability indicators. Bioorganic&Medicinal Chemistry Letters, 1(11), 611-614. [CrossRef]
13. Liang, C.C., Park, A., Guan, J.L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329–333. [CrossRef]
14. Ammerman, N.C., Beier-Sexton, M., Azad, A.F. (2008). Growth and maintenance of Vero cell lines. Current protocols in microbiology, Appendix 4, Appendix–4E. [CrossRef]
15. Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103–1111. [CrossRef]
16. Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277–285. [CrossRef]
17. El Agouza, I.M., Eissa, S.S., El Houseini, M.M., El-Nashar, D.E., Abd El Hameed, O.M. (2011). Taurine: A novel tumor marker for enhanced detection of breast cancer among female patients. Angiogenesis, 14(3), 321–330. [CrossRef]
18. Zhang, X., Lu, H., Wang, Y., Liu, C., Zhu, W., Zheng, S., Wan, F. (2015). Taurine induces apoptosis of breast cancer cells by regulating apoptosis-related proteins of mitochondria. International Journal of Molecular Medicine, 35(1), 218–226. [CrossRef]
19. Okamoto, K., Sugie, S., Ohnishi, M., Makita, H., Kawamori, T., Watanabe, T., Tanaka, T., Mori, H. (1996). Chemopreventive effects of taurine on diethylnitrosamine and phenobarbital-induced hepatocarcinogenesis in male F344 rats. Japanese Journal of Cancer Research, 87(1), 30–36. [CrossRef]
20. Wang, H.R. (2008). PhD Thesis. Experimental study of the effect of taurine on sarcoma 180(S180) in mices. Qingdao University, Qingdao, China.
21. Neary, P.M., Hallihan, P., Wang, J.H., Pfirrmann, R.W., Bouchier-Hayes, D.J., Redmond, H.P. (2010). The evolving role of taurolidine in cancer therapy. Annals of Surgical Oncology, 17(4), 1135–1143. [CrossRef]
22. Popescu, G.D.A., Scheau, C., Badarau, I.A., Dumitrache, M.D., Caruntu, A., Scheau, A.E., Costache, D.O., Costache, R.S., Constantin, C, Neagu, M., Caruntu, C. (2020). The effects of capsaicin on gastrointestinal cancers. Molecules, 26(1), 94. [CrossRef]
23. Zhang, R., Humphreys, I., Sahu, R.P., Shi, Y., Srivastava, S.K. (2008). In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis, 13(12), 1465-1478. [CrossRef]
24. Popescu, G., Scheau, C., Badarau, I. A., Dumitrache, M. D., Caruntu, A., Scheau, A. E., Costache, D. O., Costache, R. S., Constantin, C., Neagu, M., Caruntu, C. (2020). The Effects of Capsaicin on Gastrointestinal Cancers. Molecules (Basel, Switzerland), 26(1), 94. [CrossRef]
25. Cory, H., Passarelli, S., Szeto, J., Tamez, M., Mattei, J. (2018). The role of polyphenols in human health and food systems: A Mini-Review. Frontiers in Nutrition, 5, 87. [CrossRef]
26. Surh, Y.J., Lee, S.S. (1995). Capsaicin, a double-edged sword: toxicity, metabolism, and chemopreventive potential. Life Sciences, 56(22), 1845–1855. [CrossRef]
27. Fattori, V., Hohmann, M.S., Rossaneis, A.C., Pinho-Ribeiro, F.A., Verri, W.A. (2016). Capsaicin: Current understanding of its mechanisms and therapy of pain and other pre-clinical and clinical uses. Molecules (Basel, Switzerland), 21(7), 844. [CrossRef]
28. Bouayed, J., Bohn, T. (2010). Exogenous antioxidants--Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxidative Medicine and Cellular Longevity, 3(4), 228–237. [CrossRef]
29. Cooper, D.A., Eldridge, A.L., Peters, J.C. (1999). Dietary carotenoids and certain cancers, heart disease, and age-related macular degeneration: a review of recent research. Nutrition Reviews, 57(7), 201–214. [CrossRef]
30. Hirsch, K., Atzmon, A., Danilenko, M., Levy, J., Sharoni, Y. (2007). Lycopene and other carotenoids inhibit estrogenic activity of 17beta-estradiol and genistein in cancer cells. Breast Cancer Research and Treatment, 104(2), 221–230. [CrossRef]
31. Larsson, S.C., Bergkvist, L., Wolk, A. (2010). Dietary carotenoids and risk of hormone receptor-defined breast cancer in a prospective cohort of Swedish women. European Journal of Cancer, 46(6), 1079–1085. [CrossRef]
32. Zhang, X., Spiegelman, D., Baglietto, L., Bernstein, L., Boggs, D.A., van den Brandt, P.A., Buring, J.E., Gapstur, S.M., Giles, G.G., Giovannucci, E., Goodman, G., ... Smith-Warner, S.A. (2012). Carotenoid intakes and risk of breast cancer defined by estrogen receptor and progesterone receptor status: a pooled analysis of 18 prospective cohort studies. The American Journal of Clinical Nutrition, 95(3), 713–725. [CrossRef]
33. Tordjman, S., Chokron, S., Delorme, R., Charrier, A., Bellissant, E., Jaafari, N., Fougerou, C. (2017). Melatonin: Pharmacology, Functions and Therapeutic Benefits. Current Neuropharmacology, 15(3), 434-443. [CrossRef]
34. Menéndez-Menéndez, J., Martínez-Campa, C. (2018). Melatonin: An anti-tumor agent in hormone-dependent cancers. International Journal of Endocrinology, 3271948. [CrossRef]
35. Nowfar, S., Teplitzky, S.R., Melancon, K., Kiefer, T.L., Cheng, Q., Dwived, P.D., Bischoff, E.D., Moro, K., Anderson, M.B., Dai, J., Lai, L., Yuan, L., Hill, S.M. (2002). Tumor prevention by 9-cis-retinoic acid in the N-nitroso-N-methylurea model of mammary carcinogenesis is potentiated by the pineal hormone melatonin. Breast Cancer Research and Treatment, 72(1), 33-43. [CrossRef]
36. Najafi, M., Salehi, E., Farhood, B., Nashtaei, M.S., Hashemi Goradel, N., Khanlarkhani, N., Namjoo, Z., Mortezaee, K. (2019). Adjuvant chemotherapy with melatonin for targeting human cancers: A review. Journal of Cellular Physiology, 234(3), 2356–2372. [CrossRef]

THE INVESTIGATION OF THE ANTIPROLIFERATIVE, ANTIMIGRATION AND ANTIOXIDANT EFFECTS OF TAURINE, CAPSAICIN, MELATONIN AND BETA CAROTEN ON L929 HEALTHY CELLS AND MCF-7 BREAST CANCER CELLS

Year 2022, Volume: 46 Issue: 1, 48 - 61, 29.01.2022

Hande Yüce , Neşe Başak Türkmen , Dilan Aşkın Özek , Songül Ünüvar

https://doi.org/10.33483/jfpau.995491

https://izlik.org/JA23EY33TS

Abstract

Objective: The aim of this study was to evaluate the antiproliferative, antimigration and antioxidant activities of antioxidant substances such as taurine, capsaicin, melatonin and beta carotene on human breast cancer cells.
Material and Method: In this study, taurine, capsaicin, melatonin and beta carotene L929 were applied to healthy fibroblast cells and MCF-7 breast cancer cells. The antiproliferative effect of the compounds was determined by the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4sulphophenyl)-2H-tetrazolium] test at 24 and 48 hours. Cells were cultured in Dulbecco's Modified Eagles Medium (DMEM) and treated with different concentrations for two consecutive days. The effect of compounds on cell migration was evaluated at 24 hours using the wound healing assay. The wound healing test was used to measure the in vitro metastatic ability of cells. Total antioxidant levels (TAS) and total oxidant levels (TOS) were determined using commercial kits.
Result and Discussion: All compounds decreased cell viability in malignant cells in a concentration- and time-dependent manner compared to healthy cells. The results of the wound closure area showed that treatment with the compounds improved cellular migration, which significantly accelerated wound closure. The results showed that all compounds markedly inhibited the migratory ability of MCF-7 cell lines at 48 hours. Although all compounds showed antioxidant effects, beta carotene was observed to have antioxidant effects on MCF-7 cells at almost all doses.

Keywords

Beta carotene , breast cancer , capsaicin , melatonin , taurine

References

1. Ginsburg, O., Bray, F., Coleman, M. P., Vanderpuye, V., Eniu, A., Kotha, S. R., Sarker, M., Huong, T. T., Allemani, C., Dvaladze, A., Gralow, J., ... Conteh, L. (2017). The global burden of women's cancers: a grand challenge in global health. Lancet (London, England), 389(10071), 847–860. [CrossRef]
2. Schirrmacher, V. (2019). From chemotherapy to biological therapy: A review of novel concepts to reduce the side effects of systemic cancer treatment (Review). International Journal of Oncology, 54, 407-419. [CrossRef]
3. Wang, H., Khor, T.O., Shu, L., Su, Z.Y., Fuentes, F., Lee, J.H., Kong, A.N. (2012). Plants vs. cancer: a review on natural phytochemicals in preventing and treating cancers and their druggability. Anti-cancer Agents in Medicinal Chemistry, 12(10), 1281–1305. [CrossRef]
4. Chou, C.C., Wu, Y.C., Wang, Y.F., Chou, M.J., Kuo, S.J., Chen, D.R. (2009). Capsaicin-induced apoptosis in human breast cancer MCF-7 cells through caspase-independent pathway. Oncology Reports, 21(3), 665–671. [CrossRef]
5. Eghdami, A., Salehi, M.A., Babakhani, M. (2014). Determination of physicochemical properties of capsaicin and cytotoxic effect of capsicum extract in breast cancer (MCF7) cell line. International Journal of Biosciences, 4(8), 262-268. [CrossRef]
6. Sowmya, S.G., Yogendra, P.K., Arpitha, H.S., Deepika, U.R., Nawneet, K.K., Mondal, P., Ganesan, P. (2017). β-carotene at physiologically attainable concentration induces apoptosis and down-regulates cell survival and antioxidant markers in human breast cancer (MCF-7) cells. Molecular and Cellular Biochemistry, 436(1-2), 1-12. [CrossRef]
7. Yeh, S.L., Wang, H.M., Chen, P.Y., Wu, T.C. (2009). Interactions of b-carotene and flavonoids on the secretion of pro-inflammatory mediators in an in vitro system. Chemico-Biological Interactions, 179(2-3), 386-393. [CrossRef]
8. Gloria, N.F., Soares, N., Brand, C., Oliveira, F.L., Borojevic, R., Teodoro, A.J. (2014). Lycopene and beta-carotene induce cell-cycle arrest and apoptosis in human breast cancer cell lines. Anticancer Research, 34(3), 1377-1386. [CrossRef]
9. Zhang, X., Lu, H., Wang, Y., Liu, C., Zhu, W., Zheng, S., Wan, F. (2015). Taurine induces the apoptosis of breast cancer cells by regulating apoptosis-related proteins of mitochondria. International Journal of Molecular Medicine, 35(1), 218–226. [CrossRef]
10. Tu, S., Zhang, X.L., Wan, H.F., Xia, Y.Q., Liu, Z.Q., Yang, X.H., Wan, F.S. (2018). Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells. Oncology Letters, 15(4), 5473–5480. [CrossRef]
11. Gatti, G., Lucini, V., Dugnani, S., Calastretti, A., Spadoni, G., Bedini, A., Rivara, S., Mor, M., Canti, G., Scaglione, F., Bevilacqua, A. (2017). Antiproliferative and pro-apoptotic activity of melatonin analogues on melanoma and breast cancer cells. Oncotarget, 8(40), 68338–68353. [CrossRef]
12. Barltrop, J.A., Owen, T.C., Cory, A.H., Cory, J.G. (1991). 5-(3- carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3- (4-sulfophenyl) tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5- diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans as cell-viability indicators. Bioorganic&Medicinal Chemistry Letters, 1(11), 611-614. [CrossRef]
13. Liang, C.C., Park, A., Guan, J.L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329–333. [CrossRef]
14. Ammerman, N.C., Beier-Sexton, M., Azad, A.F. (2008). Growth and maintenance of Vero cell lines. Current protocols in microbiology, Appendix 4, Appendix–4E. [CrossRef]
15. Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38(12), 1103–1111. [CrossRef]
16. Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277–285. [CrossRef]
17. El Agouza, I.M., Eissa, S.S., El Houseini, M.M., El-Nashar, D.E., Abd El Hameed, O.M. (2011). Taurine: A novel tumor marker for enhanced detection of breast cancer among female patients. Angiogenesis, 14(3), 321–330. [CrossRef]
18. Zhang, X., Lu, H., Wang, Y., Liu, C., Zhu, W., Zheng, S., Wan, F. (2015). Taurine induces apoptosis of breast cancer cells by regulating apoptosis-related proteins of mitochondria. International Journal of Molecular Medicine, 35(1), 218–226. [CrossRef]
19. Okamoto, K., Sugie, S., Ohnishi, M., Makita, H., Kawamori, T., Watanabe, T., Tanaka, T., Mori, H. (1996). Chemopreventive effects of taurine on diethylnitrosamine and phenobarbital-induced hepatocarcinogenesis in male F344 rats. Japanese Journal of Cancer Research, 87(1), 30–36. [CrossRef]
20. Wang, H.R. (2008). PhD Thesis. Experimental study of the effect of taurine on sarcoma 180(S180) in mices. Qingdao University, Qingdao, China.
21. Neary, P.M., Hallihan, P., Wang, J.H., Pfirrmann, R.W., Bouchier-Hayes, D.J., Redmond, H.P. (2010). The evolving role of taurolidine in cancer therapy. Annals of Surgical Oncology, 17(4), 1135–1143. [CrossRef]
22. Popescu, G.D.A., Scheau, C., Badarau, I.A., Dumitrache, M.D., Caruntu, A., Scheau, A.E., Costache, D.O., Costache, R.S., Constantin, C, Neagu, M., Caruntu, C. (2020). The effects of capsaicin on gastrointestinal cancers. Molecules, 26(1), 94. [CrossRef]
23. Zhang, R., Humphreys, I., Sahu, R.P., Shi, Y., Srivastava, S.K. (2008). In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis, 13(12), 1465-1478. [CrossRef]
24. Popescu, G., Scheau, C., Badarau, I. A., Dumitrache, M. D., Caruntu, A., Scheau, A. E., Costache, D. O., Costache, R. S., Constantin, C., Neagu, M., Caruntu, C. (2020). The Effects of Capsaicin on Gastrointestinal Cancers. Molecules (Basel, Switzerland), 26(1), 94. [CrossRef]
25. Cory, H., Passarelli, S., Szeto, J., Tamez, M., Mattei, J. (2018). The role of polyphenols in human health and food systems: A Mini-Review. Frontiers in Nutrition, 5, 87. [CrossRef]
26. Surh, Y.J., Lee, S.S. (1995). Capsaicin, a double-edged sword: toxicity, metabolism, and chemopreventive potential. Life Sciences, 56(22), 1845–1855. [CrossRef]
27. Fattori, V., Hohmann, M.S., Rossaneis, A.C., Pinho-Ribeiro, F.A., Verri, W.A. (2016). Capsaicin: Current understanding of its mechanisms and therapy of pain and other pre-clinical and clinical uses. Molecules (Basel, Switzerland), 21(7), 844. [CrossRef]
28. Bouayed, J., Bohn, T. (2010). Exogenous antioxidants--Double-edged swords in cellular redox state: Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxidative Medicine and Cellular Longevity, 3(4), 228–237. [CrossRef]
29. Cooper, D.A., Eldridge, A.L., Peters, J.C. (1999). Dietary carotenoids and certain cancers, heart disease, and age-related macular degeneration: a review of recent research. Nutrition Reviews, 57(7), 201–214. [CrossRef]
30. Hirsch, K., Atzmon, A., Danilenko, M., Levy, J., Sharoni, Y. (2007). Lycopene and other carotenoids inhibit estrogenic activity of 17beta-estradiol and genistein in cancer cells. Breast Cancer Research and Treatment, 104(2), 221–230. [CrossRef]
31. Larsson, S.C., Bergkvist, L., Wolk, A. (2010). Dietary carotenoids and risk of hormone receptor-defined breast cancer in a prospective cohort of Swedish women. European Journal of Cancer, 46(6), 1079–1085. [CrossRef]
32. Zhang, X., Spiegelman, D., Baglietto, L., Bernstein, L., Boggs, D.A., van den Brandt, P.A., Buring, J.E., Gapstur, S.M., Giles, G.G., Giovannucci, E., Goodman, G., ... Smith-Warner, S.A. (2012). Carotenoid intakes and risk of breast cancer defined by estrogen receptor and progesterone receptor status: a pooled analysis of 18 prospective cohort studies. The American Journal of Clinical Nutrition, 95(3), 713–725. [CrossRef]
33. Tordjman, S., Chokron, S., Delorme, R., Charrier, A., Bellissant, E., Jaafari, N., Fougerou, C. (2017). Melatonin: Pharmacology, Functions and Therapeutic Benefits. Current Neuropharmacology, 15(3), 434-443. [CrossRef]
34. Menéndez-Menéndez, J., Martínez-Campa, C. (2018). Melatonin: An anti-tumor agent in hormone-dependent cancers. International Journal of Endocrinology, 3271948. [CrossRef]
35. Nowfar, S., Teplitzky, S.R., Melancon, K., Kiefer, T.L., Cheng, Q., Dwived, P.D., Bischoff, E.D., Moro, K., Anderson, M.B., Dai, J., Lai, L., Yuan, L., Hill, S.M. (2002). Tumor prevention by 9-cis-retinoic acid in the N-nitroso-N-methylurea model of mammary carcinogenesis is potentiated by the pineal hormone melatonin. Breast Cancer Research and Treatment, 72(1), 33-43. [CrossRef]
36. Najafi, M., Salehi, E., Farhood, B., Nashtaei, M.S., Hashemi Goradel, N., Khanlarkhani, N., Namjoo, Z., Mortezaee, K. (2019). Adjuvant chemotherapy with melatonin for targeting human cancers: A review. Journal of Cellular Physiology, 234(3), 2356–2372. [CrossRef]

There are 36 citations in total.

Details

Primary Language	Turkish
Subjects	Pharmacology and Pharmaceutical Sciences
Journal Section	Research Article
Authors	Hande Yüce 0000-0003-2907-2019 Neşe Başak Türkmen 0000-0001-5566-8321 Dilan Aşkın Özek 0000-0001-9075-4807 Songül Ünüvar 0000-0001-8454-490X
Submission Date	September 17, 2021
Acceptance Date	October 27, 2021
Publication Date	January 29, 2022
DOI	https://doi.org/10.33483/jfpau.995491
IZ	https://izlik.org/JA23EY33TS
Published in Issue	Year 2022 Volume: 46 Issue: 1

Cite

APA	Yüce, H., Başak Türkmen, N., Aşkın Özek, D., & Ünüvar, S. (2022). TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI. Journal of Faculty of Pharmacy of Ankara University, 46(1), 48-61. https://doi.org/10.33483/jfpau.995491
AMA	1.Yüce H, Başak Türkmen N, Aşkın Özek D, Ünüvar S. TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI. J. Fac. Pharm. Ankara. 2022;46(1):48-61. doi:10.33483/jfpau.995491
Chicago	Yüce, Hande, Neşe Başak Türkmen, Dilan Aşkın Özek, and Songül Ünüvar. 2022. “TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI”. Journal of Faculty of Pharmacy of Ankara University 46 (1): 48-61. https://doi.org/10.33483/jfpau.995491.
EndNote	Yüce H, Başak Türkmen N, Aşkın Özek D, Ünüvar S (January 1, 2022) TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI. Journal of Faculty of Pharmacy of Ankara University 46 1 48–61.
IEEE	[1]H. Yüce, N. Başak Türkmen, D. Aşkın Özek, and S. Ünüvar, “TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI”, J. Fac. Pharm. Ankara, vol. 46, no. 1, pp. 48–61, Jan. 2022, doi: 10.33483/jfpau.995491.
ISNAD	Yüce, Hande - Başak Türkmen, Neşe - Aşkın Özek, Dilan - Ünüvar, Songül. “TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI”. Journal of Faculty of Pharmacy of Ankara University 46/1 (January 1, 2022): 48-61. https://doi.org/10.33483/jfpau.995491.
JAMA	1.Yüce H, Başak Türkmen N, Aşkın Özek D, Ünüvar S. TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI. J. Fac. Pharm. Ankara. 2022;46:48–61.
MLA	Yüce, Hande, et al. “TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI”. Journal of Faculty of Pharmacy of Ankara University, vol. 46, no. 1, Jan. 2022, pp. 48-61, doi:10.33483/jfpau.995491.
Vancouver	1.Yüce H, Başak Türkmen N, Aşkın Özek D, Ünüvar S. TAURİN, KAPSAİSİN, MELATONİN VE BETA KAROTENİN L929 SAĞLIKLI HÜCRELER VE MCF-7 MEME KANSERİ HÜCRELERİ ÜZERİNDEKİ ANTİPROLİFERATİF, ANTİMİGRASYON VE ANTİOKSİDAN ETKİLERİNİN ARAŞTIRILMASI. J. Fac. Pharm. Ankara [Internet]. 2022 Jan. 1;46(1):48-61. Available from: https://izlik.org/JA23EY33TS

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The Journal of Faculty of Pharmacy of Ankara University is an international medium, an open access, peer-reviewed journal for the publication of original research reports, reviews and short communications in English or Turkish on relevant developments in pharmaceutical sciences., Proceeding of scientific meetings may be published as special issues of supplements to the journal. Additionally, it contains previous and forthcoming national and international scientific meetings with social activities in pharmaceutical areas.