The Journal of Faculty of Pharmacy of Ankara University is an international medium, an open access, peer-reviewed journal for the publication of original research reports, reviews and short communications in English or Turkish on relevant developments in pharmaceutical sciences., Proceeding of scientific meetings may be published as special issues of supplements to the journal. Additionally, it contains previous and forthcoming national and international scientific meetings with social activities in pharmaceutical areas.
Research Article
FATTY ACID, TOCOPHEROL, MINERAL COMPOSITION, TOTAL PHENOLIC, FLAVONOID, THYMOQUINONE CONTENT, AND ANTIOXIDANT ACTIVITY OF NIGELLA SATIVA L.
Abstract
The fatty acid and tocopherol composition of fixed oil from seeds of Nigella sativa L. has been investigated by GC-MS and HPLC, respectively. Linoleic and oleic acids were the main fatty acids. The major tocopherols were γ- and δ-tocopherols. The total phenolic and flavonoid content and primary antioxidant activity of aqueous methanolic extracts of different parts of N.sativa were measured. Total phenolic content was assessed by Folin-Ciocalteu method and the content of flavonoids was measured spectrophotometrically by using the AlCl3 assay. Primary antioxidant activity in terms of free radical scavenging activity of the extracts was evaluated by DPPH assay. The present study showed that the seed extract contained significantly higher amount of total phenolic compounds than aerial part extract. The total flavonoid content ranged between 106.10±0.10 and 118.06±0.02 mg rutin equivalent per g extracts. The thymoquinone content in seeds were quantified by RP-HPLC analysis and the concentration of thymoquinone was 268.3 µg/mL. In DPPH radical scavenging assay, inhibition % of the extracts was found to be 95.0990.71. The seeds also were analyzed by ICP-MS to determine mineral content. P, K, Ca and Mg were predominant elements among seventeen minerals in the seeds
NIGELLA SATIVA L.’NIN YAĞ ASİDİ, TOKOFEROL, MİNERAL BİLEŞİMİ, TOTAL FENOLİK, FLAVONOİT, TİMOKİNON MİKTARI VE ANTİOXİDAN AKTİVİTESİ
Abstract
Nigella sativa L. tohumlarından elde edilen sabit yağın yağ asidi ve tokoferol bileşimi sırasıyla GCMS ve HPLC analizleri ile saptandı. Linoleik ve oleik asitler başlıca yağ asitleridir. Major tokoferoller γand δ-tokoferollerdir. Bitkinin farklı kısmlarının sulu metanollü ekstrelerinin total fenol ve flavonoit bileşimleri ve primer antioksidan aktivitesi saptandı. Total fenol bileşimi Folin-Ciocalteu metodu ve total flavonoit bileşimi AlCl3 deneyi ilespektrofotometrik yöntemle tayin edildi. Primer antioksidan aktivite açısından ekstreler DPPH yöntemi ile değerlendirildi. Yapılan çalışma tohum ekstresinin total fenol bileşiminin topraküstü ekstresinden daha yüksek miktarda olduğunu gösterdi. Total flavonoit miktarı 106.10±0.10-118.06±0.02 mg (rutin equivan/g) arasında saptandı. Tohum timokinon miktarı RP-HPLC ile belirlendi ve konsantrasyonu 268.3 µg/mg olarak saptandı. DPPH radikal süpürücü testte ekstrelerin inhibisyonunun % 95.09-90.71 arasında olduğu belirlendi. Tohumların mineral bileşimi ise ICP-MS ile saptandı. Tohumlarda saptanan onyedi mineral arasında P, K, Ca ve Mg başlıca elementler olarak belirlendi
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Details
| Primary Language | English |
|---|---|
| Subjects | Pharmacology and Pharmaceutical Sciences |
| Other ID | JA75TT47EG |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2010 |
| Submission Date | September 1, 2010 |
| Published in Issue | Year 2010 Volume: 39 Issue: 3 |