GALIUM APARINE’NİN ANTİOKSİDAN, ANTİMİKROBİYAL VE ANTİPROLİFERATİF AKTİVİTELERİ

Year 2021, Volume: 45 Issue: 3, 554 - 564, 27.09.2021

Nuh Korkmaz , Alpaslan Dayangaç , Mustafa Sevindik

https://doi.org/10.33483/jfpau.977776

Cited By: 14

Abstract

Amaç: Bitkilerin birçok biyolojik aktiviteye sahip olduğu bilinmektedir. Bu çalışmada Galium aparine L.'nin antioksidan, oksidan, antimikrobiyal ve antiproliferatif aktiviteleri araştırıldı.

Gereç ve Yöntem: Toprak üstü kısımları Soxhlet cihazında etanol ile ekstrakte edilen bitkinin antioksidan ve oksidan potansiyelleri Rel test kitleri kullanılarak ölçüldü. Bakteri ve mantarlara karşı antimikrobiyal aktivite, agar seyreltme yöntemi kullanılarak belirlendi. Antiproliferatif aktiviteyi belirlemek için Lung Carcinoma Cell Line (A549) kullanıldı.

Sonuç ve Tartışma: Çalışmalar sonucunda bitki ekstraktlarının toplam antioksidan seviyesi (TAS) değeri 5.147 ± 0.237, toplam oksidan seviyesi (TOS) değeri 18.679 ± 0.245 ve oksidatif stres indeksi (OSI) değeri 0.346 ± 0.018 olarak belirlenmiştir. Bitki ekstraktlarının 50-200 µg/mL konsantrasyonlarında test mikroorganizmalarına karşı etkili olduğu bulundu. Ayrıca bitki ekstraktının antiproliferatif aktivitesinin ekstrakt konsantrasyonundaki artışa bağlı olarak güçlü etkiler gösterdiği belirlendi. Sonuç olarak Galium aparinin yüksek biyolojik aktiviteye sahip olduğu ve bu bağlamda doğal bir farmakolojik ajan olarak kullanılabileceği belirlendi.

Keywords

Galium aparine, tıbbi bitkiler, antioksidan, antiproliferatif

Supporting Institution

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Project Number

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Thanks

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ANTIOXIDANT, ANTIMICROBIAL AND ANTIPROLIFERATIVE ACTIVITIES OF GALIUM APARINE

Abstract

Objective: Plants are known to have many biological activities. In this study, the antioxidant, oxidant, antimicrobial and antiproliferative activities of Galium aparine L. were investigated.

Material and Method: The antioxidant and oxidant potentials of the plant, the aerial parts of which were extracted with ethanol in a Soxhlet device, were measured using Rel assay kits. Antimicrobial activity against bacteria and fungi was determined using the agar dilution method. Lung carcinoma cell line (A549) was used to determine the antiproliferative activity.

Result and Discussion: As a result of the studies, total antioxidant status (TAS) value of plant extracts was determined as 5.147 ± 0.237, total oxidant status (TOS) value as 18.679 ± 0.245 and oxidative stress index (OSI) value as 0.346 ± 0.018. Plant extracts were found to be effective against test microorganisms at concentrations of 50-200 µg/mL. In addition, it was determined that the antiproliferative activity of the plant extract showed potent effects depending on the increase in the extract concentration. Finally, it was determined that Galium aparine has high biological activity and can be used as a natural pharmacological agent in this context.

Keywords

Galium aparine, medicinal plants, antioxidant, antiproliferative

Project Number

Yok

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